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Neuropeptide Y is important for basal and seizure induced precursor cell proliferation in the hippocampus

Neuropeptide Y is important for basal and seizure induced precursor cell proliferation in the hippocampus
Neuropeptide Y is important for basal and seizure induced precursor cell proliferation in the hippocampus
We have shown that neuropeptide Y (NPY) regulates neurogenesis in the normal dentate gyrus (DG) via Y(1) receptors (Howell, O.W., Scharfman, H.E., Herzog, H., Sundstrom, L.E., Beck-Sickinger, A. and Gray, W.P. (2003) Neuropeptide Y is neuroproliferative for post-natal hippocampal precursor cells. J Neurochem, 86, 646-659; Howell, O.W., Doyle, K., Goodman, J.H., Scharfman, H.E., Herzog, H., Pringle, A., Beck-Sickinger, A.G. and Gray, W.P. (2005) Neuropeptide Y stimulates neuronal precursor proliferation in the post-natal and adult dentate gyrus. J Neurochem, 93, 560-570). This regulation may be relevant to epilepsy, because seizures increase both NPY expression and precursor cell proliferation in the DG. Therefore, the effects of NPY on DG precursors were evaluated in normal conditions and after status epilepticus. In addition, potentially distinct NPY-responsive precursors were identified, and an analysis performed not only of the DG, but also the caudal subventricular zone (cSVZ) and subcallosal zone (SCZ) where seizures modulate glial precursors. We show a proliferative effect of NPY on multipotent nestin cells expressing the stem cell marker Lewis-X from both the DG and the cSVZ/SCZ in vitro. We confirm an effect on proliferation in the cSVZ/SCZ of Y(1) receptor(-/-) mice and demonstrate a significant reduction in basal and seizure-induced proliferation in the DG of NPY(-/-) mice.
npy, neurogenesis, progenitor cell, stem cell, epilepsy, cell culture, nestin, subventricular zone, subcallosal zone, dentate gyrus
0969-9961
174-188
Howell, Owain W.
59b875da-844e-4738-9b3b-b9796cf3b21f
Silva, Sharmalene
73c236bb-924c-4257-b3d9-aacbaf9d9cd0
Scharfman, Helen E.
f8c4a6d3-eb2a-4d52-93ef-7609ef4fbb9d
Sosunov, Alexander A.
47079e88-e067-4bc8-b731-f8c624308030
Zaben, Malik
6390c24c-4458-486d-9188-3afda5c91359
Shatya, Anan
633f6784-c8aa-4590-a7ed-60e77a81acce
McKhann II, Guy
594acadf-307d-4c7c-a0b8-21c28b4198aa
Herzog, Herbert
2755435a-82d7-45d0-b1db-907f0a650302
Laskowski, Alexandra
f06f47d0-8587-4074-91b6-eb59f28572ae
Gray, William P.
f34a0e23-3cba-4b0a-8676-a1b2c3e4c095
Howell, Owain W.
59b875da-844e-4738-9b3b-b9796cf3b21f
Silva, Sharmalene
73c236bb-924c-4257-b3d9-aacbaf9d9cd0
Scharfman, Helen E.
f8c4a6d3-eb2a-4d52-93ef-7609ef4fbb9d
Sosunov, Alexander A.
47079e88-e067-4bc8-b731-f8c624308030
Zaben, Malik
6390c24c-4458-486d-9188-3afda5c91359
Shatya, Anan
633f6784-c8aa-4590-a7ed-60e77a81acce
McKhann II, Guy
594acadf-307d-4c7c-a0b8-21c28b4198aa
Herzog, Herbert
2755435a-82d7-45d0-b1db-907f0a650302
Laskowski, Alexandra
f06f47d0-8587-4074-91b6-eb59f28572ae
Gray, William P.
f34a0e23-3cba-4b0a-8676-a1b2c3e4c095

Howell, Owain W., Silva, Sharmalene, Scharfman, Helen E., Sosunov, Alexander A., Zaben, Malik, Shatya, Anan, McKhann II, Guy, Herzog, Herbert, Laskowski, Alexandra and Gray, William P. (2007) Neuropeptide Y is important for basal and seizure induced precursor cell proliferation in the hippocampus. Neurobiology of Disease, 26 (1), 174-188. (doi:10.1016/j.nbd.2006.12.014).

Record type: Article

Abstract

We have shown that neuropeptide Y (NPY) regulates neurogenesis in the normal dentate gyrus (DG) via Y(1) receptors (Howell, O.W., Scharfman, H.E., Herzog, H., Sundstrom, L.E., Beck-Sickinger, A. and Gray, W.P. (2003) Neuropeptide Y is neuroproliferative for post-natal hippocampal precursor cells. J Neurochem, 86, 646-659; Howell, O.W., Doyle, K., Goodman, J.H., Scharfman, H.E., Herzog, H., Pringle, A., Beck-Sickinger, A.G. and Gray, W.P. (2005) Neuropeptide Y stimulates neuronal precursor proliferation in the post-natal and adult dentate gyrus. J Neurochem, 93, 560-570). This regulation may be relevant to epilepsy, because seizures increase both NPY expression and precursor cell proliferation in the DG. Therefore, the effects of NPY on DG precursors were evaluated in normal conditions and after status epilepticus. In addition, potentially distinct NPY-responsive precursors were identified, and an analysis performed not only of the DG, but also the caudal subventricular zone (cSVZ) and subcallosal zone (SCZ) where seizures modulate glial precursors. We show a proliferative effect of NPY on multipotent nestin cells expressing the stem cell marker Lewis-X from both the DG and the cSVZ/SCZ in vitro. We confirm an effect on proliferation in the cSVZ/SCZ of Y(1) receptor(-/-) mice and demonstrate a significant reduction in basal and seizure-induced proliferation in the DG of NPY(-/-) mice.

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More information

Published date: April 2007
Keywords: npy, neurogenesis, progenitor cell, stem cell, epilepsy, cell culture, nestin, subventricular zone, subcallosal zone, dentate gyrus

Identifiers

Local EPrints ID: 44674
URI: http://eprints.soton.ac.uk/id/eprint/44674
ISSN: 0969-9961
PURE UUID: c4747f5a-fcd3-4ebc-a15b-26420df65ef3

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Date deposited: 08 Mar 2007
Last modified: 15 Mar 2024 09:06

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Contributors

Author: Owain W. Howell
Author: Sharmalene Silva
Author: Helen E. Scharfman
Author: Alexander A. Sosunov
Author: Malik Zaben
Author: Anan Shatya
Author: Guy McKhann II
Author: Herbert Herzog
Author: Alexandra Laskowski
Author: William P. Gray

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