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Silver or gold? A comparison of nanoparticle modified electrochemical genosensors based on cobalt porphyrin-DNA

Silver or gold? A comparison of nanoparticle modified electrochemical genosensors based on cobalt porphyrin-DNA
Silver or gold? A comparison of nanoparticle modified electrochemical genosensors based on cobalt porphyrin-DNA
We applied a cobalt-porphyrin modified DNA as electrochemical marker, which was attached to nanoparticles, to detect specific DNA sequences. We compare the performance of gold and silver NPs in oligonucleotide sensors to determine if a change in metal will lead to either higher sensitivity or different selectivity, based on the redox behaviour of silver vs. gold. Surprisingly, we find that using either gold or silver NPs yields very similar overall performance. The electrochemical measurements of both types of sensors show the same redox behaviour which is dominated by the cobalt porphyrin, indicating that the electron pathway does not include the NP, but there is direct electron transfer between the porphyrin and the electrode. Both sensors show a linear response in the range of 5 × 10–17 to 1 × 10–16 M; the limit of detection (LOD) is 3.8 × 10–18 M for the AuNP sensor, and 5.0 × 10–18 M for the AgNP sensor, respectively, which corresponds to the detection of about 20–50 DNA molecules in the analyte. Overall, the silver system results in a better DNA economy and using cheaper starting materials for the NPs, thus shows better cost-effectiveness and could be more suitable for the mass-production of highly sensitive DNA sensors.
Silver nanoparticles, Gold nanoparticles, Cobalt porphyrin, Electrochemical genosensor, Ultrasensitive detection
1567-5394
Malecka, Kamila
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Kaur, Balwinder
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Cristaldi, D. Andrea
6da2333e-3305-4a8b-996f-e5a844c69cdc
Chay, Clarissa S.
0b134968-23f9-4c88-9fb8-226703b3ec09
Mames, Iwona
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Radecka, Hanna
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Radecki, Jerzy
8bb31f8a-1c39-48f3-805a-4343740a9e8e
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d
Malecka, Kamila
2b1d9c1a-03fc-4172-b0a0-87f0342d8635
Kaur, Balwinder
7d4a14ab-cb48-482f-aac2-57d453a9fa63
Cristaldi, D. Andrea
6da2333e-3305-4a8b-996f-e5a844c69cdc
Chay, Clarissa S.
0b134968-23f9-4c88-9fb8-226703b3ec09
Mames, Iwona
c7177d38-bda3-44d2-9140-9cfb9624ca49
Radecka, Hanna
e103c3ea-c929-4858-8eee-aa1723a58cb2
Radecki, Jerzy
8bb31f8a-1c39-48f3-805a-4343740a9e8e
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d

Malecka, Kamila, Kaur, Balwinder, Cristaldi, D. Andrea, Chay, Clarissa S., Mames, Iwona, Radecka, Hanna, Radecki, Jerzy and Stulz, Eugen (2020) Silver or gold? A comparison of nanoparticle modified electrochemical genosensors based on cobalt porphyrin-DNA. Bioelectrochemistry, 138, [107723]. (doi:10.1016/j.bioelechem.2020.107723).

Record type: Article

Abstract

We applied a cobalt-porphyrin modified DNA as electrochemical marker, which was attached to nanoparticles, to detect specific DNA sequences. We compare the performance of gold and silver NPs in oligonucleotide sensors to determine if a change in metal will lead to either higher sensitivity or different selectivity, based on the redox behaviour of silver vs. gold. Surprisingly, we find that using either gold or silver NPs yields very similar overall performance. The electrochemical measurements of both types of sensors show the same redox behaviour which is dominated by the cobalt porphyrin, indicating that the electron pathway does not include the NP, but there is direct electron transfer between the porphyrin and the electrode. Both sensors show a linear response in the range of 5 × 10–17 to 1 × 10–16 M; the limit of detection (LOD) is 3.8 × 10–18 M for the AuNP sensor, and 5.0 × 10–18 M for the AgNP sensor, respectively, which corresponds to the detection of about 20–50 DNA molecules in the analyte. Overall, the silver system results in a better DNA economy and using cheaper starting materials for the NPs, thus shows better cost-effectiveness and could be more suitable for the mass-production of highly sensitive DNA sensors.

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Silver or Gold - Accepted Manuscript
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Accepted/In Press date: 5 December 2020
e-pub ahead of print date: 14 December 2020
Keywords: Silver nanoparticles, Gold nanoparticles, Cobalt porphyrin, Electrochemical genosensor, Ultrasensitive detection

Identifiers

Local EPrints ID: 445722
URI: http://eprints.soton.ac.uk/id/eprint/445722
ISSN: 1567-5394
PURE UUID: 19b0f013-2d84-4053-a0b9-77d17d93ccc3
ORCID for Eugen Stulz: ORCID iD orcid.org/0000-0002-5302-2276

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Date deposited: 06 Jan 2021 17:42
Last modified: 15 Sep 2021 01:51

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