DNA triple-helix formation at target sites containing duplex mismatches
DNA triple-helix formation at target sites containing duplex mismatches
We have studied the formation of DNA triple helices at target sites that contain mismatches in the duplex target. Fluorescence melting studies were used to examine a series of parallel triple helices that contain all 64 N.XZ triplet combinations at the centre (where N, X and Z are each of the four natural DNA bases in turn). Similar experiments were also performed with N = bis-amino-U (BAU) (for stable recognition of AT base pairs) and N = S (for recognition of TA inversions). We find that the introduction of a duplex mismatch destabilises the C+.GZ, T.AZ and G.TZ triplets. A similar effect is seen with BAU.AZ triplets. In contrast, other base combinations, based on non-standard triplets such as C.AZ, T.TZ, G.CZ and A. CZ are stabilised by the presence of a duplex mismatch. In each case S binds to sites containing duplex mismatches better than the corresponding Watson-Crick base pairs.
triple helix, mismatch, thermal melting forming oligonucleotides, base-pairs, physiological ph, recognition, motif, nucleoside, guanine, ta, cg
134-140
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
September 2006
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Rusling, David A., Brown, Tom and Fox, Keith R.
(2006)
DNA triple-helix formation at target sites containing duplex mismatches.
Biophysical Chemistry, 123 (2-3), .
(doi:10.1016/j.bpc.2006.04.016).
Abstract
We have studied the formation of DNA triple helices at target sites that contain mismatches in the duplex target. Fluorescence melting studies were used to examine a series of parallel triple helices that contain all 64 N.XZ triplet combinations at the centre (where N, X and Z are each of the four natural DNA bases in turn). Similar experiments were also performed with N = bis-amino-U (BAU) (for stable recognition of AT base pairs) and N = S (for recognition of TA inversions). We find that the introduction of a duplex mismatch destabilises the C+.GZ, T.AZ and G.TZ triplets. A similar effect is seen with BAU.AZ triplets. In contrast, other base combinations, based on non-standard triplets such as C.AZ, T.TZ, G.CZ and A. CZ are stabilised by the presence of a duplex mismatch. In each case S binds to sites containing duplex mismatches better than the corresponding Watson-Crick base pairs.
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Published date: September 2006
Keywords:
triple helix, mismatch, thermal melting forming oligonucleotides, base-pairs, physiological ph, recognition, motif, nucleoside, guanine, ta, cg
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Local EPrints ID: 44536
URI: http://eprints.soton.ac.uk/id/eprint/44536
ISSN: 0301-4622
PURE UUID: eb2310f0-886b-4fb9-82bd-0a0349d917ea
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Date deposited: 07 Mar 2007
Last modified: 16 Mar 2024 02:36
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Author:
David A. Rusling
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