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High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis

High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis
High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis
Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.
1359-7345
1506-1509
Jiang, Haibo
332f707e-1f2f-41ec-9843-dca733a1700a
Passarelli, Melissa K.
655899fd-514a-44c5-bb7b-9cb4302f9f2f
Munro, Peter M.G.
191ac286-e9cb-486d-ab96-d0e684435353
Kilburn, Matt R.
b3ace173-13a1-47cd-9365-344a0fb9566d
West, Andrew
fa95ae31-5789-4368-af47-55f4ca8c281a
Dollery, Colin T.
e8e1cee1-e678-426e-a0a8-064ebc9160bf
Gilmore, Ian S.
f864335b-9cb0-4f44-b80f-30809227212b
Rakowska, Paulina D.
73bf2145-e2fa-46ee-9ad9-f74c80d5a369
Jiang, Haibo
332f707e-1f2f-41ec-9843-dca733a1700a
Passarelli, Melissa K.
655899fd-514a-44c5-bb7b-9cb4302f9f2f
Munro, Peter M.G.
191ac286-e9cb-486d-ab96-d0e684435353
Kilburn, Matt R.
b3ace173-13a1-47cd-9365-344a0fb9566d
West, Andrew
fa95ae31-5789-4368-af47-55f4ca8c281a
Dollery, Colin T.
e8e1cee1-e678-426e-a0a8-064ebc9160bf
Gilmore, Ian S.
f864335b-9cb0-4f44-b80f-30809227212b
Rakowska, Paulina D.
73bf2145-e2fa-46ee-9ad9-f74c80d5a369

Jiang, Haibo, Passarelli, Melissa K., Munro, Peter M.G., Kilburn, Matt R., West, Andrew, Dollery, Colin T., Gilmore, Ian S. and Rakowska, Paulina D. (2017) High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis. ChemComm, 53 (9), 1506-1509. (doi:10.1039/C6CC08549K).

Record type: Article

Abstract

Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.

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More information

Accepted/In Press date: 7 December 2016
e-pub ahead of print date: 7 December 2016
Published date: 31 January 2017

Identifiers

Local EPrints ID: 445059
URI: http://eprints.soton.ac.uk/id/eprint/445059
ISSN: 1359-7345
PURE UUID: 8ad565d8-e87d-402a-a3e3-a85f5b17fbbb
ORCID for Paulina D. Rakowska: ORCID iD orcid.org/0000-0002-3710-8395

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Date deposited: 18 Nov 2020 17:33
Last modified: 28 Apr 2022 02:31

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Contributors

Author: Haibo Jiang
Author: Melissa K. Passarelli
Author: Peter M.G. Munro
Author: Matt R. Kilburn
Author: Andrew West
Author: Colin T. Dollery
Author: Ian S. Gilmore

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