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A DNA sensor based on upconversion nanoparticles and two-dimensional dichalcogenide materials

A DNA sensor based on upconversion nanoparticles and two-dimensional dichalcogenide materials
A DNA sensor based on upconversion nanoparticles and two-dimensional dichalcogenide materials

We demonstrate the fabrication of a new DNA sensor that is based on the optical interactions occurring between oligonucleotide-coated NaYF4:Yb3+;Er3+ upconversion nanoparticles and the two-dimensional dichalcogenide materials, MoS2 and WS2. Monodisperse upconversion nanoparticles were functionalized with single-stranded DNA endowing the nanoparticles with the ability to interact with the surface of the two-dimensional materials via van der Waals interactions leading to subsequent quenching of the upconversion fluorescence. By contrast, in the presence of a complementary oligonucleotide target and the formation of double-stranded DNA, the upconversion nanoparticles could not interact with MoS2 and WS2, thus retaining their inherent fluorescence properties. Utilizing this sensor we were able to detect target oligonucleotides with high sensitivity and specificity whilst reaching a concentration detection limit as low as 5 mol·L−1, within minutes.

[Figure not available: see fulltext.]

DNA sensor, MoS, WS, two-dimensional materials, upconversion nanoparticles
2095-0179
935-943
Alexaki, Konstantina
4eb3b612-8a61-41cc-855e-7ccf917310f7
Giust, Davide
fc70a34b-ee5e-4902-98b5-59cbb0e4ba7e
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
El-Sagheer, Afaf H.
0bf56d90-78ee-4772-add8-453ccacc39be
Brown, Tom
1cd7df32-b945-4ca1-8b59-a51a30191472
Muskens, Otto
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Alexaki, Konstantina
4eb3b612-8a61-41cc-855e-7ccf917310f7
Giust, Davide
fc70a34b-ee5e-4902-98b5-59cbb0e4ba7e
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
El-Sagheer, Afaf H.
0bf56d90-78ee-4772-add8-453ccacc39be
Brown, Tom
1cd7df32-b945-4ca1-8b59-a51a30191472
Muskens, Otto
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7

Alexaki, Konstantina, Giust, Davide, Kyriazi, Maria-Eleni, El-Sagheer, Afaf H., Brown, Tom, Muskens, Otto and Kanaras, Antonios (2021) A DNA sensor based on upconversion nanoparticles and two-dimensional dichalcogenide materials. Frontiers of Chemical Science and Engineering, 15 (4), 935-943. (doi:10.1007/s11705-020-2023-9).

Record type: Article

Abstract

We demonstrate the fabrication of a new DNA sensor that is based on the optical interactions occurring between oligonucleotide-coated NaYF4:Yb3+;Er3+ upconversion nanoparticles and the two-dimensional dichalcogenide materials, MoS2 and WS2. Monodisperse upconversion nanoparticles were functionalized with single-stranded DNA endowing the nanoparticles with the ability to interact with the surface of the two-dimensional materials via van der Waals interactions leading to subsequent quenching of the upconversion fluorescence. By contrast, in the presence of a complementary oligonucleotide target and the formation of double-stranded DNA, the upconversion nanoparticles could not interact with MoS2 and WS2, thus retaining their inherent fluorescence properties. Utilizing this sensor we were able to detect target oligonucleotides with high sensitivity and specificity whilst reaching a concentration detection limit as low as 5 mol·L−1, within minutes.

[Figure not available: see fulltext.]

Text
pure version_Kanaras_manuscript_2D & UCNPs - Accepted Manuscript
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More information

Accepted/In Press date: 5 October 2020
e-pub ahead of print date: 10 February 2021
Keywords: DNA sensor, MoS, WS, two-dimensional materials, upconversion nanoparticles

Identifiers

Local EPrints ID: 447085
URI: http://eprints.soton.ac.uk/id/eprint/447085
ISSN: 2095-0179
PURE UUID: b59015e7-ed7c-434e-b5e9-7b6bd5c0ff8d
ORCID for Konstantina Alexaki: ORCID iD orcid.org/0000-0002-8702-2696
ORCID for Otto Muskens: ORCID iD orcid.org/0000-0003-0693-5504
ORCID for Antonios Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

Catalogue record

Date deposited: 03 Mar 2021 17:30
Last modified: 26 Nov 2021 03:12

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Contributors

Author: Davide Giust
Author: Maria-Eleni Kyriazi
Author: Afaf H. El-Sagheer
Author: Tom Brown
Author: Otto Muskens ORCID iD

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