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Combination of the probiotics Lacticaseibacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis, BB-12 has limited effect on biomarkers of immunity and inflammation in older people resident in care homes: results from the Probiotics to Reduce Infections iN CarE home reSidentS (PRINCESS) randomised, controlled trial

Combination of the probiotics Lacticaseibacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis, BB-12 has limited effect on biomarkers of immunity and inflammation in older people resident in care homes: results from the Probiotics to Reduce Infections iN CarE home reSidentS (PRINCESS) randomised, controlled trial
Combination of the probiotics Lacticaseibacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis, BB-12 has limited effect on biomarkers of immunity and inflammation in older people resident in care homes: results from the Probiotics to Reduce Infections iN CarE home reSidentS (PRINCESS) randomised, controlled trial
Aging is associated with a decline in many components of the immune system (immunosenescence). Probiotics may improve the immune response in older people. The objective was to determine the effect of the combination of two probiotic organisms Lacticaseibacillus (previously known as Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis subsp. lactis, BB-12 (BB-12)] on a range of immune biomarkers measured in the blood of older people resident in care homes in the UK. In a randomized controlled trial, older people [aged 67-97 (mean 86) years] resident in care homes received the combination of LGG+BB-12 (1.3-1.6 × 10-9 CFU per day) or placebo for up to 12 months. Full blood count, blood immune cell phenotypes, plasma immune mediator concentrations, phagocytosis, and blood culture responses to immune stimulation were all measured. Response to seasonal influenza vaccination was measured in a subset of participants. Paired samples (i.e., before and after intervention) were available for 30 participants per group. LGG and BB-12 were more likely to be present in feces in the probiotic group and were present at higher numbers. There was no significant effect of the probiotics on components of the full blood count, blood immune cell phenotypes, plasma immune mediator concentrations, phagocytosis by neutrophils and monocytes, and blood culture responses to immune stimulation. There was an indication that the probiotics improved the response to seasonal influenza vaccination with significantly (p = 0.04) higher seroconversion to the A/Michigan/2015 vaccine strain in the probiotic group than in the placebo group (47 vs. 15%).
aging, care home residents, immunity, immunosenescence, inflammageing, inflammation, probiotic
1664-3224
Castro Herrera, Vivian Maritza
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Fisk, Helena Lucy
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Wootton, Mandy
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Lown, Mark
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Owen-Jones, Eleri
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Lau, Mandy
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Lowe, Rachel
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Hood, Kerenza
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Gillespie, David
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Hobbs, Richard
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Little, Paul
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Butler, Christopher C.
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Miles, Elizabeth
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Calder, Philip
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Castro Herrera, Vivian Maritza
ee06e5c1-815f-497f-9c67-47ead3772704
Fisk, Helena Lucy
2483d346-75dd-41b3-a481-10f8bb39cd9f
Wootton, Mandy
305ac6f7-9946-4996-a100-e692ed344760
Lown, Mark
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Owen-Jones, Eleri
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Lau, Mandy
ebd931bb-22d7-481b-ba04-f831f4e45cb4
Lowe, Rachel
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Hood, Kerenza
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Gillespie, David
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Hobbs, Richard
2a73b554-fadd-445c-a7f3-8e203a8cd9ce
Little, Paul
1bf2d1f7-200c-47a5-ab16-fe5a8756a777
Butler, Christopher C.
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Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
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Castro Herrera, Vivian Maritza, Fisk, Helena Lucy, Wootton, Mandy, Lown, Mark, Owen-Jones, Eleri, Lau, Mandy, Lowe, Rachel, Hood, Kerenza, Gillespie, David, Hobbs, Richard, Little, Paul, Butler, Christopher C., Miles, Elizabeth and Calder, Philip (2021) Combination of the probiotics Lacticaseibacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis, BB-12 has limited effect on biomarkers of immunity and inflammation in older people resident in care homes: results from the Probiotics to Reduce Infections iN CarE home reSidentS (PRINCESS) randomised, controlled trial. Frontiers in Immunology, 12, [643321]. (doi:10.3389/fimmu.2021.643321).

Record type: Article

Abstract

Aging is associated with a decline in many components of the immune system (immunosenescence). Probiotics may improve the immune response in older people. The objective was to determine the effect of the combination of two probiotic organisms Lacticaseibacillus (previously known as Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis subsp. lactis, BB-12 (BB-12)] on a range of immune biomarkers measured in the blood of older people resident in care homes in the UK. In a randomized controlled trial, older people [aged 67-97 (mean 86) years] resident in care homes received the combination of LGG+BB-12 (1.3-1.6 × 10-9 CFU per day) or placebo for up to 12 months. Full blood count, blood immune cell phenotypes, plasma immune mediator concentrations, phagocytosis, and blood culture responses to immune stimulation were all measured. Response to seasonal influenza vaccination was measured in a subset of participants. Paired samples (i.e., before and after intervention) were available for 30 participants per group. LGG and BB-12 were more likely to be present in feces in the probiotic group and were present at higher numbers. There was no significant effect of the probiotics on components of the full blood count, blood immune cell phenotypes, plasma immune mediator concentrations, phagocytosis by neutrophils and monocytes, and blood culture responses to immune stimulation. There was an indication that the probiotics improved the response to seasonal influenza vaccination with significantly (p = 0.04) higher seroconversion to the A/Michigan/2015 vaccine strain in the probiotic group than in the placebo group (47 vs. 15%).

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Accepted/In Press date: 12 February 2021
e-pub ahead of print date: 4 March 2021
Published date: 4 March 2021
Additional Information: Funding Information: This research was supported by the Efficacy and Mechanism Evaluation Programme which was funded by the Medical Research Council (MRC) and National Institute for Health Research (NIHR), with contributions from CSO in Scotland, HCRW in Wales and the HSC R&D, Public Health Agency in Northern Ireland. This project was managed by the NIHR Evaluation, Trials and Studies Coordination Centre (NETSCC) [Efficacy and Mechanism Evaluation (EME), grant number 13/95/10 – Probiotics to Reduce Infections iN CarE home reSidentS (PRINCESS)]. The Centre for Trial Research, Cardiff University was funded by Health & Care Research Wales and Cancer Research UK. VC-H was supported by Colciencias, Colombia. FH acknowledges part-funding from the NIHR School for Primary Care Research, the NIHR Collaboration for Leadership in Health Research and Care (CLARHC) Oxford, the NIHR Oxford Biomedical Research Centre, and the NIHR Oxford Medtech and in-vitro Diagnostics Co-operative. PC was supported by the NIHR Southampton Biomedical Research Centre. Publisher Copyright: © Copyright © 2021 Castro-Herrera, Fisk, Wootton, Lown, Owen-Jones, Lau, Lowe, Hood, Gillespie, Hobbs, Little, Butler, Miles and Calder.
Keywords: aging, care home residents, immunity, immunosenescence, inflammageing, inflammation, probiotic

Identifiers

Local EPrints ID: 447056
URI: http://eprints.soton.ac.uk/id/eprint/447056
ISSN: 1664-3224
PURE UUID: 4f39b89f-10b9-4c85-858c-44ff4cac5068
ORCID for Helena Lucy Fisk: ORCID iD orcid.org/0000-0002-9534-3246
ORCID for Mark Lown: ORCID iD orcid.org/0000-0001-8309-568X
ORCID for Paul Little: ORCID iD orcid.org/0000-0003-3664-1873
ORCID for Elizabeth Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 02 Mar 2021 17:31
Last modified: 12 Jul 2024 04:04

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Contributors

Author: Vivian Maritza Castro Herrera
Author: Helena Lucy Fisk ORCID iD
Author: Mandy Wootton
Author: Mark Lown ORCID iD
Author: Eleri Owen-Jones
Author: Mandy Lau
Author: Rachel Lowe
Author: Kerenza Hood
Author: David Gillespie
Author: Richard Hobbs
Author: Paul Little ORCID iD
Author: Christopher C. Butler
Author: Elizabeth Miles ORCID iD
Author: Philip Calder ORCID iD

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