Immunofocusing and enhancing autologous Tier-2 HIV-1 neutralization by displaying Env trimers on two-component protein nanoparticles

e-pub ahead of print date: 9 February 2021

Published date: 9 February 2021

Additional Information: Funding Information: The authors thank Michel Nussenzweig, James Robinson, Dennis Burton, Peter Kwong, Mark Connors, John Mascola, and William Olson for donating antibodies and reagents directly or through the NIH AIDS Research and Reference Reagent Program. The following reagent was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: Ramos B cells from Drs. Li Wu and Vineet N. KewalRaman. We thank Andrew McGuire for kindly sharing the pRRL.EuB29 lentiviral vector that was used to transduce Ramos B cells. This work was supported by the U. S. National Institutes of Health Grant P01 AI110657 (to J.P.M., A.B.W., P.J.K., and R.W. S.) and NIAID Contract #HHSN27201100016C (to D.C.M.); by the Bill and Melinda Gates Foundation through the Collaboration for AIDS Vaccine Discovery (CAVD), grants OPP1111923 and OPP1132237 (to N.K., J.P.M., and R.W.S.), INV-008352/ OPP1153692 (to M.C.), and OPP1115782 (to A.B.W.); by the National Institute for Allergy and Infectious Diseases through the Scripps Consortium for HIV Vaccine Development (CHAVD) grant AI144462 (to M.C.); by the European Union’s Horizon 2020 research and innovation program under grant agreement No. 681137 (to R.W. S. and M.C.); and by the Fondation Dormeur, Vaduz (to R.W.S.). R.W.S. is a recipient of a Vici grant from the Netherlands Organization for Scientific Research (NWO). M.J.G. is a recipient of an AMC Fellowship and a Mathilde Krim Fellowship from the American Foundation for AIDS Research (amfAR) (109514-61-RKVA). The electron microscopy data were collected at Electron Microscopy Facility of the Scripps Research Institute. Publisher Copyright: © 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

Local EPrints ID: 447559

URI: http://eprints.soton.ac.uk/id/eprint/447559

DOI: doi:10.1038/s41541-021-00285-9

ISSN: 2059-0105

PURE UUID: aeb00058-0683-4408-82be-1f53b47e0706

ORCID for Joel D Allen: orcid.org/0000-0003-2547-968X

ORCID for Max Crispin: orcid.org/0000-0002-1072-2694

Date deposited: 16 Mar 2021 17:31

Last modified: 12 Jul 2024 02:09