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Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG and two-photon microscopy

Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG and two-photon microscopy
Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG and two-photon microscopy
We demonstrate a continuous wave (CW) seeded synchronization-free optical parametric amplifier (OPA) pumped by a picosecond, 1 μm laser and show its performance when used as a simple yet powerful source for label-free Coherent anti-Stokes Raman scattering (CARS) and concurrent Second Harmonic Generation (SHG) and two-photon fluorescence microscopy in an epi-detection geometry. The average power level of above 175 mW, spectral resolution of 8 cm−1, and 2 ps pulse duration are well optimized for CARS microscopy in bio-science and bio-medical imaging systems. Our OPA is a much simpler setup than either the ‘gold-standard’ laser and optical parametric oscillator (OPO) combination traditionally used for CARS imaging, or the more recently developed OPA systems pumped with femtosecond pulses [1]. Rapid and accurate tuning between resonances was achieved by changing the poled channels and temperature of the periodically-poled lithium niobate (PPLN) OPA crystal together with the OPA seed wavelength. The Pump-Stokes frequency detuning range fully covered the C-H stretching band used for the imaging of lipids. By enabling three multiphoton techniques using a compact, synchronization free laser source, our work paves the way for the translation of label-free multi-photon microscopy imaging from biomedical research to an imaging based diagnostic tool for use in the healthcare arena.
2156-7085
Xu, Duanyang
2eeb11e3-d31e-4552-a925-49a3ffd7da20
Price, Jonathan
fddcce17-291b-4d01-bd38-8fb0453abdc8
Xu, Lin
b887cecd-d21e-49f4-9b45-6909a7369e84
Richardson, David
ebfe1ff9-d0c2-4e52-b7ae-c1b13bccdef3
Xu, Duanyang
2eeb11e3-d31e-4552-a925-49a3ffd7da20
Price, Jonathan
fddcce17-291b-4d01-bd38-8fb0453abdc8
Xu, Lin
b887cecd-d21e-49f4-9b45-6909a7369e84
Richardson, David
ebfe1ff9-d0c2-4e52-b7ae-c1b13bccdef3

Xu, Duanyang, Price, Jonathan, Xu, Lin and Richardson, David (2020) Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG and two-photon microscopy. Biomedical Optics Express. (doi:10.1364/BOE.411620). (In Press)

Record type: Article

Abstract

We demonstrate a continuous wave (CW) seeded synchronization-free optical parametric amplifier (OPA) pumped by a picosecond, 1 μm laser and show its performance when used as a simple yet powerful source for label-free Coherent anti-Stokes Raman scattering (CARS) and concurrent Second Harmonic Generation (SHG) and two-photon fluorescence microscopy in an epi-detection geometry. The average power level of above 175 mW, spectral resolution of 8 cm−1, and 2 ps pulse duration are well optimized for CARS microscopy in bio-science and bio-medical imaging systems. Our OPA is a much simpler setup than either the ‘gold-standard’ laser and optical parametric oscillator (OPO) combination traditionally used for CARS imaging, or the more recently developed OPA systems pumped with femtosecond pulses [1]. Rapid and accurate tuning between resonances was achieved by changing the poled channels and temperature of the periodically-poled lithium niobate (PPLN) OPA crystal together with the OPA seed wavelength. The Pump-Stokes frequency detuning range fully covered the C-H stretching band used for the imaging of lipids. By enabling three multiphoton techniques using a compact, synchronization free laser source, our work paves the way for the translation of label-free multi-photon microscopy imaging from biomedical research to an imaging based diagnostic tool for use in the healthcare arena.

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More information

Accepted/In Press date: 7 December 2020

Identifiers

Local EPrints ID: 445523
URI: http://eprints.soton.ac.uk/id/eprint/445523
ISSN: 2156-7085
PURE UUID: d2e409a0-9a4e-4a46-9ca5-51006257943c
ORCID for Duanyang Xu: ORCID iD orcid.org/0000-0002-9122-1049
ORCID for Jonathan Price: ORCID iD orcid.org/0000-0003-0256-9172
ORCID for David Richardson: ORCID iD orcid.org/0000-0002-7751-1058

Catalogue record

Date deposited: 14 Dec 2020 17:32
Last modified: 13 Dec 2021 03:31

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Contributors

Author: Duanyang Xu ORCID iD
Author: Jonathan Price ORCID iD
Author: Lin Xu

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