The University of Southampton
University of Southampton Institutional Repository

Preconceptional smoking alters spermatozoal miRNAs of murine fathers and affects offspring’s body weight

Preconceptional smoking alters spermatozoal miRNAs of murine fathers and affects offspring’s body weight
Preconceptional smoking alters spermatozoal miRNAs of murine fathers and affects offspring’s body weight
Background: active smoking has been reported among 7% of teenagers worldwide, with ages ranging from 13 to 15 years. An epidemiological study suggested that preconceptional paternal smoking is associated with adolescent obesity in boys. We developed a murine adolescent smoking model before conception to investigate the paternal molecular causes of changes in offspring’s phenotype.

Method: male and female C57BL/6J mice were exposed to increasing doses of mainstream cigarette smoke (CS) from onset of puberty for 6 weeks and mated with room air (RA) controls.

Results: thirteen miRNAs were upregulated and 32 downregulated in the spermatozoa of CS-exposed fathers, while there were no significant differences in the count and morphological integrity of spermatozoa, as well as the proliferation of spermatogonia between CS- and RA-exposed fathers. Offspring from preconceptional CS-exposed mothers had lower body weights (p = 0.007). Moreover, data from offspring from CS-exposed fathers suggested a potential increase in body weight (p = 0.062).

Conclusion: we showed that preconceptional paternal CS exposure regulates spermatozoal miRNAs, and possibly influences the body weight of F1 progeny in early life. The regulated miRNAs may modulate transmittable epigenetic changes to offspring, thus influence the development of respiratory- and metabolic-related diseases such as obesity, a mechanism that warrants further studies for elaborate explanations.
0307-0565
1623-1627
Hammer, Barbara
7006d01d-703c-4df7-a641-f138502c3f5d
Perunthadambil Kadalayil, Latha
e620b801-844a-45d9-acaf-e0a58acd7cf2
Boateng, Eistine
223483a9-2e4f-40bc-820b-27f617de8df9
Buschmann, Dominik
2e6ca9a3-b4e2-47e5-aff0-346bec130d43
Rezwan, Faisal I.
203f8f38-1f5d-485b-ab11-c546b4276338
Wolff, Martin
12bcf4f7-225f-445b-a2ce-0214f36a5d5c
Reuter, Sebastian
dc81bc10-5e98-4f64-b6b8-adba9404225c
Bartel, Sabine
0bec08e5-bca8-4275-af96-760bca7c5ee6
Knudsen, Toril Mørkve
e26d2263-6959-4a49-abb6-f31ce287c1a3
Svanes, Cecilie
4a547f80-e3d3-47b4-ae0a-2741ad93c629
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Krauss-etschmann, Susanne
c7bddda3-701e-468e-8515-fd9722ff8085
Hammer, Barbara
7006d01d-703c-4df7-a641-f138502c3f5d
Perunthadambil Kadalayil, Latha
e620b801-844a-45d9-acaf-e0a58acd7cf2
Boateng, Eistine
223483a9-2e4f-40bc-820b-27f617de8df9
Buschmann, Dominik
2e6ca9a3-b4e2-47e5-aff0-346bec130d43
Rezwan, Faisal I.
203f8f38-1f5d-485b-ab11-c546b4276338
Wolff, Martin
12bcf4f7-225f-445b-a2ce-0214f36a5d5c
Reuter, Sebastian
dc81bc10-5e98-4f64-b6b8-adba9404225c
Bartel, Sabine
0bec08e5-bca8-4275-af96-760bca7c5ee6
Knudsen, Toril Mørkve
e26d2263-6959-4a49-abb6-f31ce287c1a3
Svanes, Cecilie
4a547f80-e3d3-47b4-ae0a-2741ad93c629
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Krauss-etschmann, Susanne
c7bddda3-701e-468e-8515-fd9722ff8085

Hammer, Barbara, Perunthadambil Kadalayil, Latha, Boateng, Eistine, Buschmann, Dominik, Rezwan, Faisal I., Wolff, Martin, Reuter, Sebastian, Bartel, Sabine, Knudsen, Toril Mørkve, Svanes, Cecilie, Holloway, John W. and Krauss-etschmann, Susanne (2021) Preconceptional smoking alters spermatozoal miRNAs of murine fathers and affects offspring’s body weight. International Journal of Obesity, 45 (7), 1623-1627. (doi:10.1038/s41366-021-00798-2).

Record type: Article

Abstract

Background: active smoking has been reported among 7% of teenagers worldwide, with ages ranging from 13 to 15 years. An epidemiological study suggested that preconceptional paternal smoking is associated with adolescent obesity in boys. We developed a murine adolescent smoking model before conception to investigate the paternal molecular causes of changes in offspring’s phenotype.

Method: male and female C57BL/6J mice were exposed to increasing doses of mainstream cigarette smoke (CS) from onset of puberty for 6 weeks and mated with room air (RA) controls.

Results: thirteen miRNAs were upregulated and 32 downregulated in the spermatozoa of CS-exposed fathers, while there were no significant differences in the count and morphological integrity of spermatozoa, as well as the proliferation of spermatogonia between CS- and RA-exposed fathers. Offspring from preconceptional CS-exposed mothers had lower body weights (p = 0.007). Moreover, data from offspring from CS-exposed fathers suggested a potential increase in body weight (p = 0.062).

Conclusion: we showed that preconceptional paternal CS exposure regulates spermatozoal miRNAs, and possibly influences the body weight of F1 progeny in early life. The regulated miRNAs may modulate transmittable epigenetic changes to offspring, thus influence the development of respiratory- and metabolic-related diseases such as obesity, a mechanism that warrants further studies for elaborate explanations.

Text
Hammer et al 2021 Int J Obesity paternal smoking offspring body weight - Version of Record
Available under License Creative Commons Attribution.
Download (1MB)

More information

Accepted/In Press date: 4 March 2021
e-pub ahead of print date: 17 May 2021
Published date: 17 May 2021
Additional Information: Publisher Copyright: © 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

Identifiers

Local EPrints ID: 449415
URI: http://eprints.soton.ac.uk/id/eprint/449415
ISSN: 0307-0565
PURE UUID: 4a1a9208-9756-4dd1-a164-8281316a38fd
ORCID for Faisal I. Rezwan: ORCID iD orcid.org/0000-0001-9921-222X
ORCID for John W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 27 May 2021 16:32
Last modified: 17 Aug 2021 01:45

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×