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MiS-MALDI: microgel-selected detection of protein biomarkers by MALDI-ToF mass spectrometry

MiS-MALDI: microgel-selected detection of protein biomarkers by MALDI-ToF mass spectrometry
MiS-MALDI: microgel-selected detection of protein biomarkers by MALDI-ToF mass spectrometry
Intensified efforts to decipher the origin of disease at the molecular level stimulate the emergence of more efficient proteomic technologies. To complement this, attempts are being made to identify new predictive biomarkers for building more reliable biomarker patterns. As biomarker research gathers pace an immediate interest becomes focused on platforms, which although based on mainstream approaches, are more amenable to specialist tasks. Particularly relevant this is for disease-specific biomarkers, which are present at very low concentrations in multicomponent biological fluids and require depletion protocols enabling their separation from high-abundance components. In this report, we describe a new strategy allowing the rapid detection of target protein biomarkers by MALDI-ToF mass spectrometry. The approach relies on selective sequestering of target proteins from complex media by engineered microgels, which select proteins by their size (
1742-206X
2214-2217
Cerasoli, Eleonora
18ce2ec4-cab6-4367-8c8c-b6ba12d8ab55
Rakowska, Paulina D.
73bf2145-e2fa-46ee-9ad9-f74c80d5a369
Horgan, Adrian
e8858b15-7e32-44be-a437-966fa317d2e4
Ravi, Jascindra
d0c93c4d-3a20-4b47-83de-588adc502509
Bradley, Melanie
edef9596-9f04-4741-a7cb-47dc54c466fd
Vincent, Brian
b5d62d07-5939-448c-9ab7-11b6535342b9
Ryadnov, Maxim G.
5bd99202-02e9-42e6-b56f-300852222d06
Cerasoli, Eleonora
18ce2ec4-cab6-4367-8c8c-b6ba12d8ab55
Rakowska, Paulina D.
73bf2145-e2fa-46ee-9ad9-f74c80d5a369
Horgan, Adrian
e8858b15-7e32-44be-a437-966fa317d2e4
Ravi, Jascindra
d0c93c4d-3a20-4b47-83de-588adc502509
Bradley, Melanie
edef9596-9f04-4741-a7cb-47dc54c466fd
Vincent, Brian
b5d62d07-5939-448c-9ab7-11b6535342b9
Ryadnov, Maxim G.
5bd99202-02e9-42e6-b56f-300852222d06

Cerasoli, Eleonora, Rakowska, Paulina D., Horgan, Adrian, Ravi, Jascindra, Bradley, Melanie, Vincent, Brian and Ryadnov, Maxim G. (2010) MiS-MALDI: microgel-selected detection of protein biomarkers by MALDI-ToF mass spectrometry. Molecular BioSystems, 6 (11), 2214-2217. (doi:10.1039/c0mb00073f).

Record type: Article

Abstract

Intensified efforts to decipher the origin of disease at the molecular level stimulate the emergence of more efficient proteomic technologies. To complement this, attempts are being made to identify new predictive biomarkers for building more reliable biomarker patterns. As biomarker research gathers pace an immediate interest becomes focused on platforms, which although based on mainstream approaches, are more amenable to specialist tasks. Particularly relevant this is for disease-specific biomarkers, which are present at very low concentrations in multicomponent biological fluids and require depletion protocols enabling their separation from high-abundance components. In this report, we describe a new strategy allowing the rapid detection of target protein biomarkers by MALDI-ToF mass spectrometry. The approach relies on selective sequestering of target proteins from complex media by engineered microgels, which select proteins by their size (

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Published date: 23 August 2010

Identifiers

Local EPrints ID: 445181
URI: http://eprints.soton.ac.uk/id/eprint/445181
ISSN: 1742-206X
PURE UUID: 9ec8ad0b-23e7-4f78-9454-447792243e22

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Date deposited: 24 Nov 2020 17:34
Last modified: 24 Nov 2020 17:34

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Contributors

Author: Eleonora Cerasoli
Author: Adrian Horgan
Author: Jascindra Ravi
Author: Melanie Bradley
Author: Brian Vincent
Author: Maxim G. Ryadnov

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