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Is there an advantage in enriching parenteral lipid emulsions containing fatty acids from fish oil with medium-chain triglycerides? A study on body pool concentrations of omega-3 fatty acids in Lewis rats

Is there an advantage in enriching parenteral lipid emulsions containing fatty acids from fish oil with medium-chain triglycerides? A study on body pool concentrations of omega-3 fatty acids in Lewis rats
Is there an advantage in enriching parenteral lipid emulsions containing fatty acids from fish oil with medium-chain triglycerides? A study on body pool concentrations of omega-3 fatty acids in Lewis rats
Background: the addition of medium‐chain triglycerides (MCTs) into parenteral lipid emulsions rich in fatty acids from fish oil (FOLEs) has been shown to improve their clearance and extrahepatic uptake. We assessed whether this effect could favor the leukocyte uptake of ω‐3 polyunsaturated fatty acids (PUFAs) for immunomodulatory purposes Methods: following 5‐day adaptation in metabolic cages, 42 male Lewis rats fed with AIN‐93M chow were killed (baseline control group [BC]) or submitted to central venous catheterization and distributed into (1) surgical control group without parenteral infusion (chow group), (2) test emulsion (MCT/LCT/FO) group with the parenteral infusion of a FOLE containing 40% MCT, and (3) control emulsion group (LCT/FO) with the parenteral infusion of an FOLE without MCT. The 2 FOLEs had similar ω‐3 PUFA contents and ω‐6/ω‐3 PUFA ratios and were infused during 48 and 72 hours. Concentrations of ω‐3 and ω‐6 PUFAs in plasma, liver, and blood mononuclear and polymorphonuclear leukocytes were assessed by gas chromatography Results: in both FOLE groups, leukocyte concentrations of ω‐3 PUFAs peaked after 48 hours’ infusion (vs BC). At this time point, plasma concentrations of ω‐3 PUFAs were higher in MCT/LCT/FO group than in LCT/FO group and the opposite was found in the liver (P<.05), but no differences in PUFA concentrations were observed between these groups in leukocytes (P>.05) Conclusion: the ω‐3 PUFAs provided by FOLEs rich in MCT were less incorporated by liver and remained more available for extrahepatic cell delivery, but this did not result in a clear benefit in increasing their incorporation by peripheral leukocytes.
0148-6071
Tesser, A
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Torrinhas, RS
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Garla, PC
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Oliveira-Filho, RS
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Aprobato, FGG
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Tamanaha, EM
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Antunes, MS
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Sampaio, GR
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Torres, E
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Calder, Philip
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Waitzberg, DL
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Tesser, A
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Torrinhas, RS
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Garla, PC
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Oliveira-Filho, RS
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Aprobato, FGG
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Tamanaha, EM
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Antunes, MS
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Sampaio, GR
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Torres, E
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Calder, Philip
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Waitzberg, DL
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Tesser, A, Torrinhas, RS, Garla, PC, Oliveira-Filho, RS, Aprobato, FGG, Tamanaha, EM, Antunes, MS, Sampaio, GR, Torres, E, Calder, Philip and Waitzberg, DL (2020) Is there an advantage in enriching parenteral lipid emulsions containing fatty acids from fish oil with medium-chain triglycerides? A study on body pool concentrations of omega-3 fatty acids in Lewis rats. Journal of Parenteral and Enteral Nutrition. (doi:10.1002/jpen.2041).

Record type: Article

Abstract

Background: the addition of medium‐chain triglycerides (MCTs) into parenteral lipid emulsions rich in fatty acids from fish oil (FOLEs) has been shown to improve their clearance and extrahepatic uptake. We assessed whether this effect could favor the leukocyte uptake of ω‐3 polyunsaturated fatty acids (PUFAs) for immunomodulatory purposes Methods: following 5‐day adaptation in metabolic cages, 42 male Lewis rats fed with AIN‐93M chow were killed (baseline control group [BC]) or submitted to central venous catheterization and distributed into (1) surgical control group without parenteral infusion (chow group), (2) test emulsion (MCT/LCT/FO) group with the parenteral infusion of a FOLE containing 40% MCT, and (3) control emulsion group (LCT/FO) with the parenteral infusion of an FOLE without MCT. The 2 FOLEs had similar ω‐3 PUFA contents and ω‐6/ω‐3 PUFA ratios and were infused during 48 and 72 hours. Concentrations of ω‐3 and ω‐6 PUFAs in plasma, liver, and blood mononuclear and polymorphonuclear leukocytes were assessed by gas chromatography Results: in both FOLE groups, leukocyte concentrations of ω‐3 PUFAs peaked after 48 hours’ infusion (vs BC). At this time point, plasma concentrations of ω‐3 PUFAs were higher in MCT/LCT/FO group than in LCT/FO group and the opposite was found in the liver (P<.05), but no differences in PUFA concentrations were observed between these groups in leukocytes (P>.05) Conclusion: the ω‐3 PUFAs provided by FOLEs rich in MCT were less incorporated by liver and remained more available for extrahepatic cell delivery, but this did not result in a clear benefit in increasing their incorporation by peripheral leukocytes.

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Accepted/In Press date: 19 October 2020
e-pub ahead of print date: 27 October 2020

Identifiers

Local EPrints ID: 446520
URI: http://eprints.soton.ac.uk/id/eprint/446520
ISSN: 0148-6071
PURE UUID: d3c92cf4-d941-4421-8216-570477e0e39b
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 12 Feb 2021 17:30
Last modified: 26 Nov 2021 05:58

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Contributors

Author: A Tesser
Author: RS Torrinhas
Author: PC Garla
Author: RS Oliveira-Filho
Author: FGG Aprobato
Author: EM Tamanaha
Author: MS Antunes
Author: GR Sampaio
Author: E Torres
Author: Philip Calder ORCID iD
Author: DL Waitzberg

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