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Comparison between turnaround time and cost of herpes simplex virus testing by cell culture and polymerase chain reaction from genital swabs

Comparison between turnaround time and cost of herpes simplex virus testing by cell culture and polymerase chain reaction from genital swabs
Comparison between turnaround time and cost of herpes simplex virus testing by cell culture and polymerase chain reaction from genital swabs

Clinical diagnosis of genital herpes simplex virus (HSV) (GH) infection is insensitive and non-specific and requires laboratory confirmation. In this study we compared viral culture and the amplification of HSV DNA by polymerase chain reaction (PCR) with respect to turnaround time and cost. We compared 182 swabs submitted to our laboratory between March and May 2005, which were tested using MRC-5 cell culture, with 168 genital swabs submitted during the same months in 2006, and were tested by PCR. We concluded that PCR testing in our laboratory has significantly improved the turnaround time, with 68.4% of tests having been reported in less than 24 hours. This in turn significantly improved the service provided for the diagnosis of genital herpes without additional costs.

Diagnosis, Genital, Herpes simplex virus, PCR
0956-4624
298-299
Saeed, K.
87cb67e5-71e8-4759-bf23-2ea00ebd8b39
Pelosi, E.
b931aafb-7801-460f-915a-244ac1ef79b5
Saeed, K.
87cb67e5-71e8-4759-bf23-2ea00ebd8b39
Pelosi, E.
b931aafb-7801-460f-915a-244ac1ef79b5

Saeed, K. and Pelosi, E. (2010) Comparison between turnaround time and cost of herpes simplex virus testing by cell culture and polymerase chain reaction from genital swabs. International Journal of STD and AIDS, 21 (4), 298-299. (doi:10.1258/ijsa.2009.009006).

Record type: Article

Abstract

Clinical diagnosis of genital herpes simplex virus (HSV) (GH) infection is insensitive and non-specific and requires laboratory confirmation. In this study we compared viral culture and the amplification of HSV DNA by polymerase chain reaction (PCR) with respect to turnaround time and cost. We compared 182 swabs submitted to our laboratory between March and May 2005, which were tested using MRC-5 cell culture, with 168 genital swabs submitted during the same months in 2006, and were tested by PCR. We concluded that PCR testing in our laboratory has significantly improved the turnaround time, with 68.4% of tests having been reported in less than 24 hours. This in turn significantly improved the service provided for the diagnosis of genital herpes without additional costs.

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More information

Published date: April 2010
Additional Information: Copyright: Copyright 2010 Elsevier B.V., All rights reserved.
Keywords: Diagnosis, Genital, Herpes simplex virus, PCR

Identifiers

Local EPrints ID: 447708
URI: http://eprints.soton.ac.uk/id/eprint/447708
ISSN: 0956-4624
PURE UUID: 61e70e1b-df4b-4cf3-9429-192fcbe9e586
ORCID for K. Saeed: ORCID iD orcid.org/0000-0003-0123-0302

Catalogue record

Date deposited: 18 Mar 2021 17:52
Last modified: 17 Mar 2024 03:56

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Contributors

Author: K. Saeed ORCID iD
Author: E. Pelosi

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