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Infusion time for fish oil-containing parenteral emulsions in surgery: A study on omega-3 fatty acid dynamics in rats

Infusion time for fish oil-containing parenteral emulsions in surgery: A study on omega-3 fatty acid dynamics in rats
Infusion time for fish oil-containing parenteral emulsions in surgery: A study on omega-3 fatty acid dynamics in rats
Objectives The aim of this study was to contribute to the design of specialized parenteral nutrition protocols in surgery by evaluating the dynamics of polyunsaturated fatty acid (PUFA) concentrations in different body pools after the infusion of fish oil–containing lipid emulsion (FOLE) in rats that had undergone surgical central venous catheterization (CVC). Methods After 5-d adaptation in metabolic cages, 78 male Lewis rats (300–450 g) fed a standard diet were sacrificed (baseline control) or had only CVC (surgical control) or also received a 72-h infusion of a parenteral lipid emulsion with or without fish oil. The catheterized animals were sacrificed 0 (T0), 2 (T2), 6 (T6), and 12 h (T12) after the infusion ended. Gas chromatography was used to determine the concentrations of eicosapentaenoic (EPA), docosahexaenoic (DHA), and arachidonic (ARA) acids and the ω-3 to ω-6 ratio in plasma, liver, and blood leukocytes. Kruskal–Wallis and Wilcoxon tests were applied to plasma and liver data and descriptive analysis to leukocyte data. Results Plasma, liver, and leukocytes exhibited almost undetectable EPA and DHA and detectable ARA concentrations at baseline. Immediately after FOLE infusion (T0), these PUFAs changed in all pools, resulting in a higher ratio of ω-3 to ω-6 compared with rats with no FOLE infusion (P < 0.05). All these changes decreased over time, with residual effects remaining until T6 in plasma, T12 in liver, and only until T2 in leukocytes. Conclusion Data from this study suggest that ω-3 PUFAs are cleared early after the end of FOLE infusion, mainly in leukocytes. This should be considered when FOLEs are applied for immunomodulatory purposes in surgery.
0899-9007
7
Antunes, MS
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Waitzberg, DL
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Tesser, A
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Aprobato, F
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Tamanaha, EM
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Oliveira, R
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Sampaio, GR
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Torres, E
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Garla, PC
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Calder, Philip
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Torrinhas, RS
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Antunes, MS
45e878fa-f509-4bca-850f-f281a9efb76a
Waitzberg, DL
f0d28f62-81c3-41b8-9387-dcf81d770445
Tesser, A
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Aprobato, F
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Tamanaha, EM
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Oliveira, R
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Sampaio, GR
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Torres, E
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Garla, PC
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Calder, Philip
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Torrinhas, RS
cc13764e-7621-439f-9998-128f21c4361a

Antunes, MS, Waitzberg, DL, Tesser, A, Aprobato, F, Tamanaha, EM, Oliveira, R, Sampaio, GR, Torres, E, Garla, PC, Calder, Philip and Torrinhas, RS (2020) Infusion time for fish oil-containing parenteral emulsions in surgery: A study on omega-3 fatty acid dynamics in rats. Nutrition, 83, 7, [111066]. (doi:10.1016/j.nut.2020.111066).

Record type: Article

Abstract

Objectives The aim of this study was to contribute to the design of specialized parenteral nutrition protocols in surgery by evaluating the dynamics of polyunsaturated fatty acid (PUFA) concentrations in different body pools after the infusion of fish oil–containing lipid emulsion (FOLE) in rats that had undergone surgical central venous catheterization (CVC). Methods After 5-d adaptation in metabolic cages, 78 male Lewis rats (300–450 g) fed a standard diet were sacrificed (baseline control) or had only CVC (surgical control) or also received a 72-h infusion of a parenteral lipid emulsion with or without fish oil. The catheterized animals were sacrificed 0 (T0), 2 (T2), 6 (T6), and 12 h (T12) after the infusion ended. Gas chromatography was used to determine the concentrations of eicosapentaenoic (EPA), docosahexaenoic (DHA), and arachidonic (ARA) acids and the ω-3 to ω-6 ratio in plasma, liver, and blood leukocytes. Kruskal–Wallis and Wilcoxon tests were applied to plasma and liver data and descriptive analysis to leukocyte data. Results Plasma, liver, and leukocytes exhibited almost undetectable EPA and DHA and detectable ARA concentrations at baseline. Immediately after FOLE infusion (T0), these PUFAs changed in all pools, resulting in a higher ratio of ω-3 to ω-6 compared with rats with no FOLE infusion (P < 0.05). All these changes decreased over time, with residual effects remaining until T6 in plasma, T12 in liver, and only until T2 in leukocytes. Conclusion Data from this study suggest that ω-3 PUFAs are cleared early after the end of FOLE infusion, mainly in leukocytes. This should be considered when FOLEs are applied for immunomodulatory purposes in surgery.

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Accepted/In Press date: 2 November 2020
e-pub ahead of print date: 19 November 2020

Identifiers

Local EPrints ID: 446700
URI: http://eprints.soton.ac.uk/id/eprint/446700
ISSN: 0899-9007
PURE UUID: 22398aa9-001f-4a93-b664-f88d771ee697
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 18 Feb 2021 17:31
Last modified: 26 Nov 2021 05:58

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Contributors

Author: MS Antunes
Author: DL Waitzberg
Author: A Tesser
Author: F Aprobato
Author: EM Tamanaha
Author: R Oliveira
Author: GR Sampaio
Author: E Torres
Author: PC Garla
Author: Philip Calder ORCID iD
Author: RS Torrinhas

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