MiS-MALDI: microgel-selected detection of protein biomarkers by MALDI-ToF mass spectrometry

Cerasoli, Eleonora, Rakowska, Paulina D., Horgan, Adrian, Ravi, Jascindra, Bradley, Melanie, Vincent, Brian and Ryadnov, Maxim G. (2010) MiS-MALDI: microgel-selected detection of protein biomarkers by MALDI-ToF mass spectrometry. Molecular BioSystems, 6 (11), 2214-2217. (doi:10.1039/c0mb00073f).

Abstract

Intensified efforts to decipher the origin of disease at the molecular level stimulate the emergence of more efficient proteomic technologies. To complement this, attempts are being made to identify new predictive biomarkers for building more reliable biomarker patterns. As biomarker research gathers pace an immediate interest becomes focused on platforms, which although based on mainstream approaches, are more amenable to specialist tasks. Particularly relevant this is for disease-specific biomarkers, which are present at very low concentrations in multicomponent biological fluids and require depletion protocols enabling their separation from high-abundance components. In this report, we describe a new strategy allowing the rapid detection of target protein biomarkers by MALDI-ToF mass spectrometry. The approach relies on selective sequestering of target proteins from complex media by engineered microgels, which select proteins by their size (

This record has no associated files available for download.

Contributors

Author: Paulina D. Rakowska

Download statistics