Near-clinical applications of laser scanning cytometry
Near-clinical applications of laser scanning cytometry
Biological samples from human tissues are characterized by complexity and heterogeneity. The ability to make rapid, reliable, quantitative fluorochromatic measurements on clinical samples allows the development of new and practical assays that could influence diagnosis and treatment in a variety of clinical applications. Laser scanning cytometry (LSC) is a very versatile and adaptable technology that allows for the quantitative analysis of cell samples that are unsuitable for flow cytometry by virtue of their presentation and context. Crucially, it allows the direct visualization of cells and rare events and the correlation of imagery with fluorochromatic measurements. In this chapter, we describe early experiments in the study of cytotoxic drug uptake and resistance in human tumor cells and in the study of sputum cells from asthmatic patients, which harness the specific capabilities of LSC to practical clinical problems.
193-212
Rew, David A.
36dcc3ad-2379-4b61-a468-5c623d796887
Woltmann, Gerrit
51d70e50-a7a2-4f32-8708-6c3c8f8f5a5b
Kaur, Davinder
6d579e63-1800-495d-91e5-8ba38b0880f6
2006
Rew, David A.
36dcc3ad-2379-4b61-a468-5c623d796887
Woltmann, Gerrit
51d70e50-a7a2-4f32-8708-6c3c8f8f5a5b
Kaur, Davinder
6d579e63-1800-495d-91e5-8ba38b0880f6
Rew, David A., Woltmann, Gerrit and Kaur, Davinder
(2006)
Near-clinical applications of laser scanning cytometry.
In,
Taatjes, Douglas J. and Mossman, Brooke T.
(eds.)
Cell Imaging Techniques: Methods and Protocols.
(Methods in Molecular Biology, 319)
Humana Press, .
(doi:10.1007/978-1-59259-993-6_9).
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Book Section
Abstract
Biological samples from human tissues are characterized by complexity and heterogeneity. The ability to make rapid, reliable, quantitative fluorochromatic measurements on clinical samples allows the development of new and practical assays that could influence diagnosis and treatment in a variety of clinical applications. Laser scanning cytometry (LSC) is a very versatile and adaptable technology that allows for the quantitative analysis of cell samples that are unsuitable for flow cytometry by virtue of their presentation and context. Crucially, it allows the direct visualization of cells and rare events and the correlation of imagery with fluorochromatic measurements. In this chapter, we describe early experiments in the study of cytotoxic drug uptake and resistance in human tumor cells and in the study of sputum cells from asthmatic patients, which harness the specific capabilities of LSC to practical clinical problems.
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Published date: 2006
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Identifiers
Local EPrints ID: 447629
URI: http://eprints.soton.ac.uk/id/eprint/447629
ISSN: 1064-3745
PURE UUID: b091b7b2-0dd4-4931-b175-6b4011925ce0
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Date deposited: 17 Mar 2021 17:32
Last modified: 17 Mar 2024 03:56
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Contributors
Author:
Gerrit Woltmann
Author:
Davinder Kaur
Editor:
Douglas J. Taatjes
Editor:
Brooke T. Mossman
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