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Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans

Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome, is one of the most destructive pathogens of freshwater fishes. To date, the disease has been reported from over 160 fish species in 20 countries and notably, this is the first non-salmonid disease that has resulted in major impacts globally. In particular, Indian major carps (IMCs) are highly susceptible to this disease. To increase our knowledge particularly with regards to host immune response against A. invadans infection in a susceptible host, the gene expression profile in head kidney of A. invadans-infected and control rohu, Labeo rohita was investigated using RNA sequencing. Time course analysis of RNA-Seq data revealed 5608 differentially expressed genes, involved among others in Antigen processing and presentation, Leukocyte transendothelial migration, IL-17 signaling, Chemokine signaling, C-type lectin receptor signaling and Toll-like receptor signaling pathways. In the affected pathways, a number of immune genes were found to be downregulated, suggesting an immune evasion strategy of A. invadans in establishing the infection. The information generated in this study offers first systematic mechanistic understanding of the host–pathogen interaction that might underpin the development of new management strategies for this economically devastating fish-pathogenic oomycete A. invadans.
2045-2322
Pradhan, P. K.
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Verma, Dev Kumar
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Peruzza, Luca
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Gupta, Shubham
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Haq, Syed Assim
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Shubin, Sergei V.
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Morgan, Kenton L.
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Trusch, Franziska
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Mohindra, Vindhya
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Hauton, Chris
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Van West, Pieter
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Sood, Neeraj
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Pradhan, P. K.
1764bc7f-8df4-41f2-a194-8022847b33c3
Verma, Dev Kumar
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Peruzza, Luca
97dfdd24-f9e8-4d76-aee5-67bdd9b92541
Gupta, Shubham
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Haq, Syed Assim
b8c60de7-f597-49b0-9fb8-fbfd17e4baa9
Shubin, Sergei V.
39a9db5b-5f62-4f3a-9836-1df6c902d46a
Morgan, Kenton L.
faba11e1-eb8a-4b45-b1e2-60b046b079ba
Trusch, Franziska
0ad6ffdf-be32-4f8b-9373-269da8b718fb
Mohindra, Vindhya
f07f2cc4-7038-476e-95b0-0938a266e87f
Hauton, Chris
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Van West, Pieter
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Sood, Neeraj
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Pradhan, P. K., Verma, Dev Kumar, Peruzza, Luca, Gupta, Shubham, Haq, Syed Assim, Shubin, Sergei V., Morgan, Kenton L., Trusch, Franziska, Mohindra, Vindhya, Hauton, Chris, Van West, Pieter and Sood, Neeraj (2020) Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans. Scientific Reports, 10 (1), [19531]. (doi:10.1038/s41598-020-76278-w).

Record type: Article

Abstract

Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome, is one of the most destructive pathogens of freshwater fishes. To date, the disease has been reported from over 160 fish species in 20 countries and notably, this is the first non-salmonid disease that has resulted in major impacts globally. In particular, Indian major carps (IMCs) are highly susceptible to this disease. To increase our knowledge particularly with regards to host immune response against A. invadans infection in a susceptible host, the gene expression profile in head kidney of A. invadans-infected and control rohu, Labeo rohita was investigated using RNA sequencing. Time course analysis of RNA-Seq data revealed 5608 differentially expressed genes, involved among others in Antigen processing and presentation, Leukocyte transendothelial migration, IL-17 signaling, Chemokine signaling, C-type lectin receptor signaling and Toll-like receptor signaling pathways. In the affected pathways, a number of immune genes were found to be downregulated, suggesting an immune evasion strategy of A. invadans in establishing the infection. The information generated in this study offers first systematic mechanistic understanding of the host–pathogen interaction that might underpin the development of new management strategies for this economically devastating fish-pathogenic oomycete A. invadans.

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s41598-020-76278-w - Version of Record
Available under License Creative Commons Attribution.
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Accepted/In Press date: 20 October 2020
Published date: 11 November 2020

Identifiers

Local EPrints ID: 445918
URI: http://eprints.soton.ac.uk/id/eprint/445918
ISSN: 2045-2322
PURE UUID: 53ebb478-604b-40bc-b973-d0a35a1c12b4
ORCID for Chris Hauton: ORCID iD orcid.org/0000-0002-2313-4226

Catalogue record

Date deposited: 13 Jan 2021 17:32
Last modified: 26 Nov 2021 02:38

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Contributors

Author: P. K. Pradhan
Author: Dev Kumar Verma
Author: Luca Peruzza
Author: Shubham Gupta
Author: Syed Assim Haq
Author: Sergei V. Shubin
Author: Kenton L. Morgan
Author: Franziska Trusch
Author: Vindhya Mohindra
Author: Chris Hauton ORCID iD
Author: Pieter Van West
Author: Neeraj Sood

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