The University of Southampton
University of Southampton Institutional Repository

Evidence of homologous recombination as a driver of diversity in Brachyspira pilosicoli

Evidence of homologous recombination as a driver of diversity in Brachyspira pilosicoli
Evidence of homologous recombination as a driver of diversity in Brachyspira pilosicoli
The enteric, pathogenic spirochaete Brachyspira pilosicoli colonizes and infects a variety of birds and mammals, including humans. However, there is a paucity of genomic data available for this organism. This study introduces 12 newly sequenced draft genome assemblies, boosting the cohort of examined isolates by fourfold and cataloguing the intraspecific genomic diversity of the organism more comprehensively. We used several in silico techniques to define a core genome of 1751 genes and qualitatively and quantitatively examined the intraspecific species boundary using phylogenetic analysis and average nucleotide identity, before contextualizing this diversity against other members of the genus Brachyspira. Our study revealed that an additional isolate that was unable to be species typed against any other Brachyspira lacked putative virulence factors present in all other isolates. Finally, we quantified that homologous recombination has as great an effect on the evolution of the core genome of the B. pilosicoli as random mutation (r/m=1.02). Comparative genomics has informed Brachyspira diversity, population structure, host specificity and virulence. The data presented here can be used to contribute to developing advanced screening methods, diagnostic assays and prophylactic vaccines against this zoonotic pathogen.
BACTERIA, bacterial evolution, microevolution, zoonosis, Homologous recombination, genomics
2057-5858
1-15
Pandey, Anish
bfb6596d-f222-481c-a594-329a8cc87043
Humbert, Maria
82134d25-24b8-4fdd-bd1c-461683b5322e
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Jackson, Alexandra Charlotte
eb2e2dd5-0d2c-47af-89a9-09b82f4b4ccb
Passey, Jade
02a8c8ee-b64d-44ab-aadd-b5b39d2176ad
La Ragione, Robert
897fa251-b21e-4c63-85f3-482b64980eca
Hampson, David
b395030e-de65-47f6-8d68-f5c569b0c4e6
Cleary, David
f4079c6d-d54b-4108-b346-b0069035bec0
Pandey, Anish
bfb6596d-f222-481c-a594-329a8cc87043
Humbert, Maria
82134d25-24b8-4fdd-bd1c-461683b5322e
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Jackson, Alexandra Charlotte
eb2e2dd5-0d2c-47af-89a9-09b82f4b4ccb
Passey, Jade
02a8c8ee-b64d-44ab-aadd-b5b39d2176ad
La Ragione, Robert
897fa251-b21e-4c63-85f3-482b64980eca
Hampson, David
b395030e-de65-47f6-8d68-f5c569b0c4e6
Cleary, David
f4079c6d-d54b-4108-b346-b0069035bec0

Pandey, Anish, Humbert, Maria, Christodoulides, Myron, Jackson, Alexandra Charlotte, Passey, Jade, La Ragione, Robert, Hampson, David and Cleary, David (2020) Evidence of homologous recombination as a driver of diversity in Brachyspira pilosicoli. Microbial Genomics, 1-15. (doi:10.1099/mgen.0.000470).

Record type: Article

Abstract

The enteric, pathogenic spirochaete Brachyspira pilosicoli colonizes and infects a variety of birds and mammals, including humans. However, there is a paucity of genomic data available for this organism. This study introduces 12 newly sequenced draft genome assemblies, boosting the cohort of examined isolates by fourfold and cataloguing the intraspecific genomic diversity of the organism more comprehensively. We used several in silico techniques to define a core genome of 1751 genes and qualitatively and quantitatively examined the intraspecific species boundary using phylogenetic analysis and average nucleotide identity, before contextualizing this diversity against other members of the genus Brachyspira. Our study revealed that an additional isolate that was unable to be species typed against any other Brachyspira lacked putative virulence factors present in all other isolates. Finally, we quantified that homologous recombination has as great an effect on the evolution of the core genome of the B. pilosicoli as random mutation (r/m=1.02). Comparative genomics has informed Brachyspira diversity, population structure, host specificity and virulence. The data presented here can be used to contribute to developing advanced screening methods, diagnostic assays and prophylactic vaccines against this zoonotic pathogen.

Text
PANDEYmgen000470 - Version of Record
Available under License Creative Commons Attribution Share Alike.
Download (3MB)
Text
PANDEYmgen000470
Restricted to Repository staff only
Request a copy

More information

Accepted/In Press date: 16 October 2020
e-pub ahead of print date: 11 November 2020
Published date: 11 November 2020
Keywords: BACTERIA, bacterial evolution, microevolution, zoonosis, Homologous recombination, genomics

Identifiers

Local EPrints ID: 445219
URI: http://eprints.soton.ac.uk/id/eprint/445219
ISSN: 2057-5858
PURE UUID: aa76a89f-1a1e-421b-844a-ae96b77f82dd
ORCID for Maria Humbert: ORCID iD orcid.org/0000-0002-5728-6981
ORCID for Myron Christodoulides: ORCID iD orcid.org/0000-0002-9663-4731
ORCID for David Cleary: ORCID iD orcid.org/0000-0003-4533-0700

Catalogue record

Date deposited: 25 Nov 2020 17:32
Last modified: 07 Oct 2021 01:46

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×