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Canine mammary cancer tumour behaviour and patient survival time are associated with collagen fibre characteristics

Canine mammary cancer tumour behaviour and patient survival time are associated with collagen fibre characteristics
Canine mammary cancer tumour behaviour and patient survival time are associated with collagen fibre characteristics
Precise diagnosis and prognosis are key in prevention and reduction of morbidity and mortality in all types of cancers. Here we show that changes in the collagen fibres in the main histological subtypes of canine mammary gland carcinomas are directly associated with the tumour behaviour and the animal survival time and could become a useful tool in helping with diagnosis. Imaging by second harmonic generation and multiphoton excited fluorescence microscopy were performed to evaluate the collagen and cellular segment parameters in cancer biopsies. We present a retrospective study of 45 cases of canine mammary cancer analysing 836 biopsies regions including normal mammary gland tissue, benign mixed tumours, carcinoma in mixed tumour, carcinosarcoma, micropapillary carcinoma and solid carcinoma. The image analyses and the comparison between the tumour types allowed to assess the collagen fibre changes during tumour progression. We demonstrate that the collagen parameters correlate with the clinical and pathological data, the results show that in neoplastic tissues, the collagen fibres are more aligned and shorter as compared to the normal tissues. There is a clear association of the mean fibre length with the dogs survival times, the carcinomas presenting shorter collagen fibres indicate a worse survival rate.
2045-2322
Garcia, Ana P.V.
f8f38033-8097-40ae-be69-c9b8c1cbbfc2
Reis, Luana A.
4c7dcb34-71c6-40da-8bbd-00927d01a019
Nunes, Fernanda F.C.
0fba803b-27de-485c-83ef-43f3d5af9e22
Longford, Francis G.J.
27eec433-a773-4cc7-8327-70c5103382e0
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
de Paula, Ana M.
9868f160-10a9-443f-b179-02ca8426003e
Cassali, Geovanni D.
21a1bb65-0f11-4efa-9d19-531724ea1a3c
Garcia, Ana P.V.
f8f38033-8097-40ae-be69-c9b8c1cbbfc2
Reis, Luana A.
4c7dcb34-71c6-40da-8bbd-00927d01a019
Nunes, Fernanda F.C.
0fba803b-27de-485c-83ef-43f3d5af9e22
Longford, Francis G.J.
27eec433-a773-4cc7-8327-70c5103382e0
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
de Paula, Ana M.
9868f160-10a9-443f-b179-02ca8426003e
Cassali, Geovanni D.
21a1bb65-0f11-4efa-9d19-531724ea1a3c

Garcia, Ana P.V., Reis, Luana A., Nunes, Fernanda F.C., Longford, Francis G.J., Frey, Jeremy G., de Paula, Ana M. and Cassali, Geovanni D. (2021) Canine mammary cancer tumour behaviour and patient survival time are associated with collagen fibre characteristics. Scientific Reports, 11 (1), [5668]. (doi:10.1038/s41598-021-85104-w).

Record type: Article

Abstract

Precise diagnosis and prognosis are key in prevention and reduction of morbidity and mortality in all types of cancers. Here we show that changes in the collagen fibres in the main histological subtypes of canine mammary gland carcinomas are directly associated with the tumour behaviour and the animal survival time and could become a useful tool in helping with diagnosis. Imaging by second harmonic generation and multiphoton excited fluorescence microscopy were performed to evaluate the collagen and cellular segment parameters in cancer biopsies. We present a retrospective study of 45 cases of canine mammary cancer analysing 836 biopsies regions including normal mammary gland tissue, benign mixed tumours, carcinoma in mixed tumour, carcinosarcoma, micropapillary carcinoma and solid carcinoma. The image analyses and the comparison between the tumour types allowed to assess the collagen fibre changes during tumour progression. We demonstrate that the collagen parameters correlate with the clinical and pathological data, the results show that in neoplastic tissues, the collagen fibres are more aligned and shorter as compared to the normal tissues. There is a clear association of the mean fibre length with the dogs survival times, the carcinomas presenting shorter collagen fibres indicate a worse survival rate.

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Published date: 11 March 2021
Additional Information: Publisher Copyright: © 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

Identifiers

Local EPrints ID: 449573
URI: http://eprints.soton.ac.uk/id/eprint/449573
ISSN: 2045-2322
PURE UUID: 40ae7e11-b0d6-4e04-96a9-4c74738e1c91
ORCID for Jeremy G. Frey: ORCID iD orcid.org/0000-0003-0842-4302

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Date deposited: 08 Jun 2021 16:31
Last modified: 09 Jun 2021 01:33

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Contributors

Author: Ana P.V. Garcia
Author: Luana A. Reis
Author: Fernanda F.C. Nunes
Author: Francis G.J. Longford
Author: Jeremy G. Frey ORCID iD
Author: Ana M. de Paula
Author: Geovanni D. Cassali

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