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Interactions of Pseudomonas aeruginosa with human corneal fibroblasts in vitro.

Interactions of Pseudomonas aeruginosa with human corneal fibroblasts in vitro.
Interactions of Pseudomonas aeruginosa with human corneal fibroblasts in vitro.
Purpose: : To examine the cytopathic and immunological changes associated with live infection of primary human corneal fibroblasts with Pseudomonas aeruginosa.

Methods: : Human corneal fibroblasts (CF) were extracted from clinical samples, cultured to confluence in vitro and infected with various doses of Pseudomonas aeruginosa PAO-1. Bacterial association and invasion were quantified at 3, 6 and 9 hours and host cell death and cytokine profiles were assessed at 24 hours post challenge.

Results: : PAO-1 adhered to human CF in a dose and time-dependant manner and cytotoxic effects of bacterial interactions were observed within 9h. Preliminary data shows that constitutive levels of IL-8 are reduced with increasing infective dose, which reflects the pattern of cytotoxicity.

Conclusions: : We established an in vitro model of microbial keratitis and demonstrated the direct adherence of Pseudomonas aeruginosa to CF and the concurrent induction of host cell cytoxicity. The innate immune recognition of bacteria results in a decrease in resting levels of IL-8 in a dose dependent manner.
0146-0404
Elsahn, Ahmad
db5d3c4e-61a6-4069-8a20-64b077127ca6
Heath, C
d0399d29-306c-460e-b493-5f87a07ee0fa
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Elsahn, Ahmad
db5d3c4e-61a6-4069-8a20-64b077127ca6
Heath, C
d0399d29-306c-460e-b493-5f87a07ee0fa
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51

Elsahn, Ahmad, Heath, C, Christodoulides, Myron and Hossain, Parwez (2012) Interactions of Pseudomonas aeruginosa with human corneal fibroblasts in vitro. Investigative Ophthalmology & Visual Science, 53 (14), [6136].

Record type: Meeting abstract

Abstract

Purpose: : To examine the cytopathic and immunological changes associated with live infection of primary human corneal fibroblasts with Pseudomonas aeruginosa.

Methods: : Human corneal fibroblasts (CF) were extracted from clinical samples, cultured to confluence in vitro and infected with various doses of Pseudomonas aeruginosa PAO-1. Bacterial association and invasion were quantified at 3, 6 and 9 hours and host cell death and cytokine profiles were assessed at 24 hours post challenge.

Results: : PAO-1 adhered to human CF in a dose and time-dependant manner and cytotoxic effects of bacterial interactions were observed within 9h. Preliminary data shows that constitutive levels of IL-8 are reduced with increasing infective dose, which reflects the pattern of cytotoxicity.

Conclusions: : We established an in vitro model of microbial keratitis and demonstrated the direct adherence of Pseudomonas aeruginosa to CF and the concurrent induction of host cell cytoxicity. The innate immune recognition of bacteria results in a decrease in resting levels of IL-8 in a dose dependent manner.

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More information

Published date: March 2012

Identifiers

Local EPrints ID: 448099
URI: http://eprints.soton.ac.uk/id/eprint/448099
ISSN: 0146-0404
PURE UUID: 8c5a6680-26f8-4338-b1fe-dff1ef3947f2
ORCID for Myron Christodoulides: ORCID iD orcid.org/0000-0002-9663-4731
ORCID for Parwez Hossain: ORCID iD orcid.org/0000-0002-3131-2395

Catalogue record

Date deposited: 01 Apr 2021 15:58
Last modified: 13 Apr 2021 01:41

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Contributors

Author: Ahmad Elsahn
Author: C Heath
Author: Parwez Hossain ORCID iD

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