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ATR based infrared spectroscopy for the diagnosis of neonatal respiratory distress syndrome

ATR based infrared spectroscopy for the diagnosis of neonatal respiratory distress syndrome
ATR based infrared spectroscopy for the diagnosis of neonatal respiratory distress syndrome
Optical spectroscopy offers a potential non-invasive, label free and rapid method to assist clinicians to diagnose diseases for which biomarkers are known. Neonatal respiratory distress syndrome (nRDS) diagnosis in preterm infants is known to be correlated with the lecithin/sphingomyelin ratio (LS ratio) in gastric aspirates, with a ratio less than 2.2 indicating that surfactant replacement therapy is needed. Currently no widespread method exists that can give clinically relevant answers in less than 2 hours from the point of sample collection as it is difficult to identify those who could benefit from prompt surfactant treatment. Various LS ratios were generated using pure dipalmitoylphosphatidylcholine (DPPC) and sphingomyelin (SM) dissolved in dichloromethane and infrared spectra generated using Attenuated Total Reflection (ATR) assisted Fourier Transform InfraRed spectrometry (FTIR). Subsequent analysis obtained the LS ratio using the spectra alone. Further, we demonstrate the application of principal component regression (PCR) and partial least squares (PLS) fits to measured spectra to assist in the determination of the LS ratio using a model trained with multiple runs of the different batches of the same concentration.
ATR-FTIR, Biomarker diagnosis, Biomedical diagnostics, Mid-IR spectroscopy, NRDS
SPIE
Ahmed, Waseem
4326b5dd-ca37-4ea0-bd27-294f2ef011e6
Veluthandath, Aneesh Vincent
6a183413-e10f-4374-bc64-a33bf7fd9cfa
Madsen, Jens
21cf021a-4bd4-4cdf-9db7-52ff2459663d
Clark, Howard W.
a5fd860a-269b-4e37-9075-73f94eb00471
Postle, Antony D.
4f5e96dc-ccaa-4ca5-9807-0612fb6294fc
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Murugan, Ganapathy Senthil
a867686e-0535-46cc-ad85-c2342086b25b
Ahmed, Waseem
4326b5dd-ca37-4ea0-bd27-294f2ef011e6
Veluthandath, Aneesh Vincent
6a183413-e10f-4374-bc64-a33bf7fd9cfa
Madsen, Jens
21cf021a-4bd4-4cdf-9db7-52ff2459663d
Clark, Howard W.
a5fd860a-269b-4e37-9075-73f94eb00471
Postle, Antony D.
4f5e96dc-ccaa-4ca5-9807-0612fb6294fc
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Murugan, Ganapathy Senthil
a867686e-0535-46cc-ad85-c2342086b25b

Ahmed, Waseem, Veluthandath, Aneesh Vincent, Madsen, Jens, Clark, Howard W., Postle, Antony D., Wilkinson, James S. and Murugan, Ganapathy Senthil (2021) ATR based infrared spectroscopy for the diagnosis of neonatal respiratory distress syndrome. In Proceedings Volume 11651: Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics. vol. 11651, SPIE. 6 pp . (doi:10.1117/12.2578818).

Record type: Conference or Workshop Item (Paper)

Abstract

Optical spectroscopy offers a potential non-invasive, label free and rapid method to assist clinicians to diagnose diseases for which biomarkers are known. Neonatal respiratory distress syndrome (nRDS) diagnosis in preterm infants is known to be correlated with the lecithin/sphingomyelin ratio (LS ratio) in gastric aspirates, with a ratio less than 2.2 indicating that surfactant replacement therapy is needed. Currently no widespread method exists that can give clinically relevant answers in less than 2 hours from the point of sample collection as it is difficult to identify those who could benefit from prompt surfactant treatment. Various LS ratios were generated using pure dipalmitoylphosphatidylcholine (DPPC) and sphingomyelin (SM) dissolved in dichloromethane and infrared spectra generated using Attenuated Total Reflection (ATR) assisted Fourier Transform InfraRed spectrometry (FTIR). Subsequent analysis obtained the LS ratio using the spectra alone. Further, we demonstrate the application of principal component regression (PCR) and partial least squares (PLS) fits to measured spectra to assist in the determination of the LS ratio using a model trained with multiple runs of the different batches of the same concentration.

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More information

Published date: 5 March 2021
Keywords: ATR-FTIR, Biomarker diagnosis, Biomedical diagnostics, Mid-IR spectroscopy, NRDS

Identifiers

Local EPrints ID: 448153
URI: http://eprints.soton.ac.uk/id/eprint/448153
PURE UUID: cb593d34-19f8-4c95-919f-8d804b66236b
ORCID for Aneesh Vincent Veluthandath: ORCID iD orcid.org/0000-0003-4306-6723
ORCID for James S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697
ORCID for Ganapathy Senthil Murugan: ORCID iD orcid.org/0000-0002-2733-3273

Catalogue record

Date deposited: 13 Apr 2021 16:31
Last modified: 22 Jun 2021 01:59

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Contributors

Author: Waseem Ahmed
Author: Jens Madsen
Author: Howard W. Clark
Author: Antony D. Postle

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