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Epigenome-wide association of infant feeding and changes in DNA methylation from birth to 10 Years

Epigenome-wide association of infant feeding and changes in DNA methylation from birth to 10 Years
Epigenome-wide association of infant feeding and changes in DNA methylation from birth to 10 Years

Epigenetic factors have been suggested as mediators of early-life nutrition to future health. Prior studies focused on breastfeeding effects on DNA methylation (DNAm), ignoring other feeding modes. In this analysis of the Isle of Wight birth cohort, feeding modes were categorized as exclusive breastfeeding (EBF), exclusive formula feeding (EFF), and mixed feeding based on whether the respective feeding mode lasted for at least 3 months. In addition, in the past, infant feeding modes were assessed using DNAm at one time point in childhood, not changes of DNAm. In this paper, methylation differences (delta DNAm) were calculated by subtracting residual methylation values at birth from age 10 years (adjusting for cell types and season of blood collection at both ages). These deltas were estimated for all methylation sites where cytosine was followed by guanine (cytosine guanine dinucleotide (CpG) sites). Then, we performed an epigenome-wide association study contrasting EBF, EFF, and mixed feeding with delta DNAm that represents changes in methylation from birth to 10 years. A total of 87 CpGs (EBF: 27 CpGs, EFF: 48 CpGs, mixed: 12 CpGs) were identified using separate linear regression models adjusting for confounders and multiple testing. The sum of all changes in methylation from birth to age 10 years was significantly lower in the EFF group. Correspondingly, the number of CpGs with a methylation decline was 4.7% higher reflecting 13,683 CpGs. Lower methylation related to exclusive formula feeding and its adverse potential for the child's development needs future research to reduce adverse health effects.

2072-6643
Mallisetty, Yamini
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Mukherjee, Nandini
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Jiang, Yu
46412df7-7e41-4ab9-9d5a-e4d469c255cc
Chen, Su
1bcae119-70e2-4abf-817a-9d78e54ef946
Ewart, Susan
28667421-3cf7-43d7-b1c3-ca27564938f7
Arshad, S Hasan
917e246d-2e60-472f-8d30-94b01ef28958
Holloway, John W
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Zhang, Hongmei
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Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853
Mallisetty, Yamini
ead5ca51-5959-4a6e-a817-7b4bbf908006
Mukherjee, Nandini
f64f02d6-2fd0-40db-88ee-5f85b59b8e0b
Jiang, Yu
46412df7-7e41-4ab9-9d5a-e4d469c255cc
Chen, Su
1bcae119-70e2-4abf-817a-9d78e54ef946
Ewart, Susan
28667421-3cf7-43d7-b1c3-ca27564938f7
Arshad, S Hasan
917e246d-2e60-472f-8d30-94b01ef28958
Holloway, John W
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853

Mallisetty, Yamini, Mukherjee, Nandini, Jiang, Yu, Chen, Su, Ewart, Susan, Arshad, S Hasan, Holloway, John W, Zhang, Hongmei and Karmaus, Wilfried (2020) Epigenome-wide association of infant feeding and changes in DNA methylation from birth to 10 Years. Nutrients, 13 (1). (doi:10.3390/nu13010099).

Record type: Article

Abstract

Epigenetic factors have been suggested as mediators of early-life nutrition to future health. Prior studies focused on breastfeeding effects on DNA methylation (DNAm), ignoring other feeding modes. In this analysis of the Isle of Wight birth cohort, feeding modes were categorized as exclusive breastfeeding (EBF), exclusive formula feeding (EFF), and mixed feeding based on whether the respective feeding mode lasted for at least 3 months. In addition, in the past, infant feeding modes were assessed using DNAm at one time point in childhood, not changes of DNAm. In this paper, methylation differences (delta DNAm) were calculated by subtracting residual methylation values at birth from age 10 years (adjusting for cell types and season of blood collection at both ages). These deltas were estimated for all methylation sites where cytosine was followed by guanine (cytosine guanine dinucleotide (CpG) sites). Then, we performed an epigenome-wide association study contrasting EBF, EFF, and mixed feeding with delta DNAm that represents changes in methylation from birth to 10 years. A total of 87 CpGs (EBF: 27 CpGs, EFF: 48 CpGs, mixed: 12 CpGs) were identified using separate linear regression models adjusting for confounders and multiple testing. The sum of all changes in methylation from birth to age 10 years was significantly lower in the EFF group. Correspondingly, the number of CpGs with a methylation decline was 4.7% higher reflecting 13,683 CpGs. Lower methylation related to exclusive formula feeding and its adverse potential for the child's development needs future research to reduce adverse health effects.

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Epigenome-Wide Association of Infant Feeding - Version of Record
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Accepted/In Press date: 28 December 2020
Published date: 30 December 2020

Identifiers

Local EPrints ID: 446352
URI: http://eprints.soton.ac.uk/id/eprint/446352
ISSN: 2072-6643
PURE UUID: 7212d741-1dbf-4ca5-8339-5b0373e953f7
ORCID for John W Holloway: ORCID iD orcid.org/0000-0001-9998-0464

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Date deposited: 05 Feb 2021 17:30
Last modified: 26 Nov 2021 02:40

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Contributors

Author: Yamini Mallisetty
Author: Nandini Mukherjee
Author: Yu Jiang
Author: Su Chen
Author: Susan Ewart
Author: S Hasan Arshad
Author: John W Holloway ORCID iD
Author: Hongmei Zhang
Author: Wilfried Karmaus

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