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Pseudomonas aeruginosaactivates components of the inflammasome complex in human corneal fibroblast

Pseudomonas aeruginosaactivates components of the inflammasome complex in human corneal fibroblast
Pseudomonas aeruginosaactivates components of the inflammasome complex in human corneal fibroblast
Microbial keratitis is a principal cause of visual impairment andblindness worldwide. Pseudomonas aeruginosa has been described asthe leading Gram-negative bacteria causative agent of corneal infec-tions associated with contact lens and ocular trauma. In front of theexternal environment, the cornea provides a defensive barrier and itis important in the host innate immune response to microbial inva-sion. Studies in mice have revealed that the activation of multipro-tein complexes called inflammasomes, in response to the bacterialinfection, plays an important role in the pathogenesis and in the cor-neal tissue damage. The inflammasome complex contains signallingmolecules, e.g. ASC, NLRs and caspases, that assemble in response ofpathogen associated molecular patterns (PAMPs) recognition andlead to secretion of IL-1B and IL-18. In our study, we have usedqPCR and western bloting to investigate the activation of differentinflammasome molecules in primary human corneal fibroblastinfected with P. aeruginosa PAO1 strain. In addition, we have inves-tigated how the non-functionality of P. aeruginosa virulent factorsmodify the corneal immune signalling cascade and inflammasomeactivation and the release of pro-inflammatory cytokines. Our dataalso indicate that P. aeruginosa influences the nature of caspase acti-vation and this is dependent of the expression of particular virulencefactors.
0019-2805
111
Cendra Gascon, Maria del mar DM
150d34a4-4598-4138-96c0-06f3f338cca2
Elsahn, Ahmad
db5d3c4e-61a6-4069-8a20-64b077127ca6
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Cendra Gascon, Maria del mar DM
150d34a4-4598-4138-96c0-06f3f338cca2
Elsahn, Ahmad
db5d3c4e-61a6-4069-8a20-64b077127ca6
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51

Cendra Gascon, Maria del mar DM, Elsahn, Ahmad, Christodoulides, Myron and Hossain, Parwez (2014) Pseudomonas aeruginosaactivates components of the inflammasome complex in human corneal fibroblast. Immunology, 143 ((suppl 2)), 111. (doi:10.1111/imm.12406).

Record type: Meeting abstract

Abstract

Microbial keratitis is a principal cause of visual impairment andblindness worldwide. Pseudomonas aeruginosa has been described asthe leading Gram-negative bacteria causative agent of corneal infec-tions associated with contact lens and ocular trauma. In front of theexternal environment, the cornea provides a defensive barrier and itis important in the host innate immune response to microbial inva-sion. Studies in mice have revealed that the activation of multipro-tein complexes called inflammasomes, in response to the bacterialinfection, plays an important role in the pathogenesis and in the cor-neal tissue damage. The inflammasome complex contains signallingmolecules, e.g. ASC, NLRs and caspases, that assemble in response ofpathogen associated molecular patterns (PAMPs) recognition andlead to secretion of IL-1B and IL-18. In our study, we have usedqPCR and western bloting to investigate the activation of differentinflammasome molecules in primary human corneal fibroblastinfected with P. aeruginosa PAO1 strain. In addition, we have inves-tigated how the non-functionality of P. aeruginosa virulent factorsmodify the corneal immune signalling cascade and inflammasomeactivation and the release of pro-inflammatory cytokines. Our dataalso indicate that P. aeruginosa influences the nature of caspase acti-vation and this is dependent of the expression of particular virulencefactors.

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More information

e-pub ahead of print date: 28 November 2014
Published date: December 2014

Identifiers

Local EPrints ID: 448281
URI: http://eprints.soton.ac.uk/id/eprint/448281
ISSN: 0019-2805
PURE UUID: 1107b14e-8a49-4793-b9f5-0138b2b36501
ORCID for Myron Christodoulides: ORCID iD orcid.org/0000-0002-9663-4731
ORCID for Parwez Hossain: ORCID iD orcid.org/0000-0002-3131-2395

Catalogue record

Date deposited: 19 Apr 2021 16:30
Last modified: 20 Apr 2021 01:41

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Contributors

Author: Maria del mar DM Cendra Gascon
Author: Ahmad Elsahn
Author: Parwez Hossain ORCID iD

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