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Acoustofluidic phase microscopy in a tilted segmentation-free configuration

Acoustofluidic phase microscopy in a tilted segmentation-free configuration
Acoustofluidic phase microscopy in a tilted segmentation-free configuration

A low-cost device for registration-free quantitative phase microscopy (QPM) based on the transport of intensity equation of cells in continuous flow is presented. The method uses acoustic focusing to align cells into a single plane where all cells move at a constant speed. The acoustic focusing plane is tilted with respect to the microscope's focal plane in order to obtain cell images at multiple focal positions. As the cells are displaced at constant speed, phase maps can be generated without the need to segment and register individual objects. The proposed inclined geometry allows for the acquisition of a vertical stack without the need for any moving part, and it enables a cost-effective and robust implementation of QPM. The suitability of the solution for biological imaging is tested on blood samples, demonstrating the ability to recover the phase map of single red blood cells flowing through the microchip.

1932-1058
Morales, J.
394744c6-a1b3-4808-8e04-f501f427a35f
Hammarstrom, Bjorn
e68f865f-bb5e-4170-bc91-cf7b4ea60ba3
Lippi, G.L.
de48876d-d99c-463c-8a58-aec29221d9f5
Vassalli, Massimo
e6b24b46-2d13-4d0f-98ac-b8670b33758a
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Morales, J.
394744c6-a1b3-4808-8e04-f501f427a35f
Hammarstrom, Bjorn
e68f865f-bb5e-4170-bc91-cf7b4ea60ba3
Lippi, G.L.
de48876d-d99c-463c-8a58-aec29221d9f5
Vassalli, Massimo
e6b24b46-2d13-4d0f-98ac-b8670b33758a
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d

Morales, J., Hammarstrom, Bjorn, Lippi, G.L., Vassalli, Massimo and Glynne-Jones, Peter (2021) Acoustofluidic phase microscopy in a tilted segmentation-free configuration. Biomicrofluidics, 15 (1), [014102]. (doi:10.1063/5.0036585).

Record type: Article

Abstract

A low-cost device for registration-free quantitative phase microscopy (QPM) based on the transport of intensity equation of cells in continuous flow is presented. The method uses acoustic focusing to align cells into a single plane where all cells move at a constant speed. The acoustic focusing plane is tilted with respect to the microscope's focal plane in order to obtain cell images at multiple focal positions. As the cells are displaced at constant speed, phase maps can be generated without the need to segment and register individual objects. The proposed inclined geometry allows for the acquisition of a vertical stack without the need for any moving part, and it enables a cost-effective and robust implementation of QPM. The suitability of the solution for biological imaging is tested on blood samples, demonstrating the ability to recover the phase map of single red blood cells flowing through the microchip.

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with_doi article_accepted_manuscript_393690_838130_2 - Accepted Manuscript
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Accepted/In Press date: 1 December 2020
e-pub ahead of print date: 5 January 2021
Published date: January 2021
Additional Information: Funding Information: The authors would like to thank Dr. Stéphane Barland (UCA Nice) and Dr. Marco Sartore (ElbaTech SRL) for fruitful discussions. J.M.M. acknowledges the funding for international mobility France–Italy provided by the Université Franco Italienne (UFI, Project No. C2-1031) and the Mexican Council of Science and Technology (CONACyT) scholarship (No. 471712). P.G.J. gratefully acknowledges fellowship funding by the UK EPSRC (No. EP/ L025035/1). This work has also been supported by the French government through the UCAJEDI Investments in the Future project managed by the National Research Agency (ANR) with Reference No. ANR-15-IDEX-01. Publisher Copyright: © 2021 Author(s).

Identifiers

Local EPrints ID: 445851
URI: http://eprints.soton.ac.uk/id/eprint/445851
ISSN: 1932-1058
PURE UUID: 34b4a67a-56ff-422d-9576-c63dd9b4c673
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953

Catalogue record

Date deposited: 11 Jan 2021 17:32
Last modified: 30 Jun 2022 01:37

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Contributors

Author: J. Morales
Author: Bjorn Hammarstrom
Author: G.L. Lippi
Author: Massimo Vassalli

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