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Endothelial cells: Co-culture spheroids

Endothelial cells: Co-culture spheroids
Endothelial cells: Co-culture spheroids
The development and maintenance of a functioning vascular system is a critical function for many aspects of tissue growth and regeneration. Vascular endothelial cell in vitro co-culture spheroids are self-organized cell composites that have the capacity to recapitulate the three-dimensional tissue microenvironment. These spheroid testing platforms aim to better understand the mechanisms of functional tissue and how new therapeutic agents can drive these 3D co-culture processes. Here we describe direct cell–cell 3D endothelial co-culture spheroid methods, to examine the physiological spatial growth and cell–cell interaction of vascular cells and surrounding native tissue cells in the formation of vascular networks within spheroids and the potential to regenerate tissue.
47-56
Springer
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Wells, Julia A.
2a6c8f1a-1be9-4086-a3f2-117ffcf8a7f1
Oreffo, Richard O. C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Wells, Julia A.
2a6c8f1a-1be9-4086-a3f2-117ffcf8a7f1
Oreffo, Richard O. C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Kanczler, Janos M., Wells, Julia A. and Oreffo, Richard O. C. (2020) Endothelial cells: Co-culture spheroids. In, Vascular Morphogenesis Methods and Protocols part of the Methods in Molecular Biology book series. (Methods in Molecular Biology, 2206) Springer, pp. 47-56. (doi:10.1007/978-1-0716-0916-3_5).

Record type: Book Section

Abstract

The development and maintenance of a functioning vascular system is a critical function for many aspects of tissue growth and regeneration. Vascular endothelial cell in vitro co-culture spheroids are self-organized cell composites that have the capacity to recapitulate the three-dimensional tissue microenvironment. These spheroid testing platforms aim to better understand the mechanisms of functional tissue and how new therapeutic agents can drive these 3D co-culture processes. Here we describe direct cell–cell 3D endothelial co-culture spheroid methods, to examine the physiological spatial growth and cell–cell interaction of vascular cells and surrounding native tissue cells in the formation of vascular networks within spheroids and the potential to regenerate tissue.

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e-pub ahead of print date: 5 August 2020

Identifiers

Local EPrints ID: 446337
URI: http://eprints.soton.ac.uk/id/eprint/446337
PURE UUID: 2ed3af1e-9e03-4342-a334-43aee1536100
ORCID for Janos M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for Julia A. Wells: ORCID iD orcid.org/0000-0001-8272-0236
ORCID for Richard O. C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 04 Feb 2021 17:34
Last modified: 26 Nov 2021 02:57

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Contributors

Author: Janos M. Kanczler ORCID iD
Author: Julia A. Wells ORCID iD

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