Microfluidic platform for serial mixing experiments with in operando nuclear magnetic resonance spectroscopy
Microfluidic platform for serial mixing experiments with in operando nuclear magnetic resonance spectroscopy
We present a microfluidic platform that allows in operando nuclear magnetic resonance (NMR) observation of serial mixing experiments. Gradually adding one reagent to another is a fundamental experimental modality, widely used to quantify equilibrium constants, for titrations, and in chemical kinetics studies. NMR provides a non-invasive means to quantify concentrations and to follow structural changes at the molecular level as a function of exchanged volume. Using active pneumatic valving on the microfluidic device directly inside an NMR spectrometer equipped with a transmission-line NMR microprobe, the system allows injection of aliquots and in situ mixing in a sample volume of less than 10 μL.
1598-1603
Plata, Marek
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Hale, William G
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Sharma, Manvendra
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Werner, Jorn
1b02513a-8310-4f4f-adac-dc2a466bd115
Utz, Marcel
c84ed64c-9e89-4051-af39-d401e423891b
21 April 2021
Plata, Marek
fa0e0a2a-0f55-4a6d-af90-761e5edbb2c1
Hale, William G
d98f9d64-00f9-447e-9783-185185a0b3c6
Sharma, Manvendra
e249236d-221d-4e59-8440-011f6863f891
Werner, Jorn
1b02513a-8310-4f4f-adac-dc2a466bd115
Utz, Marcel
c84ed64c-9e89-4051-af39-d401e423891b
Plata, Marek, Hale, William G, Sharma, Manvendra, Werner, Jorn and Utz, Marcel
(2021)
Microfluidic platform for serial mixing experiments with in operando nuclear magnetic resonance spectroscopy.
Lab on a Chip, 21 (8), .
(doi:10.1039/D0LC01100B).
Abstract
We present a microfluidic platform that allows in operando nuclear magnetic resonance (NMR) observation of serial mixing experiments. Gradually adding one reagent to another is a fundamental experimental modality, widely used to quantify equilibrium constants, for titrations, and in chemical kinetics studies. NMR provides a non-invasive means to quantify concentrations and to follow structural changes at the molecular level as a function of exchanged volume. Using active pneumatic valving on the microfluidic device directly inside an NMR spectrometer equipped with a transmission-line NMR microprobe, the system allows injection of aliquots and in situ mixing in a sample volume of less than 10 μL.
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More information
Accepted/In Press date: 2 February 2021
e-pub ahead of print date: 4 March 2021
Published date: 21 April 2021
Additional Information:
Funding Information:
This project was supported by the “TISuMR” project (grant no. 737043) funded through the Future and Emerging Technologies (FETOPEN) call of the EU Horizon 2020 research framework. MP gratefully acknowledges support from the Institute for Life Sciences, University of Southampton, Southampton, UK.
Publisher Copyright:
© The Royal Society of Chemistry 2021.
Identifiers
Local EPrints ID: 448093
URI: http://eprints.soton.ac.uk/id/eprint/448093
ISSN: 1473-0197
PURE UUID: 8cf27f4a-11fb-4bbb-ac96-03c9c81b0526
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Date deposited: 01 Apr 2021 15:58
Last modified: 06 Jun 2024 01:51
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Contributors
Author:
Marek Plata
Author:
William G Hale
Author:
Manvendra Sharma
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