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Protein signatures that promote operator selectivity among Paralog MerR monovalent metal ion regulators

Protein signatures that promote operator selectivity among Paralog MerR monovalent metal ion regulators
Protein signatures that promote operator selectivity among Paralog MerR monovalent metal ion regulators
Two paralog transcriptional regulators of the MerR family, CueR and GolS, are responsible for monovalent metal ion sensing and resistance in Salmonella enterica. Although similar in sequence and also in their target binding sites, these proteins differ in signal detection and in the set of target genes they control. Recently, we demonstrated that selective promoter recognition depends on the presence of specific bases located at positions 3' and 3 within the operators they interact with. Here, we identify the amino acid residues within the N-terminal DNA-binding domain of these sensor proteins that are directly involved in operator discrimination. We demonstrate that a methionine residue at position 16 of GolS, absolutely conserved among GolS-like proteins but absent in all CueR-like xenologs, is the key to selectively recognize operators that harbor the distinctive GolS-operator signature, whereas the residue at position 19 finely tunes the regulator/operator interaction. Furthermore, swapping these residues switches the set of genes recognized by these transcription factors. These results indicate that co-evolution of a regulator and its cognate operators within the bacterial cell provides the conditions to avoid cross-recognition and guarantees the proper response to metal injury.
0021-9258
Humbert, Maria
82134d25-24b8-4fdd-bd1c-461683b5322e
Rasia, Rodolfo M
6716f13e-779f-4fcb-8f18-c1bfd2821116
Checa, Susana K
a8da79bd-7058-43ae-8c63-6a3ee8e3fdd9
Soncini, Fernando C
e2e4ddb8-a178-4b6c-9249-0f4be8b8ccda
Humbert, Maria
82134d25-24b8-4fdd-bd1c-461683b5322e
Rasia, Rodolfo M
6716f13e-779f-4fcb-8f18-c1bfd2821116
Checa, Susana K
a8da79bd-7058-43ae-8c63-6a3ee8e3fdd9
Soncini, Fernando C
e2e4ddb8-a178-4b6c-9249-0f4be8b8ccda

Humbert, Maria, Rasia, Rodolfo M, Checa, Susana K and Soncini, Fernando C (2013) Protein signatures that promote operator selectivity among Paralog MerR monovalent metal ion regulators. The Journal of Biological Chemistry, 288 (28). (doi:10.1074/jbc.M113.452797).

Record type: Article

Abstract

Two paralog transcriptional regulators of the MerR family, CueR and GolS, are responsible for monovalent metal ion sensing and resistance in Salmonella enterica. Although similar in sequence and also in their target binding sites, these proteins differ in signal detection and in the set of target genes they control. Recently, we demonstrated that selective promoter recognition depends on the presence of specific bases located at positions 3' and 3 within the operators they interact with. Here, we identify the amino acid residues within the N-terminal DNA-binding domain of these sensor proteins that are directly involved in operator discrimination. We demonstrate that a methionine residue at position 16 of GolS, absolutely conserved among GolS-like proteins but absent in all CueR-like xenologs, is the key to selectively recognize operators that harbor the distinctive GolS-operator signature, whereas the residue at position 19 finely tunes the regulator/operator interaction. Furthermore, swapping these residues switches the set of genes recognized by these transcription factors. These results indicate that co-evolution of a regulator and its cognate operators within the bacterial cell provides the conditions to avoid cross-recognition and guarantees the proper response to metal injury.

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More information

Accepted/In Press date: 3 June 2013
Published date: 12 July 2013

Identifiers

Local EPrints ID: 447599
URI: http://eprints.soton.ac.uk/id/eprint/447599
ISSN: 0021-9258
PURE UUID: 6a72cb91-5fec-4c2d-be41-06d7a1445d12
ORCID for Maria Humbert: ORCID iD orcid.org/0000-0002-5728-6981

Catalogue record

Date deposited: 16 Mar 2021 17:46
Last modified: 07 Oct 2021 01:46

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Contributors

Author: Maria Humbert ORCID iD
Author: Rodolfo M Rasia
Author: Susana K Checa
Author: Fernando C Soncini

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