Zhang, Liyan, Dumont, Marc G., Bodelier, Paul L.e., Adams, Jonathan M., He, Dan and Chu, Haiyan (2020) DNA stable-isotope probing highlights the effects of temperature on functionally active methanotrophs in natural wetlands. Soil Biology and Biochemistry, 149, [107954]. (doi:10.1016/j.soilbio.2020.107954).
Abstract
Microbial methane (CH 4) oxidation is a major global sink of CH 4. Aerobic CH 4-oxidizing bacteria (methanotrophs) represent a biological model system for CH 4 consumption and is very sensitive to climate warming, but still poorly understood. Here we used DNA stable-isotope probing (SIP) coupled with high-throughput sequencing of 13C-DNA to compare active methanotrophs incubated at 10, 15, 20, and 25 °C in 13CH 4-fed microcosms from two geographically distinct natural wetlands: Sanjiang Plain wetland in northeast China and Haibei wetland in Tibet Plateau. In both wetlands, CH 4 oxidation potential was enhanced with increasing temperature. Community profiling revealed that type I methanotrophs dominated CH 4 oxidation, although a small portion (2.76%–17.14%) of type II methanotrophs (Methylocystis, Methylosinus/Methylocystis) were significantly stimulated at 20 °C and 25 °C. 13C-labeled indicator species included Methylobacter, Methylocystis, and Methylosarcina species in Sanjiang Plain, and Methylobacter and Methylosarcina species in Haibei. Network analysis demonstrated positive co-occurrence of species between genera of Methylobacter, Methylosarcina, and Methylocystis with shifts in temperature, while interspecies interactions between Methylobacter and Methylomonas correlated negatively, and Methylobacter and Methylosinus/Methylocystis positively. Partial least squares path modeling illustrated that the direct effects of temperature on CH 4 oxidation were stronger in northeast China than Tibet Plateau, and temperature could also indirectly influence CH 4 oxidation via shifts in the methanotroph communities. Collectively, these results provide insights into how temperature could influence methanotrophy in natural wetlands under future climate scenarios.
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- Faculties (pre 2018 reorg) > Faculty of Engineering and the Environment (pre 2018 reorg) > Southampton Marine & Maritime Institute (pre 2018 reorg)
- Current Faculties > Faculty of Environmental and Life Sciences > School of Biological Sciences > Microbiology
School of Biological Sciences > Microbiology - Faculties (pre 2018 reorg) > Faculty of Natural and Environmental Sciences (pre 2018 reorg) > Institute for Life Sciences (pre 2018 reorg)
Current Faculties > Faculty of Environmental and Life Sciences > Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg)
Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg)
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