Engineered honey: In vitro antimicrobial activity of a novel topical wound care treatment
Engineered honey: In vitro antimicrobial activity of a novel topical wound care treatment
Surgihoney is a novel engineered organic honey product for wound care. Its antimicrobial activity can be controlled and adjusted by the engineering process, allowing preparation of three different potencies, labelled Surgihoney 1-3. Susceptibility testing of a range of wound and ulcer bacterial isolates to Surgihoney by the disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination, and time-kill measurements by time suspension tests were performed. Surgihoney demonstrated highly potent inhibitory and cidal activity against a wide range of Gram-positive and Gram-negative bacteria and fungi. MICs/MBCs were significantly lower than concentrations likely to be achieved in topical clinical use. The topical concentration of Surgihoney in wounds was estimated at ca. 500 g/L. MICs/MBCs for Staphylococcus aureus were 32/125 g/L for Surgihoney 1 and 0.12/0.25 g/L for Surgihoney 3. Cidal speed depended on potency, being 48 h for Surgihoney 1 and 30 min for Surgihoney 3. Maintenance of the Surgihoney inoculum preparation for up to a week demonstrated complete cidal activity and no bacterial persistence. Surgihoney has wide potential as a highly active topical treatment combining the effects of the healing properties of honey with the potent antimicrobial activity of the engineered product for skin lesions, wounds, ulcers and cavities. It is highly active against multidrug-resistant bacteria. It is more active than other honeys tested and is comparable with chemical antiseptics in antimicrobial activity.
Honey, MBC, MIC, Soft tissue infection, Surgihoney, Tissue viability, Topical therapy
168-172
Dryden, Matthew
a6c300f9-5c26-4884-980b-c098b0688ab1
Lockyer, Gemma
6d2610e3-c3eb-46c5-8625-e237b706fdf8
Saeed, Kordo
87cb67e5-71e8-4759-bf23-2ea00ebd8b39
Cooke, Jonathan
66a01b74-cc14-4831-a7cb-231e692e8a70
September 2014
Dryden, Matthew
a6c300f9-5c26-4884-980b-c098b0688ab1
Lockyer, Gemma
6d2610e3-c3eb-46c5-8625-e237b706fdf8
Saeed, Kordo
87cb67e5-71e8-4759-bf23-2ea00ebd8b39
Cooke, Jonathan
66a01b74-cc14-4831-a7cb-231e692e8a70
Dryden, Matthew, Lockyer, Gemma, Saeed, Kordo and Cooke, Jonathan
(2014)
Engineered honey: In vitro antimicrobial activity of a novel topical wound care treatment.
Journal of Global Antimicrobial Resistance, 2 (3), .
(doi:10.1016/j.jgar.2014.03.006).
Abstract
Surgihoney is a novel engineered organic honey product for wound care. Its antimicrobial activity can be controlled and adjusted by the engineering process, allowing preparation of three different potencies, labelled Surgihoney 1-3. Susceptibility testing of a range of wound and ulcer bacterial isolates to Surgihoney by the disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination, and time-kill measurements by time suspension tests were performed. Surgihoney demonstrated highly potent inhibitory and cidal activity against a wide range of Gram-positive and Gram-negative bacteria and fungi. MICs/MBCs were significantly lower than concentrations likely to be achieved in topical clinical use. The topical concentration of Surgihoney in wounds was estimated at ca. 500 g/L. MICs/MBCs for Staphylococcus aureus were 32/125 g/L for Surgihoney 1 and 0.12/0.25 g/L for Surgihoney 3. Cidal speed depended on potency, being 48 h for Surgihoney 1 and 30 min for Surgihoney 3. Maintenance of the Surgihoney inoculum preparation for up to a week demonstrated complete cidal activity and no bacterial persistence. Surgihoney has wide potential as a highly active topical treatment combining the effects of the healing properties of honey with the potent antimicrobial activity of the engineered product for skin lesions, wounds, ulcers and cavities. It is highly active against multidrug-resistant bacteria. It is more active than other honeys tested and is comparable with chemical antiseptics in antimicrobial activity.
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More information
Accepted/In Press date: 26 April 2014
e-pub ahead of print date: 26 April 2014
Published date: September 2014
Additional Information:
Funding Information:
This study was supported through a research grant to Hampshire Hospitals Foundation Trust (Winchester, UK) from Healing Honey International (Bicester, UK) .
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
Keywords:
Honey, MBC, MIC, Soft tissue infection, Surgihoney, Tissue viability, Topical therapy
Identifiers
Local EPrints ID: 447336
URI: http://eprints.soton.ac.uk/id/eprint/447336
ISSN: 2213-7165
PURE UUID: 522d4f39-7793-47e3-b36c-6e4a3438426d
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Date deposited: 09 Mar 2021 17:33
Last modified: 18 Mar 2024 03:52
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Contributors
Author:
Matthew Dryden
Author:
Gemma Lockyer
Author:
Kordo Saeed
Author:
Jonathan Cooke
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