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Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution

Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution
Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution
Magnetic resonance imaging is hindered by inherently low sensitivity, which limits the method for the most part to observing water molecules in the body. Hyperpolarized molecules exhibit strongly enhanced MRI signals which opens the door for imaging low-concentration species in vivo. Biomolecules can be hyperpolarized and injected into a patient allowing for metabolism to be tracked in real time, greatly expanding the information available to the radiologist. Parahydrogen-induced polarization (PHIP) is a hyperpolarization method renowned for its low cost and accessibility, but is generally limited by low polarization levels, modest molecular concentrations, and contamination by polarization reagents. In this work we overcome these drawbacks in the production of PHIP-polarized [1-13C]fumarate, a biomarker of cell necrosis in metabolic 13C MRI.
Biomarker, Hyperpolarization, MRI, Metabolism, Parahydrogen
0027-8424
1-6
Knecht, Stephan
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Blanchard, John W.
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Barskiy, Danila
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Cavallari, Eleonora
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Dagys, Laurynas
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Van Dyke, Erik
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Tsukanov, Maksim
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Bliemel, Bea
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Münnemann, Kerstin
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Aime, Silvio
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Reineri, Francesca
d68b60ad-5730-4ac2-9b78-eeeb8aa90711
Levitt, Malcolm H.
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Buntkowsky, Gerd
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Pines, Alexander
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Blümler, Peter
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Budker, Dmitry
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Eills, James
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Knecht, Stephan
7618faf5-ce64-4c20-9b57-9c30d300cfb7
Blanchard, John W.
3bb7de6e-f147-4527-9b1c-fbf8cb138f47
Barskiy, Danila
570074be-bef1-49e3-95a7-b82d701dc7ee
Cavallari, Eleonora
c65fe867-7f4b-451e-9317-e8bc481ff26e
Dagys, Laurynas
0de61597-b152-4bee-a934-123a9d2de883
Van Dyke, Erik
671a1174-83df-4658-ab1e-97d256f6b487
Tsukanov, Maksim
264d4e5b-5c91-485b-ba82-b2c98ceb7496
Bliemel, Bea
8a7f64c2-3b22-485c-b4c1-29461116fbac
Münnemann, Kerstin
431726d4-549d-417c-9fe9-0446201caa9e
Aime, Silvio
bed88969-3890-4d0b-8640-537a9d299c12
Reineri, Francesca
d68b60ad-5730-4ac2-9b78-eeeb8aa90711
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Buntkowsky, Gerd
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Pines, Alexander
516303cb-c05b-4bc6-b276-18b262e78ad9
Blümler, Peter
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Budker, Dmitry
2d8147c6-82b8-4e9a-8871-cb0d49b9cbea
Eills, James
23130b21-68fa-4c8b-9399-e55f2e71ef36

Knecht, Stephan, Blanchard, John W., Barskiy, Danila, Cavallari, Eleonora, Dagys, Laurynas, Van Dyke, Erik, Tsukanov, Maksim, Bliemel, Bea, Münnemann, Kerstin, Aime, Silvio, Reineri, Francesca, Levitt, Malcolm H., Buntkowsky, Gerd, Pines, Alexander, Blümler, Peter, Budker, Dmitry and Eills, James (2021) Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution. Proceedings of the National Academy of Sciences, 118 (13), 1-6, [e2025383118]. (doi:10.1073/pnas.2025383118).

Record type: Article

Abstract

Magnetic resonance imaging is hindered by inherently low sensitivity, which limits the method for the most part to observing water molecules in the body. Hyperpolarized molecules exhibit strongly enhanced MRI signals which opens the door for imaging low-concentration species in vivo. Biomolecules can be hyperpolarized and injected into a patient allowing for metabolism to be tracked in real time, greatly expanding the information available to the radiologist. Parahydrogen-induced polarization (PHIP) is a hyperpolarization method renowned for its low cost and accessibility, but is generally limited by low polarization levels, modest molecular concentrations, and contamination by polarization reagents. In this work we overcome these drawbacks in the production of PHIP-polarized [1-13C]fumarate, a biomarker of cell necrosis in metabolic 13C MRI.

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fumarate_precip_PNAS_accepted - Accepted Manuscript
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More information

Accepted/In Press date: 17 February 2021
e-pub ahead of print date: 22 March 2021
Published date: 30 March 2021
Keywords: Biomarker, Hyperpolarization, MRI, Metabolism, Parahydrogen

Identifiers

Local EPrints ID: 448934
URI: http://eprints.soton.ac.uk/id/eprint/448934
ISSN: 0027-8424
PURE UUID: 96f36dda-3af0-4ced-94b9-f4c071ce61a1
ORCID for Malcolm H. Levitt: ORCID iD orcid.org/0000-0001-9878-1180

Catalogue record

Date deposited: 11 May 2021 16:48
Last modified: 26 Nov 2021 02:44

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Contributors

Author: Stephan Knecht
Author: John W. Blanchard
Author: Danila Barskiy
Author: Eleonora Cavallari
Author: Laurynas Dagys
Author: Erik Van Dyke
Author: Maksim Tsukanov
Author: Bea Bliemel
Author: Kerstin Münnemann
Author: Silvio Aime
Author: Francesca Reineri
Author: Gerd Buntkowsky
Author: Alexander Pines
Author: Peter Blümler
Author: Dmitry Budker
Author: James Eills

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