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Generation of mast cells from murine stem cell progenitors

Generation of mast cells from murine stem cell progenitors
Generation of mast cells from murine stem cell progenitors
Mouse bone marrow-derived mast cells (mBMMCs) are an invaluable tool for the study of mast cell function as they represent a primary source of mature mast cells. They can be sourced from wild-type, knockout, and transgenic mice and are used to repopulate mast cell-deficient mice. This method describes the isolation of mast cell hematopoietic progenitors from the bone marrow of mouse femurs and their subsequent culture in an IL-3-rich culture medium. After 4 weeks in culture, mBMMCs are obtained in high number and are of high purity. Assessment of their granularity by toluidine staining and IgE receptor expression by flow cytometry is also described. These cells are a useful tool in the determination of in vitro and in vivo mast cell function in innate and adaptive immunity.
85-89
Humana Press/Springer
Swindle, Emily J.
fe393c7a-a513-4de4-b02e-27369bd7e84f
Swindle, Emily J.
fe393c7a-a513-4de4-b02e-27369bd7e84f

Swindle, Emily J. (2020) Generation of mast cells from murine stem cell progenitors. In, Basophils and Mast Cells: Methods and Protocols. Humana Press/Springer, pp. 85-89. (doi:10.1007/978-1-0716-0696-4_7).

Record type: Book Section

Abstract

Mouse bone marrow-derived mast cells (mBMMCs) are an invaluable tool for the study of mast cell function as they represent a primary source of mature mast cells. They can be sourced from wild-type, knockout, and transgenic mice and are used to repopulate mast cell-deficient mice. This method describes the isolation of mast cell hematopoietic progenitors from the bone marrow of mouse femurs and their subsequent culture in an IL-3-rich culture medium. After 4 weeks in culture, mBMMCs are obtained in high number and are of high purity. Assessment of their granularity by toluidine staining and IgE receptor expression by flow cytometry is also described. These cells are a useful tool in the determination of in vitro and in vivo mast cell function in innate and adaptive immunity.

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e-pub ahead of print date: 7 August 2020

Identifiers

Local EPrints ID: 446883
URI: http://eprints.soton.ac.uk/id/eprint/446883
PURE UUID: 0a604c6a-649e-40eb-aa3b-e389af68ef8e
ORCID for Emily J. Swindle: ORCID iD orcid.org/0000-0003-3644-7747

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Date deposited: 25 Feb 2021 17:30
Last modified: 26 Nov 2021 02:54

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