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Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection

Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection
Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is continuing to disrupt personal lives, global healthcare systems, and economies. Hence, there is an urgent need for a vaccine that prevents viral infection, transmission, and disease. Here, we present a two-component protein-based nanoparticle vaccine that displays multiple copies of the SARS-CoV-2 spike protein. Immunization studies show that this vaccine induces potent neutralizing antibody responses in mice, rabbits, and cynomolgus macaques. The vaccine-induced immunity protects macaques against a high-dose challenge, resulting in strongly reduced viral infection and replication in the upper and lower airways. These nanoparticles are a promising vaccine candidate to curtail the SARS-CoV-2 pandemic. Brouwer et al. present preclinical evidence in support of a COVID-19 vaccine candidate, designed as a self-assembling two-component protein nanoparticle displaying multiple copies of the SARS-CoV-2 spike protein, which induces strong neutralizing antibody responses and protects from high-dose SARS-CoV-2 challenge.

B cells, COVID-19, SARS-CoV-2, antibodies, immunity, macaques, nanoparticles, protection, vaccine
0092-8674
1188-1200.e19
Brouwer, Philip J M
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Grobben, Marloes
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Crispin, Max
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King, Neil P
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van der Werf, Sylvie
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van Gils, Marit J
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Le Grand, Roger
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Sanders, Rogier W
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Brouwer, Philip J M
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Dereuddre-Bosquet, Nathalie
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Grobben, Marloes
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de Gast, Marlon
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Marlin, Romain
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Chesnais, Virginie
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Diry, Ségolène
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Allen, Joel D
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van Breemen, Marielle J
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Caniels, Tom G
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Chapon, Catherine
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Fang, Raphaël Ho Tsong
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Villaudy, Julien
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de Bree, Godelieve J
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Ginoux, Eric
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Ward, Andrew B
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Crispin, Max
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King, Neil P
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van der Werf, Sylvie
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van Gils, Marit J
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Le Grand, Roger
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Sanders, Rogier W
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Brouwer, Philip J M, Brinkkemper, Mitch, Maisonnasse, Pauline, Dereuddre-Bosquet, Nathalie, Grobben, Marloes, Claireaux, Mathieu, de Gast, Marlon, Marlin, Romain, Chesnais, Virginie, Diry, Ségolène, Allen, Joel D, Watanabe, Yasunori, Giezen, Julia M, Kerster, Gius, van der Straten, Karlijn, van der Linden, Cynthia A, Aldon, Yoann, Naninck, Thibaut, Bontjer, Ilja, Burger, Judith A, Poniman, Meliawati, Mykytyn, Anna Z, Okba, Nisreen M A, Schermer, Edith E, van Breemen, Marielle J, Ravichandran, Rashmi, Caniels, Tom G, van Schooten, Jelle, Kahlaoui, Nidhal, Contreras, Vanessa, Lemaître, Julien, Chapon, Catherine, Fang, Raphaël Ho Tsong, Villaudy, Julien, Sliepen, Kwinten, van der Velden, Yme U, Haagmans, Bart L, de Bree, Godelieve J, Ginoux, Eric, Ward, Andrew B, Crispin, Max, King, Neil P, van der Werf, Sylvie, van Gils, Marit J, Le Grand, Roger and Sanders, Rogier W (2021) Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection. Cell, 184 (5), 1188-1200.e19. (doi:10.1016/j.cell.2021.01.035).

Record type: Article

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is continuing to disrupt personal lives, global healthcare systems, and economies. Hence, there is an urgent need for a vaccine that prevents viral infection, transmission, and disease. Here, we present a two-component protein-based nanoparticle vaccine that displays multiple copies of the SARS-CoV-2 spike protein. Immunization studies show that this vaccine induces potent neutralizing antibody responses in mice, rabbits, and cynomolgus macaques. The vaccine-induced immunity protects macaques against a high-dose challenge, resulting in strongly reduced viral infection and replication in the upper and lower airways. These nanoparticles are a promising vaccine candidate to curtail the SARS-CoV-2 pandemic. Brouwer et al. present preclinical evidence in support of a COVID-19 vaccine candidate, designed as a self-assembling two-component protein nanoparticle displaying multiple copies of the SARS-CoV-2 spike protein, which induces strong neutralizing antibody responses and protects from high-dose SARS-CoV-2 challenge.

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Two-component spike nanoparticle - Version of Record
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More information

Accepted/In Press date: 21 January 2021
e-pub ahead of print date: 26 January 2021
Published date: 4 March 2021
Keywords: B cells, COVID-19, SARS-CoV-2, antibodies, immunity, macaques, nanoparticles, protection, vaccine
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Identifiers

Local EPrints ID: 447390
URI: http://eprints.soton.ac.uk/id/eprint/447390
DOI: doi:10.1016/j.cell.2021.01.035
ISSN: 0092-8674
PURE UUID: 081b1da2-0fc5-4275-940d-4045a30227c6
ORCID for Joel D Allen: ORCID iD orcid.org/0000-0003-2547-968X
ORCID for Max Crispin: ORCID iD orcid.org/0000-0002-1072-2694

Catalogue record

Date deposited: 10 Mar 2021 17:42
Last modified: 06 Jun 2024 02:11

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Contributors

Author: Philip J M Brouwer
Author: Mitch Brinkkemper
Author: Pauline Maisonnasse
Author: Nathalie Dereuddre-Bosquet
Author: Marloes Grobben
Author: Mathieu Claireaux
Author: Marlon de Gast
Author: Romain Marlin
Author: Virginie Chesnais
Author: Ségolène Diry
Author: Joel D Allen ORCID iD
Author: Yasunori Watanabe
Author: Julia M Giezen
Author: Gius Kerster
Author: Karlijn van der Straten
Author: Cynthia A van der Linden
Author: Yoann Aldon
Author: Thibaut Naninck
Author: Ilja Bontjer
Author: Judith A Burger
Author: Meliawati Poniman
Author: Anna Z Mykytyn
Author: Nisreen M A Okba
Author: Edith E Schermer
Author: Marielle J van Breemen
Author: Rashmi Ravichandran
Author: Tom G Caniels
Author: Jelle van Schooten
Author: Nidhal Kahlaoui
Author: Vanessa Contreras
Author: Julien Lemaître
Author: Catherine Chapon
Author: Raphaël Ho Tsong Fang
Author: Julien Villaudy
Author: Kwinten Sliepen
Author: Yme U van der Velden
Author: Bart L Haagmans
Author: Godelieve J de Bree
Author: Eric Ginoux
Author: Andrew B Ward
Author: Max Crispin ORCID iD
Author: Neil P King
Author: Sylvie van der Werf
Author: Marit J van Gils
Author: Roger Le Grand
Author: Rogier W Sanders

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