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Stable isotope imaging of biological samples with high resolution secondary ion mass spectrometry and complementary techniques

Stable isotope imaging of biological samples with high resolution secondary ion mass spectrometry and complementary techniques
Stable isotope imaging of biological samples with high resolution secondary ion mass spectrometry and complementary techniques
Stable isotopes are ideal labels for studying biological processes because they have little or no effect on the biochemical properties of target molecules. The NanoSIMS is a tool that can image the distribution of stable isotope labels with up to 50 nm spatial resolution and with good quantitation. This combination of features has enabled several groups to undertake significant experiments on biological problems in the last decade. Combining the NanoSIMS with other imaging techniques also enables us to obtain not only chemical information but also the structural information needed to understand biological processes. This article describes the methodologies that we have developed to correlate atomic force microscopy and backscattered electron imaging with NanoSIMS experiments to illustrate the imaging of stable isotopes at molecular, cellular, and tissue scales. Our studies make it possible to address 3 biological problems: (1) the interaction of antimicrobial peptides with membranes; (2) glutamine metabolism in cancer cells; and (3) lipoprotein interactions in different tissues.
Stable isotope NanoSIMS Atomic force microscopy Backscattered electron imaging Correlative analysis
1046-2023
317-324
Jiang, H.
6930de85-44dd-42ad-9dc8-21532c894510
Favaro, E.
c4294b7a-f85d-462c-bc0a-b5982135d22d
Goulbourne, C. N.
52272cd3-4bf0-4a0f-b037-6eff8964df61
Rakowska, P. D.
73bf2145-e2fa-46ee-9ad9-f74c80d5a369
Hughes, G. M.
bce7b43f-125a-410a-a3ed-1924ab92a31f
Ryadnov, M. G.
5bd99202-02e9-42e6-b56f-300852222d06
Fong, L. G.
570ae14d-7ea9-4d63-80d3-89afa4280f4d
Ferguson, D. J. P.
55255b70-9dd0-46af-834c-b68b35324da2
Grovenor, C. R. M.
e6802cf5-a041-47e9-80cf-e4fd65562494
Jiang, H.
6930de85-44dd-42ad-9dc8-21532c894510
Favaro, E.
c4294b7a-f85d-462c-bc0a-b5982135d22d
Goulbourne, C. N.
52272cd3-4bf0-4a0f-b037-6eff8964df61
Rakowska, P. D.
73bf2145-e2fa-46ee-9ad9-f74c80d5a369
Hughes, G. M.
bce7b43f-125a-410a-a3ed-1924ab92a31f
Ryadnov, M. G.
5bd99202-02e9-42e6-b56f-300852222d06
Fong, L. G.
570ae14d-7ea9-4d63-80d3-89afa4280f4d
Ferguson, D. J. P.
55255b70-9dd0-46af-834c-b68b35324da2
Grovenor, C. R. M.
e6802cf5-a041-47e9-80cf-e4fd65562494

Jiang, H., Favaro, E., Goulbourne, C. N., Rakowska, P. D., Hughes, G. M., Ryadnov, M. G., Fong, L. G., Ferguson, D. J. P. and Grovenor, C. R. M. (2014) Stable isotope imaging of biological samples with high resolution secondary ion mass spectrometry and complementary techniques. Methods, 68 (2), 317-324. (doi:10.1016/j.ymeth.2014.02.012).

Record type: Article

Abstract

Stable isotopes are ideal labels for studying biological processes because they have little or no effect on the biochemical properties of target molecules. The NanoSIMS is a tool that can image the distribution of stable isotope labels with up to 50 nm spatial resolution and with good quantitation. This combination of features has enabled several groups to undertake significant experiments on biological problems in the last decade. Combining the NanoSIMS with other imaging techniques also enables us to obtain not only chemical information but also the structural information needed to understand biological processes. This article describes the methodologies that we have developed to correlate atomic force microscopy and backscattered electron imaging with NanoSIMS experiments to illustrate the imaging of stable isotopes at molecular, cellular, and tissue scales. Our studies make it possible to address 3 biological problems: (1) the interaction of antimicrobial peptides with membranes; (2) glutamine metabolism in cancer cells; and (3) lipoprotein interactions in different tissues.

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More information

Published date: 17 February 2014
Keywords: Stable isotope NanoSIMS Atomic force microscopy Backscattered electron imaging Correlative analysis

Identifiers

Local EPrints ID: 445182
URI: http://eprints.soton.ac.uk/id/eprint/445182
ISSN: 1046-2023
PURE UUID: e8b8dc1c-5f96-4e03-9451-3f84279e9efe
ORCID for P. D. Rakowska: ORCID iD orcid.org/0000-0002-3710-8395

Catalogue record

Date deposited: 24 Nov 2020 17:34
Last modified: 28 Apr 2022 02:31

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Contributors

Author: H. Jiang
Author: E. Favaro
Author: C. N. Goulbourne
Author: P. D. Rakowska ORCID iD
Author: G. M. Hughes
Author: M. G. Ryadnov
Author: L. G. Fong
Author: D. J. P. Ferguson
Author: C. R. M. Grovenor

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