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Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja

Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja
Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja
Extant deep-sea invertebrate fauna represent both ancient and recent invasions from shallow-water habitats. Hydrostatic pressure may present a significant physiological challenge to organisms seeking to colonize deeper waters or migrate ontogenetically. Pressure may be a key factor contributing to bottlenecks in the radiation of taxa and potentially drive speciation. Here, we assess shifts in the tolerance of hydrostatic pressure through early ontogeny of the northern stone crab Lithodes maja, which occupies a depth range of 4–790 m in the North Atlantic. The zoea I, megalopa and crab I stages were exposed to hydrostatic pressures up to 30.0 MPa (equivalent of 3000 m depth), and the relative fold change of genes putatively coding for the N-methyl-d-aspartate receptor-regulated protein 1 (narg gene), two heat-shock protein 70 kDa (HSP70) isoforms and mitochondrial Citrate Synthase (CS gene) were measured. This study finds a significant increase in the relative expression of the CS and hsp70a genes with increased hydrostatic pressure in the zoea I stage, and an increase in the relative expression of all genes with increased hydrostatic pressure in the megalopa and crab I stages. Transcriptional responses are corroborated by patterns in respiratory rates in response to hydrostatic pressure in all stages. These results suggest a decrease in the acute high-pressure tolerance limit as ontogeny advances, as reflected by a shift in the hydrostatic pressure at which significant differences are observed.,Narg FASTA files for alignmentFASTA format files used to derive the narg gene alignmentNarg_FASTA_files_for_alignment.txtClustalO narg alignmentClustal Omega alignment file for the narg geneclustalo_narg_alignment.clustalRpl8 FASTA files for alignmentFASTA files used to derive the rpl8 alignmentRpl8_FASTA_files_for_alignment.txtClustalO rpl8 alignmentClustal Omega download file for rpl8 alignmentclustalo_rpl8_alignment.clustalTubulin FASTA files for alignmentFASTA files used to derives Tubulin gene alignmentTubulin_FASTA_files_for_alignment.txtClustalO tubulin alignmentClustal Omega download for tubulin gene alignmentclustalo_tubulin_alignment.clustalEef1a FASTA file alignmentsFASTA files used to derive eef1a gene alignmentEef1a_FASTA_file_alignments.txtClustalO eef1a alignmentClustal Omega download for eef1a gene alignmentclustalo_eef1a_alignment.clustalCS FASTA files for alignmentFASTA files used to derive CS gene alignmentCs_FASTA_files_for_alignment.txtClustalO cs alignmentClustal Omega download file for CS gene alignmentclustalo_cs_alignment.clustalHsp70a FASTA files for alignmentFASTA files used to derive HSP70a gene alignmentHsp70a_FASTA_files_for_alignment.txtClustalO hsp70a alignmentClustal Omega download file for hsp70a gene alignmentclustalo_hsp70a_alignment.clustalHsp70b FASTA files for alignmentFASTA files used to derive hsp70b gene alignmentHsp70b_FASTA_files_for_alignment.txtClustalO hsp70b alignmentClustal Omega download for hsp70b gene alignmentclustalo_hsp70b_alignment.clustal,
DRYAD
Munro, Catriona
fcf87280-e033-4e41-8d50-2cba2a16869d
Morris, James P.
7060ae12-d0fd-41a5-89df-83870b59be31
Brown, Alastair
909f34db-bc9c-403f-ba8f-31aee1c00161
Hauton, Chris
6ced1eb9-f074-45b1-98ec-3101b80dd527
Thatje, Sven
f1011fe3-1048-40c0-97c1-e93b796e6533
Munro, Catriona
fcf87280-e033-4e41-8d50-2cba2a16869d
Morris, James P.
7060ae12-d0fd-41a5-89df-83870b59be31
Brown, Alastair
909f34db-bc9c-403f-ba8f-31aee1c00161
Hauton, Chris
6ced1eb9-f074-45b1-98ec-3101b80dd527
Thatje, Sven
f1011fe3-1048-40c0-97c1-e93b796e6533

Hauton, Chris (2015) Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja. DRYAD doi:10.5061/dryad.d6t40 [Dataset]

Record type: Dataset

Abstract

Extant deep-sea invertebrate fauna represent both ancient and recent invasions from shallow-water habitats. Hydrostatic pressure may present a significant physiological challenge to organisms seeking to colonize deeper waters or migrate ontogenetically. Pressure may be a key factor contributing to bottlenecks in the radiation of taxa and potentially drive speciation. Here, we assess shifts in the tolerance of hydrostatic pressure through early ontogeny of the northern stone crab Lithodes maja, which occupies a depth range of 4–790 m in the North Atlantic. The zoea I, megalopa and crab I stages were exposed to hydrostatic pressures up to 30.0 MPa (equivalent of 3000 m depth), and the relative fold change of genes putatively coding for the N-methyl-d-aspartate receptor-regulated protein 1 (narg gene), two heat-shock protein 70 kDa (HSP70) isoforms and mitochondrial Citrate Synthase (CS gene) were measured. This study finds a significant increase in the relative expression of the CS and hsp70a genes with increased hydrostatic pressure in the zoea I stage, and an increase in the relative expression of all genes with increased hydrostatic pressure in the megalopa and crab I stages. Transcriptional responses are corroborated by patterns in respiratory rates in response to hydrostatic pressure in all stages. These results suggest a decrease in the acute high-pressure tolerance limit as ontogeny advances, as reflected by a shift in the hydrostatic pressure at which significant differences are observed.,Narg FASTA files for alignmentFASTA format files used to derive the narg gene alignmentNarg_FASTA_files_for_alignment.txtClustalO narg alignmentClustal Omega alignment file for the narg geneclustalo_narg_alignment.clustalRpl8 FASTA files for alignmentFASTA files used to derive the rpl8 alignmentRpl8_FASTA_files_for_alignment.txtClustalO rpl8 alignmentClustal Omega download file for rpl8 alignmentclustalo_rpl8_alignment.clustalTubulin FASTA files for alignmentFASTA files used to derives Tubulin gene alignmentTubulin_FASTA_files_for_alignment.txtClustalO tubulin alignmentClustal Omega download for tubulin gene alignmentclustalo_tubulin_alignment.clustalEef1a FASTA file alignmentsFASTA files used to derive eef1a gene alignmentEef1a_FASTA_file_alignments.txtClustalO eef1a alignmentClustal Omega download for eef1a gene alignmentclustalo_eef1a_alignment.clustalCS FASTA files for alignmentFASTA files used to derive CS gene alignmentCs_FASTA_files_for_alignment.txtClustalO cs alignmentClustal Omega download file for CS gene alignmentclustalo_cs_alignment.clustalHsp70a FASTA files for alignmentFASTA files used to derive HSP70a gene alignmentHsp70a_FASTA_files_for_alignment.txtClustalO hsp70a alignmentClustal Omega download file for hsp70a gene alignmentclustalo_hsp70a_alignment.clustalHsp70b FASTA files for alignmentFASTA files used to derive hsp70b gene alignmentHsp70b_FASTA_files_for_alignment.txtClustalO hsp70b alignmentClustal Omega download for hsp70b gene alignmentclustalo_hsp70b_alignment.clustal,

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More information

Published date: 1 January 2015

Identifiers

Local EPrints ID: 448667
URI: http://eprints.soton.ac.uk/id/eprint/448667
PURE UUID: 187ebdd2-5e06-48a4-9ed1-938db3fccea3

Catalogue record

Date deposited: 29 Apr 2021 16:32
Last modified: 25 May 2022 17:11

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Contributors

Contributor: Catriona Munro
Contributor: James P. Morris
Contributor: Alastair Brown
Creator: Chris Hauton
Contributor: Sven Thatje

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