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Epitopes of enzyme digested retinal S-antigen in experimental autoimmune uveitis (EAU)

Epitopes of enzyme digested retinal S-antigen in experimental autoimmune uveitis (EAU)
Epitopes of enzyme digested retinal S-antigen in experimental autoimmune uveitis (EAU)
Sympathetic ophthalmitis, a form of chronic posterior uveitis leads to permanent visual impairment. Experimental Autoimmune Uveitis (EAU) is a good animal model of the disease and is produced  by injection of S-antigen, a soluble photoreceptor protein in Freund's adjuvant. Sympathetic ophthalmitis rarely occurs in infected eyes and this suggests that degradation of the antigen by bacterial proteases may protect against the disease. We used various enzymes - papain, trypsin and Staph. aureus V8 - to investigate whether the bacterial protiase would destroy the antigenic epitopes of S-antigen. S-antigen was digested by the enzymes and separated by SDS-PAGE. lmmunobiots were performed using a rat antibovin S-antigen monoclonal antibody (S2.4 C5). The papain and trypsin epitopes stain with the antibody whilst none of the Staph. aureus V8 protease epitopes slain. These results will be discussed in relation to the effects of peptide fragments of S-antigen In the induction of EAU.
1096-9896
A355
Brown, P A
99ae5ab1-0e3e-4db5-9103-e813392f4621
Dua, H.S.
ce0ea09e-99dd-4335-b048-e0c0261bbddc
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Forrester, J
790c966a-5b4f-4e3a-ad20-694a69b76c8c
Brown, P A
99ae5ab1-0e3e-4db5-9103-e813392f4621
Dua, H.S.
ce0ea09e-99dd-4335-b048-e0c0261bbddc
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Forrester, J
790c966a-5b4f-4e3a-ad20-694a69b76c8c

Brown, P A, Dua, H.S., Hossain, Parwez and Forrester, J (1989) Epitopes of enzyme digested retinal S-antigen in experimental autoimmune uveitis (EAU). The Journal of Pathology, 158 (4), A355. (doi:10.1002/path.1711580412).

Record type: Article

Abstract

Sympathetic ophthalmitis, a form of chronic posterior uveitis leads to permanent visual impairment. Experimental Autoimmune Uveitis (EAU) is a good animal model of the disease and is produced  by injection of S-antigen, a soluble photoreceptor protein in Freund's adjuvant. Sympathetic ophthalmitis rarely occurs in infected eyes and this suggests that degradation of the antigen by bacterial proteases may protect against the disease. We used various enzymes - papain, trypsin and Staph. aureus V8 - to investigate whether the bacterial protiase would destroy the antigenic epitopes of S-antigen. S-antigen was digested by the enzymes and separated by SDS-PAGE. lmmunobiots were performed using a rat antibovin S-antigen monoclonal antibody (S2.4 C5). The papain and trypsin epitopes stain with the antibody whilst none of the Staph. aureus V8 protease epitopes slain. These results will be discussed in relation to the effects of peptide fragments of S-antigen In the induction of EAU.

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Published date: August 1989

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Local EPrints ID: 448155
URI: http://eprints.soton.ac.uk/id/eprint/448155
ISSN: 1096-9896
PURE UUID: da40e83e-211a-4698-9ca7-7e916bfbf957
ORCID for Parwez Hossain: ORCID iD orcid.org/0000-0002-3131-2395

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Date deposited: 13 Apr 2021 16:31
Last modified: 08 Sep 2021 01:41

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Contributors

Author: P A Brown
Author: H.S. Dua
Author: Parwez Hossain ORCID iD
Author: J Forrester

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