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Epigenome-scale comparison of DNA methylation between blood leukocytes and bronchial epithelial cells

Epigenome-scale comparison of DNA methylation between blood leukocytes and bronchial epithelial cells
Epigenome-scale comparison of DNA methylation between blood leukocytes and bronchial epithelial cells
Aim: agreement in DNA methylation (DNAm) at the genome scale between blood leukocytes (BL) and bronchial epithelial cells (BEC) is unknown. We examine as to what extent DNAm in BL is comparable with that in BEC and serves as a surrogate for BEC. Methods: overall agreement (paired t-tests with false discovery rate adjusted p-value >0.05) and consistency (Pearson’s correlation coefficients >0.5) between two tissues, at each of the 767,412 CpGs, were evaluated. Results & Conclusion: we identified 247,721 CpGs showing overall agreement and 47,371 CpGs showing consistency in DNAm. Identified CpGs are involved in certain immune pathways, indicating the potential of using blood as a biomarker for BEC at those CpGs in lower airway related diseases. CpGs showing overall agreement and those without overall agreement are distributed differently on the genome.
DNA methylation, Isle of Wight, blood leukocytes, bronchial epithelial cells
1750-1911
485-498
Lee, Yu-Sheng
8f8522b4-f7f2-4e2b-b20a-af67ceb42c7b
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Jiang, Yu
4fa0a27b-8c06-4988-832a-8f5fb3a3f1ec
Perunthadambil Kadalayil, Latha
e620b801-844a-45d9-acaf-e0a58acd7cf2
Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853
Ewart, Susan L.
48158071-d7b8-46b5-8aef-26ffb37eec3b
Arshad, Syed
917e246d-2e60-472f-8d30-94b01ef28958
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Lee, Yu-Sheng
8f8522b4-f7f2-4e2b-b20a-af67ceb42c7b
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Jiang, Yu
4fa0a27b-8c06-4988-832a-8f5fb3a3f1ec
Perunthadambil Kadalayil, Latha
e620b801-844a-45d9-acaf-e0a58acd7cf2
Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853
Ewart, Susan L.
48158071-d7b8-46b5-8aef-26ffb37eec3b
Arshad, Syed
917e246d-2e60-472f-8d30-94b01ef28958
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a

Lee, Yu-Sheng, Zhang, Hongmei, Jiang, Yu, Perunthadambil Kadalayil, Latha, Karmaus, Wilfried, Ewart, Susan L., Arshad, Syed and Holloway, John (2021) Epigenome-scale comparison of DNA methylation between blood leukocytes and bronchial epithelial cells. Epigenomics, 13 (7), 485-498. (doi:10.2217/epi-2020-0384).

Record type: Article

Abstract

Aim: agreement in DNA methylation (DNAm) at the genome scale between blood leukocytes (BL) and bronchial epithelial cells (BEC) is unknown. We examine as to what extent DNAm in BL is comparable with that in BEC and serves as a surrogate for BEC. Methods: overall agreement (paired t-tests with false discovery rate adjusted p-value >0.05) and consistency (Pearson’s correlation coefficients >0.5) between two tissues, at each of the 767,412 CpGs, were evaluated. Results & Conclusion: we identified 247,721 CpGs showing overall agreement and 47,371 CpGs showing consistency in DNAm. Identified CpGs are involved in certain immune pathways, indicating the potential of using blood as a biomarker for BEC at those CpGs in lower airway related diseases. CpGs showing overall agreement and those without overall agreement are distributed differently on the genome.

Text
Lee et al 2021 Epigenomics Blood BEC comparison AAM - Accepted Manuscript
Restricted to Repository staff only until 23 February 2022.
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More information

Accepted/In Press date: 23 February 2021
e-pub ahead of print date: 19 March 2021
Keywords: DNA methylation, Isle of Wight, blood leukocytes, bronchial epithelial cells

Identifiers

Local EPrints ID: 447591
URI: http://eprints.soton.ac.uk/id/eprint/447591
ISSN: 1750-1911
PURE UUID: 3c6370c7-5519-43a7-a38c-cff2823fb4d7
ORCID for John Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 16 Mar 2021 17:45
Last modified: 26 Nov 2021 02:40

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Contributors

Author: Yu-Sheng Lee
Author: Hongmei Zhang
Author: Yu Jiang
Author: Wilfried Karmaus
Author: Susan L. Ewart
Author: Syed Arshad
Author: John Holloway ORCID iD

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