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DNA triple-helix formation at target sites containing duplex mismatches

Record type: Article

We have studied the formation of DNA triple helices at target sites that contain mismatches in the duplex target. Fluorescence melting studies were used to examine a series of parallel triple helices that contain all 64 N.XZ triplet combinations at the centre (where N, X and Z are each of the four natural DNA bases in turn). Similar experiments were also performed with N = bis-amino-U (BAU) (for stable recognition of AT base pairs) and N = S (for recognition of TA inversions). We find that the introduction of a duplex mismatch destabilises the C+.GZ, T.AZ and G.TZ triplets. A similar effect is seen with BAU.AZ triplets. In contrast, other base combinations, based on non-standard triplets such as C.AZ, T.TZ, G.CZ and A. CZ are stabilised by the presence of a duplex mismatch. In each case S binds to sites containing duplex mismatches better than the corresponding Watson-Crick base pairs.

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Citation

Rusling, David A., Brown, Tom and Fox, Keith R. (2006) DNA triple-helix formation at target sites containing duplex mismatches Biophysical Chemistry, 123, (2-3), pp. 134-140. (doi:10.1016/j.bpc.2006.04.016).

More information

Published date: September 2006
Keywords: triple helix, mismatch, thermal melting forming oligonucleotides, base-pairs, physiological ph, recognition, motif, nucleoside, guanine, ta, cg

Identifiers

Local EPrints ID: 44536
URI: http://eprints.soton.ac.uk/id/eprint/44536
ISSN: 0301-4622
PURE UUID: eb2310f0-886b-4fb9-82bd-0a0349d917ea
ORCID for David A. Rusling: ORCID iD orcid.org/0000-0002-7442-686X
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 07 Mar 2007
Last modified: 17 Jul 2017 15:14

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