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Data from: Acute exposure to diesel exhaust induces central nervous system stress and altered learning and memory in honey bees

Data from: Acute exposure to diesel exhaust induces central nervous system stress and altered learning and memory in honey bees
Data from: Acute exposure to diesel exhaust induces central nervous system stress and altered learning and memory in honey bees
For effective foraging, many insect pollinators rely on the ability to learn and recall floral odours, behaviours that are associated with a complex suite of cellular processes. Here, we investigated how acute exposure to a high-dose of diesel exhaust (containing 19.8 and 17.5 ppm of NO and NO2, respectively) affected associative learning behaviour of honey bees (Apis mellifera) and expression of a ubiquitous heat shock protein, HSP70, in their central nervous system (CNS). To determine whether exposure to diesel exhaust would alter their tolerance to a subsequent abiotic stress, we further subjected individuals to heat stress. Diesel exhaust exposure decreased honey bees’ ability to learn and recall a conditioned odour stimulus. Whilst there was no significant difference in CNS HSP70 expression between honey bees exposed to either diesel exhaust or clean air across the entire duration of the experiment (3.5 h), there was a significant effect of time and a significant interaction between exposure treatment and time. This interaction was investigated using correlation analyses, which demonstrated that only in the diesel exhaust exposed honey bees was there a significant positive correlation between HSP70 expression and time. Furthermore, there was a 44% reduction in honey bee individuals that were able to recall the odour 72 h after diesel exposure compared with clean air control individuals. Moreover, diesel exhaust affected A. mellifera in a way that reduced their ability to survive a second subsequent stressor. Such negative effects of air pollution on learning, recall, and stress tolerance has potential to reduce foraging efficiency and pollination success of individual honey bees.,Reitmayer_et_al_2019_honey_bee_diesel_pollution_Sci_RepThis zip file includes five separate .csv files. Each file contains the data used to create one graph and it's accompanying statistical analysis for the following paper: Reitmayer CM, Ryalls JMW, Farthing E, Jackson CW, Girling RD, Newman TA. 2019. Acute exposure to diesel exhaust induces central nervous system stress and altered learning and memory in honey bees. Sci. Rep.All_manuscript_data.zip,
DRYAD
Reitmayer, Christine M.
0339b876-4b43-4aac-8057-46fc7ea855e7
Ryalls, James M. W.
c7a931d9-aec4-4a8c-a522-d6d089b371ce
Farthing, Emily
121f4586-6ec7-4ee4-af80-97f45ebba4dd
Jackson, Christopher W.
ab14e7be-1b25-4425-9e8f-6ccee5b984a8
Girling, Robbie D.
1044dcd8-9b1a-4f9c-bd42-7aa960de5470
Newman, Tracey A.
322290cb-2e9c-445d-a047-00b1bea39a25
Reitmayer, Christine M.
0339b876-4b43-4aac-8057-46fc7ea855e7
Ryalls, James M. W.
c7a931d9-aec4-4a8c-a522-d6d089b371ce
Farthing, Emily
121f4586-6ec7-4ee4-af80-97f45ebba4dd
Jackson, Christopher W.
ab14e7be-1b25-4425-9e8f-6ccee5b984a8
Girling, Robbie D.
1044dcd8-9b1a-4f9c-bd42-7aa960de5470
Newman, Tracey A.
322290cb-2e9c-445d-a047-00b1bea39a25

(2019) Data from: Acute exposure to diesel exhaust induces central nervous system stress and altered learning and memory in honey bees. DRYAD doi:10.5061/dryad.vb27ks3 [Dataset]

Record type: Dataset

Abstract

For effective foraging, many insect pollinators rely on the ability to learn and recall floral odours, behaviours that are associated with a complex suite of cellular processes. Here, we investigated how acute exposure to a high-dose of diesel exhaust (containing 19.8 and 17.5 ppm of NO and NO2, respectively) affected associative learning behaviour of honey bees (Apis mellifera) and expression of a ubiquitous heat shock protein, HSP70, in their central nervous system (CNS). To determine whether exposure to diesel exhaust would alter their tolerance to a subsequent abiotic stress, we further subjected individuals to heat stress. Diesel exhaust exposure decreased honey bees’ ability to learn and recall a conditioned odour stimulus. Whilst there was no significant difference in CNS HSP70 expression between honey bees exposed to either diesel exhaust or clean air across the entire duration of the experiment (3.5 h), there was a significant effect of time and a significant interaction between exposure treatment and time. This interaction was investigated using correlation analyses, which demonstrated that only in the diesel exhaust exposed honey bees was there a significant positive correlation between HSP70 expression and time. Furthermore, there was a 44% reduction in honey bee individuals that were able to recall the odour 72 h after diesel exposure compared with clean air control individuals. Moreover, diesel exhaust affected A. mellifera in a way that reduced their ability to survive a second subsequent stressor. Such negative effects of air pollution on learning, recall, and stress tolerance has potential to reduce foraging efficiency and pollination success of individual honey bees.,Reitmayer_et_al_2019_honey_bee_diesel_pollution_Sci_RepThis zip file includes five separate .csv files. Each file contains the data used to create one graph and it's accompanying statistical analysis for the following paper: Reitmayer CM, Ryalls JMW, Farthing E, Jackson CW, Girling RD, Newman TA. 2019. Acute exposure to diesel exhaust induces central nervous system stress and altered learning and memory in honey bees. Sci. Rep.All_manuscript_data.zip,

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More information

Published date: 1 January 2019

Identifiers

Local EPrints ID: 448462
URI: http://eprints.soton.ac.uk/id/eprint/448462
PURE UUID: 2e55f069-1ac9-42b0-8af0-ae63fc943486
ORCID for Tracey A. Newman: ORCID iD orcid.org/0000-0002-3727-9258

Catalogue record

Date deposited: 22 Apr 2021 16:47
Last modified: 23 Apr 2021 01:34

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Contributors

Contributor: Christine M. Reitmayer
Contributor: James M. W. Ryalls
Contributor: Emily Farthing
Contributor: Christopher W. Jackson
Contributor: Robbie D. Girling
Contributor: Tracey A. Newman ORCID iD

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