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Neuropixels 2.0: a miniaturized high-density probe for stable, long-term brain recordings

Neuropixels 2.0: a miniaturized high-density probe for stable, long-term brain recordings
Neuropixels 2.0: a miniaturized high-density probe for stable, long-term brain recordings

Measuring the dynamics of neural processing across time scales requires following the spiking of thousands of individual neurons over milliseconds and months. To address this need, we introduce the Neuropixels 2.0 probe together with newly designed analysis algorithms. The probe has more than 5000 sites and is miniaturized to facilitate chronic implants in small mammals and recording during unrestrained behavior. High-quality recordings over long time scales were reliably obtained in mice and rats in six laboratories. Improved site density and arrangement combined with newly created data processing methods enable automatic post hoc correction for brain movements, allowing recording from the same neurons for more than 2 months. These probes and algorithms enable stable recordings from thousands of sites during free behavior, even in small animals such as mice.

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Park, Junchol
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Wang, Shiwei
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Steinmetz, Nicholas A., Aydin, Cagatay, Lebedeva, Anna, Okun, Michael, Pachitariu, Marius, Bauza, Marius, Beau, Maxime, Bhagat, Jai, Böhm, Claudia, Broux, Martijn, Chen, Susu, Colonell, Jennifer, Gardner, Richard J., Karsh, Bill, Kloosterman, Fabian, Kostadinov, Dimitar, Mora-Lopez, Carolina, O’Callaghan, John, Park, Junchol, Putzeys, Jan, Sauerbrei, Britton, Daal, Rik J. J. van, Vollan, Abraham Z., Wang, Shiwei, Welkenhuysen, Marleen, Ye, Zhiwen, Dudman, Joshua T., Dutta, Barundeb, Hantman, Adam W., Harris, Kenneth D., Lee, Albert K., Moser, Edvard I., O’Keefe, John, Renart, Alfonso, Svoboda, Karel, Häusser, Michael, Haesler, Sebastian, Carandini, Matteo and Harris, Timothy D. (2021) Neuropixels 2.0: a miniaturized high-density probe for stable, long-term brain recordings. Science, 372 (6539), [eabf4588]. (doi:10.1126/science.abf4588).

Record type: Article

Abstract

Measuring the dynamics of neural processing across time scales requires following the spiking of thousands of individual neurons over milliseconds and months. To address this need, we introduce the Neuropixels 2.0 probe together with newly designed analysis algorithms. The probe has more than 5000 sites and is miniaturized to facilitate chronic implants in small mammals and recording during unrestrained behavior. High-quality recordings over long time scales were reliably obtained in mice and rats in six laboratories. Improved site density and arrangement combined with newly created data processing methods enable automatic post hoc correction for brain movements, allowing recording from the same neurons for more than 2 months. These probes and algorithms enable stable recordings from thousands of sites during free behavior, even in small animals such as mice.

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Accepted/In Press date: 1 March 2021
Published date: 16 April 2021

Identifiers

Local EPrints ID: 449117
URI: http://eprints.soton.ac.uk/id/eprint/449117
ISSN: 0036-8075
PURE UUID: eae13d40-e852-4e97-87a5-7bfbf9f0236a
ORCID for Shiwei Wang: ORCID iD orcid.org/0000-0002-5450-2108

Catalogue record

Date deposited: 17 May 2021 16:34
Last modified: 26 Nov 2021 07:19

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Contributors

Author: Nicholas A. Steinmetz
Author: Cagatay Aydin
Author: Anna Lebedeva
Author: Michael Okun
Author: Marius Pachitariu
Author: Marius Bauza
Author: Maxime Beau
Author: Jai Bhagat
Author: Claudia Böhm
Author: Martijn Broux
Author: Susu Chen
Author: Jennifer Colonell
Author: Richard J. Gardner
Author: Bill Karsh
Author: Fabian Kloosterman
Author: Dimitar Kostadinov
Author: Carolina Mora-Lopez
Author: John O’Callaghan
Author: Junchol Park
Author: Jan Putzeys
Author: Britton Sauerbrei
Author: Rik J. J. van Daal
Author: Abraham Z. Vollan
Author: Shiwei Wang ORCID iD
Author: Marleen Welkenhuysen
Author: Zhiwen Ye
Author: Joshua T. Dudman
Author: Barundeb Dutta
Author: Adam W. Hantman
Author: Kenneth D. Harris
Author: Albert K. Lee
Author: Edvard I. Moser
Author: John O’Keefe
Author: Alfonso Renart
Author: Karel Svoboda
Author: Michael Häusser
Author: Sebastian Haesler
Author: Matteo Carandini
Author: Timothy D. Harris

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