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Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids

Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids
Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids
Human bone marrow (BM)-derived stromal cells contain a population of skeletal stem cells (SSCs), with the capacity to differentiate along the osteogenic, adipogenic, and chondrogenic lineages, enabling their application to clinical therapies. However, current methods to isolate and enrich SSCs from human tissues remain, at best, challenging in the absence of a specific SSC marker. Unfortunately, none of the current proposed markers alone can isolate a homogeneous cell population with the ability to form bone, cartilage, and adipose tissue in humans. Here, we have designed DNA-gold nanoparticles able to identify and sort SSCs displaying specific mRNA signatures. The current approach demonstrates the significant enrichment attained in the isolation of SSCs, with potential therein to enhance our understanding of bone cell biology and translational applications.
DNA, gold nanoparticles, human bone marrow, mRNA, skeletal stem cell, spherical nucleic acid
1936-0851
Xavier, Miguel
31fc5121-e816-4b46-91d9-18d271daa51b
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Lanham, Stuart
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Alexaki, Konstantina
4eb3b612-8a61-41cc-855e-7ccf917310f7
Matthews, Elloise
11178b9e-5173-4db3-8606-3ce4a5688e7f
El-Sagheer, Afaf H
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Xavier, Miguel
31fc5121-e816-4b46-91d9-18d271daa51b
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Lanham, Stuart
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Alexaki, Konstantina
4eb3b612-8a61-41cc-855e-7ccf917310f7
Matthews, Elloise
11178b9e-5173-4db3-8606-3ce4a5688e7f
El-Sagheer, Afaf H
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Xavier, Miguel, Kyriazi, Maria-Eleni, Lanham, Stuart, Alexaki, Konstantina, Matthews, Elloise, El-Sagheer, Afaf H, Brown, Tom, Kanaras, Antonios and Oreffo, Richard (2021) Enrichment of Skeletal Stem Cells from Human Bone Marrow Using Spherical Nucleic Acids. ACS Nano. (doi:10.1021/acsnano.0c10683).

Record type: Article

Abstract

Human bone marrow (BM)-derived stromal cells contain a population of skeletal stem cells (SSCs), with the capacity to differentiate along the osteogenic, adipogenic, and chondrogenic lineages, enabling their application to clinical therapies. However, current methods to isolate and enrich SSCs from human tissues remain, at best, challenging in the absence of a specific SSC marker. Unfortunately, none of the current proposed markers alone can isolate a homogeneous cell population with the ability to form bone, cartilage, and adipose tissue in humans. Here, we have designed DNA-gold nanoparticles able to identify and sort SSCs displaying specific mRNA signatures. The current approach demonstrates the significant enrichment attained in the isolation of SSCs, with potential therein to enhance our understanding of bone cell biology and translational applications.

Text
Enrichment of Skeletal Stem Cells - Accepted Manuscript
Restricted to Repository staff only until 22 March 2022.
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More information

Accepted/In Press date: 19 March 2021
Published date: 22 March 2021
Keywords: DNA, gold nanoparticles, human bone marrow, mRNA, skeletal stem cell, spherical nucleic acid

Identifiers

Local EPrints ID: 448009
URI: http://eprints.soton.ac.uk/id/eprint/448009
ISSN: 1936-0851
PURE UUID: 016bb957-43da-466f-a898-d8d86867ba2e
ORCID for Stuart Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for Konstantina Alexaki: ORCID iD orcid.org/0000-0002-8702-2696
ORCID for Antonios Kanaras: ORCID iD orcid.org/0000-0002-9847-6706
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 30 Mar 2021 16:32
Last modified: 12 May 2021 01:55

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