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Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion

Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion
Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion
In modern societies, biodegradation of hydrophobic pollutants generated by industry is important for environmental and human health. In Gram-negative bacteria, biodegradation depends on facilitated diffusion of the pollutant substrates into the cell, mediated by specialised outer membrane (OM) channels. Here we show, via a combined experimental and computational approach, that the uptake of monoaromatic hydrocarbons such as toluene in Pseudomonas putida F1 (PpF1) occurs via lateral diffusion through FadL channels. Contrary to classical diffusion channels via which polar substrates move directly into the periplasmic space, PpF1 TodX and CymD direct their hydrophobic substrates into the OM via a lateral opening in the channel wall, bypassing the polar barrier formed by the lipopolysaccharide leaflet on the cell surface. Our study suggests that lateral diffusion of hydrophobic molecules is the modus operandi of all FadL channels, with potential implications for diverse areas such as biodegradation, quorum sensing and gut biology
2041-1723
Somboon, Kamolrat
78095e59-bba0-4a24-9aeb-ea1e1121da89
Doble, Anne
ac000822-c9a4-42e7-863f-22defd279ce8
Bulmer, David
3bd36013-5ecb-41ed-b700-efcc9aaa4a92
Basle, Arnaud
634a4c07-c904-46b1-99d0-5beba63f52fa
Khalid, Syma
90fbd954-7248-4f47-9525-4d6af9636394
van den Berg, Bert
d24aad29-10a4-4ffa-b893-58ba00a413f0
Somboon, Kamolrat
78095e59-bba0-4a24-9aeb-ea1e1121da89
Doble, Anne
ac000822-c9a4-42e7-863f-22defd279ce8
Bulmer, David
3bd36013-5ecb-41ed-b700-efcc9aaa4a92
Basle, Arnaud
634a4c07-c904-46b1-99d0-5beba63f52fa
Khalid, Syma
90fbd954-7248-4f47-9525-4d6af9636394
van den Berg, Bert
d24aad29-10a4-4ffa-b893-58ba00a413f0

Somboon, Kamolrat, Doble, Anne, Bulmer, David, Basle, Arnaud, Khalid, Syma and van den Berg, Bert (2020) Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion. Nature Communications, 11 (1), [6331]. (doi:10.1038/s41467-020-20126-y).

Record type: Article

Abstract

In modern societies, biodegradation of hydrophobic pollutants generated by industry is important for environmental and human health. In Gram-negative bacteria, biodegradation depends on facilitated diffusion of the pollutant substrates into the cell, mediated by specialised outer membrane (OM) channels. Here we show, via a combined experimental and computational approach, that the uptake of monoaromatic hydrocarbons such as toluene in Pseudomonas putida F1 (PpF1) occurs via lateral diffusion through FadL channels. Contrary to classical diffusion channels via which polar substrates move directly into the periplasmic space, PpF1 TodX and CymD direct their hydrophobic substrates into the OM via a lateral opening in the channel wall, bypassing the polar barrier formed by the lipopolysaccharide leaflet on the cell surface. Our study suggests that lateral diffusion of hydrophobic molecules is the modus operandi of all FadL channels, with potential implications for diverse areas such as biodegradation, quorum sensing and gut biology

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More information

Accepted/In Press date: 12 November 2020
e-pub ahead of print date: 10 December 2020

Identifiers

Local EPrints ID: 445524
URI: http://eprints.soton.ac.uk/id/eprint/445524
ISSN: 2041-1723
PURE UUID: d6a4843e-d901-4a8e-8ec1-8ea3717851c8
ORCID for Syma Khalid: ORCID iD orcid.org/0000-0002-3694-5044

Catalogue record

Date deposited: 14 Dec 2020 17:32
Last modified: 20 Apr 2021 01:42

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