Epitopes of enzyme digested retinal S-antigen in experimental autoimmune uveitis (EAU)

Brown, P A, Dua, H.S., Hossain, Parwez and Forrester, J (1989) Epitopes of enzyme digested retinal S-antigen in experimental autoimmune uveitis (EAU). The Journal of Pathology, 158 (4), A355. (doi:10.1002/path.1711580412).

Abstract

Sympathetic ophthalmitis, a form of chronic posterior uveitis leads to permanent visual impairment. Experimental Autoimmune Uveitis (EAU) is a good animal model of the disease and is produced  by injection of S-antigen, a soluble photoreceptor protein in Freund's adjuvant. Sympathetic ophthalmitis rarely occurs in infected eyes and this suggests that degradation of the antigen by bacterial proteases may protect against the disease. We used various enzymes - papain, trypsin and Staph. aureus V8 - to investigate whether the bacterial protiase would destroy the antigenic epitopes of S-antigen. S-antigen was digested by the enzymes and separated by SDS-PAGE. lmmunobiots were performed using a rat antibovin S-antigen monoclonal antibody (S2.4 C5). The papain and trypsin epitopes stain with the antibody whilst none of the Staph. aureus V8 protease epitopes slain. These results will be discussed in relation to the effects of peptide fragments of S-antigen In the induction of EAU.

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Contributors

Author: Parwez Hossain

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