The University of Southampton
University of Southampton Institutional Repository

15N hyperpolarisation of the antiprotozoal drug ornidazole by Signal Amplification By Reversible Exchange in aqueous medium

15N hyperpolarisation of the antiprotozoal drug ornidazole by Signal Amplification By Reversible Exchange in aqueous medium
15N hyperpolarisation of the antiprotozoal drug ornidazole by Signal Amplification By Reversible Exchange in aqueous medium
Signal amplification by reversible exchange (SABRE) offers a cost‐effective route to boost nuclear magnetic resonance (NMR) signal by several orders of magnitude by employing readily available para‐hydrogen as a source of hyperpolarisation. Although 1H spins have been the natural choice of SABRE hyperpolarisation since its inception due to its simplicity and accessibility, limited spin lifetimes of 1H makes it harder to employ them in a range of time‐dependent NMR experiments. Heteronuclear spins, for example, 13C and 15N, in general have much longer T1 lifetimes and thereby are found to be more suitable for hyperpolarised biological applications as demonstrated previously by para‐hydrogen induced polarisation (PHIP) and dynamic nuclear polarisation (DNP). In this study we demonstrate a simple procedure to enhance 15N signal of an antibiotic drug ornidazole by up to 71,000‐folds with net 15N polarisation reaching ~23%. Further, the effect of co‐ligand strategy is studied in conjunction with the optimum field transfer protocols and consequently achieving 15N hyperpolarised spin lifetime of >3 min at low field. Finally, we present a convenient route to harness the hyperpolarised solution in aqueous medium free from catalyst contamination leading to a strong 15N signal detection for an extended duration of time.
0749-1581
1199-1207
Iali, Wissam
c5a0dd1d-92bd-4cc2-8ae6-dad6706acf20
Moustafa, Gamal
1a452cdc-4856-4243-9864-b391fb115f07
Dagys, Laurynas
0de61597-b152-4bee-a934-123a9d2de883
Singha Roy, Soumya
fa8b7e87-8477-417a-932e-61e36581c720
Iali, Wissam
c5a0dd1d-92bd-4cc2-8ae6-dad6706acf20
Moustafa, Gamal
1a452cdc-4856-4243-9864-b391fb115f07
Dagys, Laurynas
0de61597-b152-4bee-a934-123a9d2de883
Singha Roy, Soumya
fa8b7e87-8477-417a-932e-61e36581c720

Iali, Wissam, Moustafa, Gamal, Dagys, Laurynas and Singha Roy, Soumya (2021) 15N hyperpolarisation of the antiprotozoal drug ornidazole by Signal Amplification By Reversible Exchange in aqueous medium. Magnetic Resonance in Chemistry, 59 (12), 1199-1207. (doi:10.1002/mrc.5144).

Record type: Article

Abstract

Signal amplification by reversible exchange (SABRE) offers a cost‐effective route to boost nuclear magnetic resonance (NMR) signal by several orders of magnitude by employing readily available para‐hydrogen as a source of hyperpolarisation. Although 1H spins have been the natural choice of SABRE hyperpolarisation since its inception due to its simplicity and accessibility, limited spin lifetimes of 1H makes it harder to employ them in a range of time‐dependent NMR experiments. Heteronuclear spins, for example, 13C and 15N, in general have much longer T1 lifetimes and thereby are found to be more suitable for hyperpolarised biological applications as demonstrated previously by para‐hydrogen induced polarisation (PHIP) and dynamic nuclear polarisation (DNP). In this study we demonstrate a simple procedure to enhance 15N signal of an antibiotic drug ornidazole by up to 71,000‐folds with net 15N polarisation reaching ~23%. Further, the effect of co‐ligand strategy is studied in conjunction with the optimum field transfer protocols and consequently achieving 15N hyperpolarised spin lifetime of >3 min at low field. Finally, we present a convenient route to harness the hyperpolarised solution in aqueous medium free from catalyst contamination leading to a strong 15N signal detection for an extended duration of time.

Text
15 - Version of Record
Available under License Creative Commons Attribution.
Download (1MB)

More information

Accepted/In Press date: 24 February 2021
Published date: 3 March 2021
Additional Information: © 2021 The Authors. Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd.

Identifiers

Local EPrints ID: 447760
URI: http://eprints.soton.ac.uk/id/eprint/447760
ISSN: 0749-1581
PURE UUID: 703e4d36-014e-4a2e-b236-065d659005e9
ORCID for Gamal Moustafa: ORCID iD orcid.org/0000-0002-9940-0033

Catalogue record

Date deposited: 19 Mar 2021 17:33
Last modified: 06 Jun 2024 02:05

Export record

Altmetrics

Contributors

Author: Wissam Iali
Author: Gamal Moustafa ORCID iD
Author: Laurynas Dagys
Author: Soumya Singha Roy

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×