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The fatty acid composition of human follicular fluid is altered by a six-week dietary intervention that includes marine omega-3 fatty acids

The fatty acid composition of human follicular fluid is altered by a six-week dietary intervention that includes marine omega-3 fatty acids
The fatty acid composition of human follicular fluid is altered by a six-week dietary intervention that includes marine omega-3 fatty acids
The fatty acid composition of human follicular fluid is important for oocyte development and for pregnancy following in vitro fertilization (IVF). This study investigated whether a dietary intervention that included an increase in marine omega‐3 fatty acids, olive oil and vitamin D alters the fatty acid composition of human follicular fluid. The association of lifestyle factors with follicular fluid fatty acid composition was also investigated. Fifty‐five couples awaiting IVF were randomized to receive the 6‐week treatment intervention of olive oil for cooking, an olive oil‐based spread, and a daily supplement drink enriched with vitamin D and the marine omega‐3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) and 56 couples were randomized to receive placebo equivalents. Dietary questionnaires were completed, and samples of blood were taken before and after the intervention. Follicular fluid was collected at oocyte retrieval and the fatty acid profile assessed using gas chromatography. In the control group, individual fatty acids in red blood cells and follicular fluid were significantly correlated. Furthermore, a healthier diet was associated with a lower percentage of follicular fluid arachidonic acid. The follicular fluid of women in the treatment group contained significantly higher amounts of EPA and DHA compared to the control group, while the omega‐6 fatty acids linoleic, γ‐linolenic, dihomo‐γ‐linolenic, and arachidonic were lower. This is the first report of a dietary intervention altering the fatty acid composition of follicular fluid in humans. Further research is required to determine whether this intervention improves oocyte quality.

0024-4201
201-209
Kermack, Alexandra Jayne
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Wellstead, Susan J
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Fisk, Helena Lucy
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Cheong, Ying
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Houghton, Franchesca D
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Macklon, Nicholas
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e
Calder, Philip
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Kermack, Alexandra Jayne
b01db302-47bf-4319-a270-4cbbf26045c8
Wellstead, Susan J
52205020-a7bf-4622-8b5d-cd83fac3767b
Fisk, Helena Lucy
38c7f1f0-5dfc-4f71-aa0f-ec4f0e5839f3
Cheong, Ying
4efbba2a-3036-4dce-82f1-8b4017952c83
Houghton, Franchesca D
53946041-127e-45a8-9edb-bf4b3c23005f
Macklon, Nicholas
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e
Calder, Philip
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Kermack, Alexandra Jayne, Wellstead, Susan J, Fisk, Helena Lucy, Cheong, Ying, Houghton, Franchesca D, Macklon, Nicholas and Calder, Philip (2021) The fatty acid composition of human follicular fluid is altered by a six-week dietary intervention that includes marine omega-3 fatty acids. Lipids, 56 (2), 201-209. (doi:10.1002/lipd.12288).

Record type: Article

Abstract

The fatty acid composition of human follicular fluid is important for oocyte development and for pregnancy following in vitro fertilization (IVF). This study investigated whether a dietary intervention that included an increase in marine omega‐3 fatty acids, olive oil and vitamin D alters the fatty acid composition of human follicular fluid. The association of lifestyle factors with follicular fluid fatty acid composition was also investigated. Fifty‐five couples awaiting IVF were randomized to receive the 6‐week treatment intervention of olive oil for cooking, an olive oil‐based spread, and a daily supplement drink enriched with vitamin D and the marine omega‐3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) and 56 couples were randomized to receive placebo equivalents. Dietary questionnaires were completed, and samples of blood were taken before and after the intervention. Follicular fluid was collected at oocyte retrieval and the fatty acid profile assessed using gas chromatography. In the control group, individual fatty acids in red blood cells and follicular fluid were significantly correlated. Furthermore, a healthier diet was associated with a lower percentage of follicular fluid arachidonic acid. The follicular fluid of women in the treatment group contained significantly higher amounts of EPA and DHA compared to the control group, while the omega‐6 fatty acids linoleic, γ‐linolenic, dihomo‐γ‐linolenic, and arachidonic were lower. This is the first report of a dietary intervention altering the fatty acid composition of follicular fluid in humans. Further research is required to determine whether this intervention improves oocyte quality.

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Follicular fluid and diet_revision - Accepted Manuscript
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Accepted/In Press date: 22 September 2020
e-pub ahead of print date: 12 October 2020
Published date: March 2021

Identifiers

Local EPrints ID: 447715
URI: http://eprints.soton.ac.uk/id/eprint/447715
ISSN: 0024-4201
PURE UUID: 8b5f6381-8c1e-43f4-a31c-4e8a50fdc9ab
ORCID for Alexandra Jayne Kermack: ORCID iD orcid.org/0000-0002-9176-9426
ORCID for Ying Cheong: ORCID iD orcid.org/0000-0001-7687-4597
ORCID for Franchesca D Houghton: ORCID iD orcid.org/0000-0002-5167-1694
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 18 Mar 2021 17:53
Last modified: 12 Oct 2021 04:01

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Contributors

Author: Alexandra Jayne Kermack ORCID iD
Author: Susan J Wellstead
Author: Helena Lucy Fisk
Author: Ying Cheong ORCID iD
Author: Nicholas Macklon
Author: Philip Calder ORCID iD

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