Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans
Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans
Infection with Aphanomyces invadans is a serious fish disease with major global impacts. Despite affecting over 160 fish species, some of the species like the common carp Cyprinus carpio are resistant to A. invadans infection. In the present study, we investigated the transcriptomes of head kidney of common carp experimentally infected with A. invadans. In time course analysis, 5288 genes were found to be differentially expressed (DEGs), of which 731 were involved in 21 immune pathways. The analysis of immune-related DEGs suggested that efficient processing and presentation of A. invadans antigens, enhanced phagocytosis, recognition of pathogen-associated molecular patterns, and increased recruitment of leukocytes to the sites of infection contribute to resistance of common carp against A. invadans. Herein, we provide a systematic understanding of the disease resistance mechanisms in common carp at molecular level as a valuable resource for developing disease management strategies for this devastating fish-pathogenic oomycete.
Aphanomyces invadans, Common carp, Disease resistance, Head kidney, Immune response, RNA sequencing
944-956
Verma, Dev Kumar
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Peruzza, Luca
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Trusch, Franziska
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Yadav, Manoj Kumar
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Ravindra,
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Shubin, Sergei V.
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Morgan, Kenton L.
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Mohindra, Vindhya
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Hauton, Chris
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Van West, Pieter
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Pradhan, P.K.
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Sood, Neeraj
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January 2021
Verma, Dev Kumar
adf3f854-bd16-4153-a355-0d5e8af9e922
Peruzza, Luca
97dfdd24-f9e8-4d76-aee5-67bdd9b92541
Trusch, Franziska
0ad6ffdf-be32-4f8b-9373-269da8b718fb
Yadav, Manoj Kumar
1779f0e5-e788-4065-9f51-c7a28d85f59d
Ravindra,
e88f54dd-01f2-4f76-a7a3-b0e6445f2d76
Shubin, Sergei V.
39a9db5b-5f62-4f3a-9836-1df6c902d46a
Morgan, Kenton L.
faba11e1-eb8a-4b45-b1e2-60b046b079ba
Mohindra, Vindhya
f07f2cc4-7038-476e-95b0-0938a266e87f
Hauton, Chris
7706f6ba-4497-42b2-8c6d-00df81676331
Van West, Pieter
9c4ff6c8-18dc-4384-9661-30b70cc7b437
Pradhan, P.K.
1764bc7f-8df4-41f2-a194-8022847b33c3
Sood, Neeraj
7fdb4eb0-5d24-4954-9073-bf98f8fbee57
Verma, Dev Kumar, Peruzza, Luca, Trusch, Franziska, Yadav, Manoj Kumar, Ravindra, , Shubin, Sergei V., Morgan, Kenton L., Mohindra, Vindhya, Hauton, Chris, Van West, Pieter, Pradhan, P.K. and Sood, Neeraj
(2021)
Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans.
Genomics, 113 (Issue 1, Part 2), .
(doi:10.1016/j.ygeno.2020.10.028).
Abstract
Infection with Aphanomyces invadans is a serious fish disease with major global impacts. Despite affecting over 160 fish species, some of the species like the common carp Cyprinus carpio are resistant to A. invadans infection. In the present study, we investigated the transcriptomes of head kidney of common carp experimentally infected with A. invadans. In time course analysis, 5288 genes were found to be differentially expressed (DEGs), of which 731 were involved in 21 immune pathways. The analysis of immune-related DEGs suggested that efficient processing and presentation of A. invadans antigens, enhanced phagocytosis, recognition of pathogen-associated molecular patterns, and increased recruitment of leukocytes to the sites of infection contribute to resistance of common carp against A. invadans. Herein, we provide a systematic understanding of the disease resistance mechanisms in common carp at molecular level as a valuable resource for developing disease management strategies for this devastating fish-pathogenic oomycete.
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More information
Accepted/In Press date: 25 October 2020
e-pub ahead of print date: 28 October 2020
Published date: January 2021
Additional Information:
Funding Information:
The work was carried out with financial support from Department of Biotechnology , Ministry of Science and Technology , Government of India (Grant number BT/IN/Indo-UK/BBSRC-qua/38/MSS/2015-16 ), and UK BBSRC , UK ESRC and UK Aid under contract BB/N005058/1 .
Funding Information:
The authors would like to express their sincere thanks to Director, ICAR-NBFGR for providing facilities to carry out this study. The work was carried out with financial support from Department of Biotechnology , Ministry of Science and Technology , Government of India (Grant number BT/IN/Indo-UK/BBSRC-qua/38/MSS/2015-16 ), and UK BBSRC , UK ESRC and UK Aid under contract BB/N005058/1 . The authors would also like to express their gratitude to Dr. Kuldeep K. Lal, Director, ICAR-NBFGR and Dr. J.K. Jena, DDG (Fy. Sc.), ICAR for their support, guidance and encouragement.
Funding Information:
The work was carried out with financial support from Department of Biotechnology, Ministry of Science and Technology, Government of India (Grant number BT/IN/Indo-UK/BBSRC-qua/38/MSS/2015-16), and UK BBSRC, UK ESRC and UK Aid under contract BB/N005058/1.The authors would like to express their sincere thanks to Director, ICAR-NBFGR for providing facilities to carry out this study. The work was carried out with financial support from Department of Biotechnology, Ministry of Science and Technology, Government of India (Grant number BT/IN/Indo-UK/BBSRC-qua/38/MSS/2015-16), and UK BBSRC, UK ESRC and UK Aid under contract BB/N005058/1. The authors would also like to express their gratitude to Dr. Kuldeep K. Lal, Director, ICAR-NBFGR and Dr. J.K. Jena, DDG (Fy. Sc.), ICAR for their support, guidance and encouragement.
Publisher Copyright:
© 2020 Elsevier Inc.
Keywords:
Aphanomyces invadans, Common carp, Disease resistance, Head kidney, Immune response, RNA sequencing
Identifiers
Local EPrints ID: 447948
URI: http://eprints.soton.ac.uk/id/eprint/447948
ISSN: 0888-7543
PURE UUID: fef30a43-ccb1-4c4f-945d-25966fade84f
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Date deposited: 26 Mar 2021 17:32
Last modified: 17 Mar 2024 02:43
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Contributors
Author:
Dev Kumar Verma
Author:
Luca Peruzza
Author:
Franziska Trusch
Author:
Manoj Kumar Yadav
Author:
Ravindra
Author:
Sergei V. Shubin
Author:
Kenton L. Morgan
Author:
Vindhya Mohindra
Author:
Pieter Van West
Author:
P.K. Pradhan
Author:
Neeraj Sood
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