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Proteomic analysis of Neisseria lactamica and Neisseria meningitidis outer membrane vesicle vaccine antigens

Proteomic analysis of Neisseria lactamica and Neisseria meningitidis outer membrane vesicle vaccine antigens
Proteomic analysis of Neisseria lactamica and Neisseria meningitidis outer membrane vesicle vaccine antigens
Vaccines to prevent meningococcal disease have been developed from the outer membrane vesicles (OMVs) of Neisseria meningitidis and the related commensal organism Neisseria lactamica. In addition to lipopolysaccharide and the major porins, these vaccines contain a large number of proteins that are incompletely characterised. Here we describe comparative proteomic analyses of the N. lactamica OMV vaccine and OMVs from a serogroup B strain of N. meningitidis. Tandem mass-spectrometry data for trypsinised N. lactamica OMV vaccine were matched to an incompletely assembled genome sequence from the same strain to give 65 robust protein identifications and a further 122 single- or two-peptide matches. Fifty-seven N. meningitidis K454 proteins were identified robustly (and a further 68 from single- or two-peptide matches) by inference from the N. meningitidis MC58 genome. The results suggest that OMVs have a hitherto unappreciated complexity and pinpoint novel candidate antigens for further characterisation.
neisseria meningitidis, neisseria lactamica, vaccine, outer membrane vesicle, proteomics, tandem mass spectrometry
5277-5293
Vaughan, Thomas E.
e2145ba3-9c29-4c4b-8736-94274852afc4
Skipp, Paul J.
1ba7dcf6-9fe7-4b5c-a9d0-e32ed7f42aa5
O'Connor, C. David
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Hudson, Michael J.
ac017636-7fe1-4bea-99d1-86b5442a6b62
Vipond, Richard
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Elmore, Michael J.
5aeb09fd-341e-47f1-8077-eb0a6d0bbcd4
Gorringe, Andrew R.
00257a3d-0c2b-479b-ab37-c7b7f65445a3
Vaughan, Thomas E.
e2145ba3-9c29-4c4b-8736-94274852afc4
Skipp, Paul J.
1ba7dcf6-9fe7-4b5c-a9d0-e32ed7f42aa5
O'Connor, C. David
17ff63ee-30d8-44c5-84b5-775d51e45d46
Hudson, Michael J.
ac017636-7fe1-4bea-99d1-86b5442a6b62
Vipond, Richard
96592d62-38e0-4993-98b6-3085d82b49ba
Elmore, Michael J.
5aeb09fd-341e-47f1-8077-eb0a6d0bbcd4
Gorringe, Andrew R.
00257a3d-0c2b-479b-ab37-c7b7f65445a3

Vaughan, Thomas E., Skipp, Paul J., O'Connor, C. David, Hudson, Michael J., Vipond, Richard, Elmore, Michael J. and Gorringe, Andrew R. (2006) Proteomic analysis of Neisseria lactamica and Neisseria meningitidis outer membrane vesicle vaccine antigens. Vaccine, 24 (25), 5277-5293. (doi:10.1016/j.vaccine.2006.03.013).

Record type: Article

Abstract

Vaccines to prevent meningococcal disease have been developed from the outer membrane vesicles (OMVs) of Neisseria meningitidis and the related commensal organism Neisseria lactamica. In addition to lipopolysaccharide and the major porins, these vaccines contain a large number of proteins that are incompletely characterised. Here we describe comparative proteomic analyses of the N. lactamica OMV vaccine and OMVs from a serogroup B strain of N. meningitidis. Tandem mass-spectrometry data for trypsinised N. lactamica OMV vaccine were matched to an incompletely assembled genome sequence from the same strain to give 65 robust protein identifications and a further 122 single- or two-peptide matches. Fifty-seven N. meningitidis K454 proteins were identified robustly (and a further 68 from single- or two-peptide matches) by inference from the N. meningitidis MC58 genome. The results suggest that OMVs have a hitherto unappreciated complexity and pinpoint novel candidate antigens for further characterisation.

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More information

Published date: 2006
Keywords: neisseria meningitidis, neisseria lactamica, vaccine, outer membrane vesicle, proteomics, tandem mass spectrometry

Identifiers

Local EPrints ID: 44736
URI: http://eprints.soton.ac.uk/id/eprint/44736
PURE UUID: 231c2c5b-6153-4fbb-959d-35f5cf34bfe5
ORCID for Paul J. Skipp: ORCID iD orcid.org/0000-0002-2995-2959

Catalogue record

Date deposited: 09 Mar 2007
Last modified: 23 Jul 2022 01:35

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Contributors

Author: Thomas E. Vaughan
Author: Paul J. Skipp ORCID iD
Author: C. David O'Connor
Author: Michael J. Hudson
Author: Richard Vipond
Author: Michael J. Elmore
Author: Andrew R. Gorringe

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