DNA stable-isotope probing highlights the effects of temperature on functionally active methanotrophs in natural wetlands
DNA stable-isotope probing highlights the effects of temperature on functionally active methanotrophs in natural wetlands
Microbial methane (CH
4) oxidation is a major global sink of CH
4. Aerobic CH
4-oxidizing bacteria (methanotrophs) represent a biological model system for CH
4 consumption and is very sensitive to climate warming, but still poorly understood. Here we used DNA stable-isotope probing (SIP) coupled with high-throughput sequencing of
13C-DNA to compare active methanotrophs incubated at 10, 15, 20, and 25 °C in
13CH
4-fed microcosms from two geographically distinct natural wetlands: Sanjiang Plain wetland in northeast China and Haibei wetland in Tibet Plateau. In both wetlands, CH
4 oxidation potential was enhanced with increasing temperature. Community profiling revealed that type I methanotrophs dominated CH
4 oxidation, although a small portion (2.76%–17.14%) of type II methanotrophs (Methylocystis, Methylosinus/Methylocystis) were significantly stimulated at 20 °C and 25 °C.
13C-labeled indicator species included Methylobacter, Methylocystis, and Methylosarcina species in Sanjiang Plain, and Methylobacter and Methylosarcina species in Haibei. Network analysis demonstrated positive co-occurrence of species between genera of Methylobacter, Methylosarcina, and Methylocystis with shifts in temperature, while interspecies interactions between Methylobacter and Methylomonas correlated negatively, and Methylobacter and Methylosinus/Methylocystis positively. Partial least squares path modeling illustrated that the direct effects of temperature on CH
4 oxidation were stronger in northeast China than Tibet Plateau, and temperature could also indirectly influence CH
4 oxidation via shifts in the methanotroph communities. Collectively, these results provide insights into how temperature could influence methanotrophy in natural wetlands under future climate scenarios.
DNA-SIP, Methanotrophs, Natural wetlands, Warming, pmoA
Zhang, Liyan
507829ab-fa08-4b28-9767-44a19364e4f5
Dumont, Marc G.
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Bodelier, Paul L.e.
32eb4088-8cd2-42c4-bbe0-3589ec4db8cc
Adams, Jonathan M.
4b6ebb9c-c9bb-44db-89e5-a0adc453133a
He, Dan
34533cc2-ce86-4154-9f92-dab79b9c4404
Chu, Haiyan
d4f0f658-2b5e-4387-9604-11842a37bcce
1 October 2020
Zhang, Liyan
507829ab-fa08-4b28-9767-44a19364e4f5
Dumont, Marc G.
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Bodelier, Paul L.e.
32eb4088-8cd2-42c4-bbe0-3589ec4db8cc
Adams, Jonathan M.
4b6ebb9c-c9bb-44db-89e5-a0adc453133a
He, Dan
34533cc2-ce86-4154-9f92-dab79b9c4404
Chu, Haiyan
d4f0f658-2b5e-4387-9604-11842a37bcce
Zhang, Liyan, Dumont, Marc G., Bodelier, Paul L.e., Adams, Jonathan M., He, Dan and Chu, Haiyan
(2020)
DNA stable-isotope probing highlights the effects of temperature on functionally active methanotrophs in natural wetlands.
Soil Biology and Biochemistry, 149, [107954].
(doi:10.1016/j.soilbio.2020.107954).
Abstract
Microbial methane (CH
4) oxidation is a major global sink of CH
4. Aerobic CH
4-oxidizing bacteria (methanotrophs) represent a biological model system for CH
4 consumption and is very sensitive to climate warming, but still poorly understood. Here we used DNA stable-isotope probing (SIP) coupled with high-throughput sequencing of
13C-DNA to compare active methanotrophs incubated at 10, 15, 20, and 25 °C in
13CH
4-fed microcosms from two geographically distinct natural wetlands: Sanjiang Plain wetland in northeast China and Haibei wetland in Tibet Plateau. In both wetlands, CH
4 oxidation potential was enhanced with increasing temperature. Community profiling revealed that type I methanotrophs dominated CH
4 oxidation, although a small portion (2.76%–17.14%) of type II methanotrophs (Methylocystis, Methylosinus/Methylocystis) were significantly stimulated at 20 °C and 25 °C.
13C-labeled indicator species included Methylobacter, Methylocystis, and Methylosarcina species in Sanjiang Plain, and Methylobacter and Methylosarcina species in Haibei. Network analysis demonstrated positive co-occurrence of species between genera of Methylobacter, Methylosarcina, and Methylocystis with shifts in temperature, while interspecies interactions between Methylobacter and Methylomonas correlated negatively, and Methylobacter and Methylosinus/Methylocystis positively. Partial least squares path modeling illustrated that the direct effects of temperature on CH
4 oxidation were stronger in northeast China than Tibet Plateau, and temperature could also indirectly influence CH
4 oxidation via shifts in the methanotroph communities. Collectively, these results provide insights into how temperature could influence methanotrophy in natural wetlands under future climate scenarios.
This record has no associated files available for download.
More information
Accepted/In Press date: 6 August 2020
e-pub ahead of print date: 7 August 2020
Published date: 1 October 2020
Keywords:
DNA-SIP, Methanotrophs, Natural wetlands, Warming, pmoA
Identifiers
Local EPrints ID: 445618
URI: http://eprints.soton.ac.uk/id/eprint/445618
ISSN: 0038-0717
PURE UUID: 0e26246b-b5f0-44e5-a190-8ca7cb89b294
Catalogue record
Date deposited: 18 Dec 2020 17:31
Last modified: 17 Mar 2024 03:39
Export record
Altmetrics
Contributors
Author:
Liyan Zhang
Author:
Paul L.e. Bodelier
Author:
Jonathan M. Adams
Author:
Dan He
Author:
Haiyan Chu
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics