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Synthetic nanoclay gels do not cause skin irritation in healthy human volunteers

Synthetic nanoclay gels do not cause skin irritation in healthy human volunteers
Synthetic nanoclay gels do not cause skin irritation in healthy human volunteers
Synthetic clays are promising biomaterials for delivery of therapeutic molecules in regenerative medicine. However, before their use can be translated into clinical applications, their safety must be assessed in human volunteers. The aim of this study was to test the hypothesis that a synthetic nanoclay (LAPONITE) does not cause irritation to the human skin. To achieve this, a nanoclay gel at two different concentrations (1.5 and 3% w/v) was applied on the forearm of healthy volunteers for 24 h. 1% sodium lauryl sulfate (SLS) and 3% (w/v) polyacrylic acid were used as the positive and negative controls, respectively. The compromise in the skin barrier function was measured by trans-epidermal water loss (TEWL), erythema by spectroscopic measurements, and skin inflammatory biomarkers (IL-1α and IL-1RA) by the enzyme-linked immunosorbent assay. We found that the nanoclay caused no prolonged increase in TEWL, erythema, or induction of inflammatory cytokines. This was in contrast to 1% SLS, a known irritant, which induced significant increases in both skin erythema and TEWL. We conclude that the nanoclay is not an irritant and is thus suitable for therapeutic interventions at the skin surface.
hydrogel, irritant, laponite, nanoclay, skin barrier function, skin patch assay
2373-9878
2716-2722
Bostan, Luciana E
9b269056-e210-4ab7-815a-f373528dcd66
Clarkin, Claire
05cd2a88-1127-41aa-a29b-7ac323b4f3c9
Mousa, Mohammed Saleh
315e963d-4ac4-4fad-b6cd-b2c57a5e9e3e
Worsley, Peter
44bc022c-0bea-4df9-bfb7-f3469992bfa1
Bader, Daniel
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Dawson, Jonathan
982bf202-af02-4a6c-9db0-2d4a72c1ea92
Evans, Nicholas
06a05c97-bfed-4abb-9244-34ec9f4b4b95
Bostan, Luciana E
9b269056-e210-4ab7-815a-f373528dcd66
Clarkin, Claire
05cd2a88-1127-41aa-a29b-7ac323b4f3c9
Mousa, Mohammed Saleh
315e963d-4ac4-4fad-b6cd-b2c57a5e9e3e
Worsley, Peter
44bc022c-0bea-4df9-bfb7-f3469992bfa1
Bader, Daniel
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Dawson, Jonathan
982bf202-af02-4a6c-9db0-2d4a72c1ea92
Evans, Nicholas
06a05c97-bfed-4abb-9244-34ec9f4b4b95

Bostan, Luciana E, Clarkin, Claire, Mousa, Mohammed Saleh, Worsley, Peter, Bader, Daniel, Dawson, Jonathan and Evans, Nicholas (2021) Synthetic nanoclay gels do not cause skin irritation in healthy human volunteers. ACS Biomaterials Science & Engineering, 7 (6), 2716-2722. (doi:10.1021/acsbiomaterials.0c01615).

Record type: Article

Abstract

Synthetic clays are promising biomaterials for delivery of therapeutic molecules in regenerative medicine. However, before their use can be translated into clinical applications, their safety must be assessed in human volunteers. The aim of this study was to test the hypothesis that a synthetic nanoclay (LAPONITE) does not cause irritation to the human skin. To achieve this, a nanoclay gel at two different concentrations (1.5 and 3% w/v) was applied on the forearm of healthy volunteers for 24 h. 1% sodium lauryl sulfate (SLS) and 3% (w/v) polyacrylic acid were used as the positive and negative controls, respectively. The compromise in the skin barrier function was measured by trans-epidermal water loss (TEWL), erythema by spectroscopic measurements, and skin inflammatory biomarkers (IL-1α and IL-1RA) by the enzyme-linked immunosorbent assay. We found that the nanoclay caused no prolonged increase in TEWL, erythema, or induction of inflammatory cytokines. This was in contrast to 1% SLS, a known irritant, which induced significant increases in both skin erythema and TEWL. We conclude that the nanoclay is not an irritant and is thus suitable for therapeutic interventions at the skin surface.

Text
Author Accepted Manuscript - Synthetic nanoclay gels do not cause skin irritation in healthy human volunteers - Accepted Manuscript
Restricted to Repository staff only until 7 April 2022.
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More information

Accepted/In Press date: 19 March 2021
e-pub ahead of print date: 7 April 2021
Keywords: hydrogel, irritant, laponite, nanoclay, skin barrier function, skin patch assay

Identifiers

Local EPrints ID: 449686
URI: http://eprints.soton.ac.uk/id/eprint/449686
ISSN: 2373-9878
PURE UUID: 3857f2ba-e372-40c7-9649-41e6d8db6279
ORCID for Luciana E Bostan: ORCID iD orcid.org/0000-0003-3882-2619
ORCID for Daniel Bader: ORCID iD orcid.org/0000-0002-1208-3507

Catalogue record

Date deposited: 10 Jun 2021 16:33
Last modified: 26 Nov 2021 02:57

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Contributors

Author: Luciana E Bostan ORCID iD
Author: Claire Clarkin
Author: Mohammed Saleh Mousa
Author: Peter Worsley
Author: Daniel Bader ORCID iD
Author: Jonathan Dawson
Author: Nicholas Evans

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