READ ME File For 'Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids'

Dataset DOI: https://doi.org/10.5258/SOTON/D1440

ReadMe Author: Maria-Eleni Kyriazi, University of Southampton

This dataset supports the publication: Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids 
AUTHORS: Miguel Xavier, Maria-Eleni Kyriazi, Stuart A. Lanham, Konstantina Alexaki, Elloise Matthews, Afaf H. El-Sagheer, Tom Brown, Antonios G. Kanaras and Richard O.C. Oreffo
TITLE:Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids
JOURNAL: ACS Nano
PAPER DOI IF KNOWN: 10.1021/acsnano.0c10683


This dataset contains: Experimental data recorded to generate the following figures included in the publication 

The figures are as follows:

Fig. 1: FACS data for the detection of runx2 and hspa8 mRNA including a scrambled control in human BMSCs at 1, 2 and 3 hours post incubation.  

Fig. 2A: CFU – F count for sorted cells as a percentage of the CFU – F number from unselected cells

Fig. S1: Size analysis of spherical gold nanoparticles for the construction of the relevant histogram 

Fig. S2: Normalized UV-Vis spectra of gold nanoparticles and spherical nucleic acids   

Fig. S3: Zeta-potential graph showing net charge of gold nanoparticles and spherical nucleic acids

Table S2: Calculations for sense and flare strand loading

Fig. S4: Flow cytometry selection criteria for the different cell fraction regions found in human bone marrow 

Fig. S5: FACS Cy5 vs FAM dot plots shown for human bone marrow samples incubated with runx2, hspa8 and scrambled spherical nucleic acids




Date of data collection: 2018 - 2020

Information about geographic location of data collection: University of Southampton 

Licence:CC-BY

Related projects: BBSRC (BB/P017711/1)
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Date that the file was created: Feb, 2021