Interactions of Pseudomonas aeruginosa with human corneal fibroblasts in vitro.
Interactions of Pseudomonas aeruginosa with human corneal fibroblasts in vitro.
Purpose: : To examine the cytopathic and immunological changes associated with live infection of primary human corneal fibroblasts with Pseudomonas aeruginosa.
Methods: : Human corneal fibroblasts (CF) were extracted from clinical samples, cultured to confluence in vitro and infected with various doses of Pseudomonas aeruginosa PAO-1. Bacterial association and invasion were quantified at 3, 6 and 9 hours and host cell death and cytokine profiles were assessed at 24 hours post challenge.
Results: : PAO-1 adhered to human CF in a dose and time-dependant manner and cytotoxic effects of bacterial interactions were observed within 9h. Preliminary data shows that constitutive levels of IL-8 are reduced with increasing infective dose, which reflects the pattern of cytotoxicity.
Conclusions: : We established an in vitro model of microbial keratitis and demonstrated the direct adherence of Pseudomonas aeruginosa to CF and the concurrent induction of host cell cytoxicity. The innate immune recognition of bacteria results in a decrease in resting levels of IL-8 in a dose dependent manner.
Elsahn, Ahmad
db5d3c4e-61a6-4069-8a20-64b077127ca6
Heath, C
d0399d29-306c-460e-b493-5f87a07ee0fa
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
March 2012
Elsahn, Ahmad
db5d3c4e-61a6-4069-8a20-64b077127ca6
Heath, C
d0399d29-306c-460e-b493-5f87a07ee0fa
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Elsahn, Ahmad, Heath, C, Christodoulides, Myron and Hossain, Parwez
(2012)
Interactions of Pseudomonas aeruginosa with human corneal fibroblasts in vitro.
Investigative Ophthalmology & Visual Science, 53 (14), [6136].
Record type:
Meeting abstract
Abstract
Purpose: : To examine the cytopathic and immunological changes associated with live infection of primary human corneal fibroblasts with Pseudomonas aeruginosa.
Methods: : Human corneal fibroblasts (CF) were extracted from clinical samples, cultured to confluence in vitro and infected with various doses of Pseudomonas aeruginosa PAO-1. Bacterial association and invasion were quantified at 3, 6 and 9 hours and host cell death and cytokine profiles were assessed at 24 hours post challenge.
Results: : PAO-1 adhered to human CF in a dose and time-dependant manner and cytotoxic effects of bacterial interactions were observed within 9h. Preliminary data shows that constitutive levels of IL-8 are reduced with increasing infective dose, which reflects the pattern of cytotoxicity.
Conclusions: : We established an in vitro model of microbial keratitis and demonstrated the direct adherence of Pseudomonas aeruginosa to CF and the concurrent induction of host cell cytoxicity. The innate immune recognition of bacteria results in a decrease in resting levels of IL-8 in a dose dependent manner.
This record has no associated files available for download.
More information
Published date: March 2012
Identifiers
Local EPrints ID: 448099
URI: http://eprints.soton.ac.uk/id/eprint/448099
ISSN: 0146-0404
PURE UUID: 8c5a6680-26f8-4338-b1fe-dff1ef3947f2
Catalogue record
Date deposited: 01 Apr 2021 15:58
Last modified: 17 Mar 2024 03:04
Export record
Contributors
Author:
Ahmad Elsahn
Author:
C Heath
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics