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Developing a system for in vivo imaging of maize roots containing iodinated contrast media in soil using synchrotron XCT and XRF

Developing a system for in vivo imaging of maize roots containing iodinated contrast media in soil using synchrotron XCT and XRF
Developing a system for in vivo imaging of maize roots containing iodinated contrast media in soil using synchrotron XCT and XRF
Aims
We sought to develop a novel experimental system which enabled application of iodinated contrast media to in vivo plant roots intact in soil and was compatible with time-resolved synchrotron X-ray computed tomography imaging. The system was developed to overcome issues of low contrast to noise within X-ray computed tomography images of plant roots and soil environments, the latter of which can complicate image processing and result in the loss of anatomical information.
Methods
To demonstrate the efficacy of the system we use employ the novel use of both synchrotron X-ray computed tomography and synchrotron X-ray fluorescence mapping to capture the translocation of the contrast media through root vasculature into the leaves.
Results
With the application of contrast media we identify fluid flow in root vasculature and visualise anatomical features, which are otherwise often only observable in ex vivo microscopy, including: the xylem, metaxylem, pith, fibres in aerenchyma and leaf venation. We are also able to observe interactions between aerenchyma cross sectional area and solute transport in the root vasculature with depth.
Conclusions
Our novel system was capable of successfully delivering sufficient contrast media into root and leaf tissues such that anatomical features could be visualised and internal fluid transport observed. We propose that our system, could be used in future to study internal plant transport mechanisms and parameterise models for fluid flow in plants.
roots, maize, soil, x-ray computed tomography, iodinated contrast media, x-ray fluoresence mapping
0032-079X
Scotson, Callum Paul
47901c28-548c-41cc-9cbd-f0429a24c7cb
Van Veelen, Arjen
cb6f2c8b-4671-4836-88a0-3987fd2f2d67
Williams, Katherine
a13f30b4-2f53-4a14-ad38-c733923a6450
Koebernick, Nicolai
118c4e45-02d8-42da-84c8-8ee4fac140ad
Mckay Fletcher, Daniel
db06e7e0-69af-4fa2-89b3-26f6599e43d4
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Scotson, Callum Paul
47901c28-548c-41cc-9cbd-f0429a24c7cb
Van Veelen, Arjen
cb6f2c8b-4671-4836-88a0-3987fd2f2d67
Williams, Katherine
a13f30b4-2f53-4a14-ad38-c733923a6450
Koebernick, Nicolai
118c4e45-02d8-42da-84c8-8ee4fac140ad
Mckay Fletcher, Daniel
db06e7e0-69af-4fa2-89b3-26f6599e43d4
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe

Scotson, Callum Paul, Van Veelen, Arjen, Williams, Katherine, Koebernick, Nicolai, Mckay Fletcher, Daniel and Roose, Tiina (2020) Developing a system for in vivo imaging of maize roots containing iodinated contrast media in soil using synchrotron XCT and XRF. Plant and Soil. (doi:10.1007/s11104-020-04784-x).

Record type: Article

Abstract

Aims
We sought to develop a novel experimental system which enabled application of iodinated contrast media to in vivo plant roots intact in soil and was compatible with time-resolved synchrotron X-ray computed tomography imaging. The system was developed to overcome issues of low contrast to noise within X-ray computed tomography images of plant roots and soil environments, the latter of which can complicate image processing and result in the loss of anatomical information.
Methods
To demonstrate the efficacy of the system we use employ the novel use of both synchrotron X-ray computed tomography and synchrotron X-ray fluorescence mapping to capture the translocation of the contrast media through root vasculature into the leaves.
Results
With the application of contrast media we identify fluid flow in root vasculature and visualise anatomical features, which are otherwise often only observable in ex vivo microscopy, including: the xylem, metaxylem, pith, fibres in aerenchyma and leaf venation. We are also able to observe interactions between aerenchyma cross sectional area and solute transport in the root vasculature with depth.
Conclusions
Our novel system was capable of successfully delivering sufficient contrast media into root and leaf tissues such that anatomical features could be visualised and internal fluid transport observed. We propose that our system, could be used in future to study internal plant transport mechanisms and parameterise models for fluid flow in plants.

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More information

Accepted/In Press date: 25 November 2020
e-pub ahead of print date: 10 December 2020
Keywords: roots, maize, soil, x-ray computed tomography, iodinated contrast media, x-ray fluoresence mapping

Identifiers

Local EPrints ID: 446022
URI: http://eprints.soton.ac.uk/id/eprint/446022
ISSN: 0032-079X
PURE UUID: c73f12a8-9724-4f11-88dd-e52b99c71edb
ORCID for Katherine Williams: ORCID iD orcid.org/0000-0001-6827-9261
ORCID for Daniel Mckay Fletcher: ORCID iD orcid.org/0000-0001-6569-2931
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 19 Jan 2021 17:31
Last modified: 26 Nov 2021 03:21

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Contributors

Author: Callum Paul Scotson
Author: Arjen Van Veelen
Author: Nicolai Koebernick
Author: Daniel Mckay Fletcher ORCID iD
Author: Tiina Roose ORCID iD

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